A5089714048- Antibiotic Resistance in Bacteria
- Bacterial biofilms and quorum sensing
- Vibrio bacteria research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Enterobacteriaceae and Cronobacter Research
- Antibiotics Pharmacokinetics and Efficacy
- Salmonella and Campylobacter epidemiology
- Infections and bacterial resistance
- Pneumonia and Respiratory Infections
- Urinary Tract Infections Management
- Mycobacterium research and diagnosis
- Antibiotic Use and Resistance
- Escherichia coli research studies
- Plant Pathogenic Bacteria Studies
- Bacterial Identification and Susceptibility Testing
- Bacterial Genetics and Biotechnology
- Pneumocystis jirovecii pneumonia detection and treatment
- Streptococcal Infections and Treatments
- Neutropenia and Cancer Infections
- Bartonella species infections research
- Bacteriophages and microbial interactions
- Tuberculosis Research and Epidemiology
- Nosocomial Infections in ICU
- Rabies epidemiology and control
- Yersinia bacterium, plague, ectoparasites research
Centre d'Immunologie et des Maladies Infectieuses
2014-2023
Sorbonne Université
2013-2023
Assistance Publique – Hôpitaux de Paris
2011-2021
Inserm
1992-2021
Hôpital Saint-Antoine
2003-2021
Hôpitaux Universitaires Paris-Ouest
2012-2019
Centre Hospitalier National Universitaire de Fann
2019
Sorbonne Paris Cité
2018
Université Paris Cité
1994-2017
Hôpital Tenon
2007-2016
To develop a rapid and reliable tool to detect by multiplex PCR assays the most frequently widespread β-lactamase genes encoding OXA-1-like broad-spectrum β-lactamases, extended-spectrum β-lactamases (ESBLs), plasmid-mediated AmpC class A, B D carbapenemases. Following design of specific group primers optimization using control strains, set six PCRs one simplex was created. An evaluation performed collection 31 Enterobacteriaceae strains isolated from clinical specimens showing resistance...
Multidrug-resistant and highly virulent Klebsiella pneumoniae isolates are emerging, but the clonal groups (CGs) corresponding to these high-risk strains have remained imprecisely defined. We aimed identify K. CGs on basis of genome-wide sequence variation provide a simple bioinformatics tool extract virulence resistance gene data from genomic data. sequenced 48 isolates, mostly serotypes K1 K2, compared genomes with 119 publicly available genomes. A total 694 conserved genes were included...
Recently, a CTX-M-15 extended-spectrum β-lactamase (ESBL)-producing Escherichia coli O25b-ST131 clone, belonging to the B2 phylogenetic group and with high virulence potential, has been reported all over world, representing major public health problem. The present study was carried out develop rapid simple detection assay that identifies members of this clone. A total 627 E. isolates which 373 produced an ESBL, collected across four continents, were screened using clone allele-specific PCR...
A clone of CTX-M-15-producing Escherichia coli has recently been reported to be spreading through Europe and Africa. The aim this work was thoroughly characterize clone.Representative isolates were subjected multilocus sequence typing, O virulence gene detection, adhesion assay on human cells, biofilm production mouse lethality assay.The clone: (i) belongs a unique B2 phylogenetic subgroup encompassing the pyelonephritogenic diffusely adhering EC7372 strain; (ii) exhibits specific O25b...
ABSTRACT A single multiplex PCR assay targeting seven virulence factors and the wzi gene specific for K1 K2 capsular serotypes of Klebsiella pneumoniae was developed tested on 65 clinical isolates, which included 45 isolates responsible community-acquired severe human infections. The is useful surveillance emerging highly virulent strains.
Nine clinical isolates of Enterobacteriaceae (six Escherichia coli and three Proteus mirabilis) isolated in Parisian hospitals between 1989 2000 showed a particular extended-spectrum cephalosporin-resistance profile characterized by resistance to cefotaxime aztreonam but not ceftazidime. CTX-M-1, CTX-M-2, CTX-M-9, CTX-M-14 two novel plasmid-mediated CTX-M β-lactamases (CTX-M-20, CTX-M-21) were identified polymerase chain reaction isoelectric focusing (pI>8) associated eight cases with TEM-1...
Objectives: Over a 3 year period (2000–2003) 21 Escherichia coli, 5 Klebsiella pneumoniae, 1 Serratia marcescens and Proteus mirabilis producing CTX-M-type β-lactamase were collected from five different hospitals in Paris, France. This study was conducted to analyse the genetic environment of these 28 blaCTX-M genes.
ABSTRACT Kluyvera ascorbata produces a β-lactamase that results in an atypical susceptibility pattern, including low-level resistance to penicillins, cephalothin, and cefuroxime, but this is reversed by clavulanate. Ten nucleotide sequences of the corresponding gene, bla KLUA , were obtained found have minor variations (96 100%). Otherwise, was be similar (95 100%) some plasmid-encoded CTX-M-type β-lactamases. Finally, mobilization on plasmid mediated probably genetic mobile element like IS Ecp1 .
Colonization of the digestive tract has been supposed to be source many hospital-acquired infections, especially nosocomial pneumonia. To assess relationship between oropharyngeal and gastric colonization subsequent occurrence pneumonia, we prospectively studied 86 ventilated, intensive care unit (ICU) patients. Oropharyngeal or colonizations were detected quantified on admission twice weekly during ICU stay. When pneumonia was suspected clinical grounds (new chest X-ray infiltrate purulent...
Urinary tract infections (UTIs) and acute pyelonephritis (APN) often occur after renal transplantation, but their impact on graft outcome is unclear. One hundred seventy-seven consecutive transplantations were investigated to evaluate the of UTIs APN function. The cumulative incidence was 75.1% that 18.7%. occurred mainly during first year transplantation Escherichia coli, Pseudomonas aeruginosa Enteroccocus sp. most frequent pathogens identified. risk developing higher in female (64%) than...
We analyzed 19 clinical isolates of the family Enterobacteriaceae (16 Escherichia coli and 3 Klebsiella pneumoniae isolates) collected from four different hospitals in Paris, France, 2000 to 2002. These strains had a particular extended-spectrum cephalosporin resistance profile characterized by higher level cefotaxime aztreonam than ceftazidime. The bla(CTX-M) genes encoding these beta-lactamases were involved this resistance, with predominance bla(CTX-M-15). Ten produced both TEM-1-...
ABSTRACT Pantoea agglomerans and other species cause infections in humans are also pathogenic to plants, but the diversity of strains their possible association with hosts disease remain poorly known, identification is difficult. We characterized 36 strains, including 28 diverse origins initially identified as P. , by multilocus gene sequencing based on six protein-coding genes, biochemical tests, antimicrobial susceptibility testing. Phylogenetic analysis comparison Enterobacteriaceae...
Campylobacter bacteremia is uncommon. The influence of underlying conditions and the impact antibiotics on infection outcome are not known.From January 2000 through December 2004, 183 episodes were identified in 23 hospitals Paris, France, area. medical records reviewed. Characteristics due to fetus other species compared. Logistic regression analysis was performed identify risk factors for fatal within 30 days.Most affected patients elderly or immunocompromised. C. most commonly (in 53%...
To assess the implication of genetic background Escherichia coli strains in emergence extendedspectrum β-lactamases (ESBL), 55 TEM-, 52 CTX-M-, and 22 SHV-type ESBL-producing clinical isolates involved various extraintestinal infections or colonization were studied terms phylogenetic group, virulence factor (VF) content (pap, sfa/foc, hly, aer genes), fluoroquinolone resistance.A factorial analysis correspondence showed that SHV type, to a lesser extent TEM preferentially observed B2 group...
ABSTRACT Severe infections caused by hypermucoviscous Klebsiella pneumoniae have been reported in Southeast Asian countries over the past several decades. This report shows their emergence France, with 12 cases observed during a 2-year period two university hospitals. Two clones (sequence type 86 [ST86] and ST380) of serotype K2 five rapidly fatal bacteremia cases, three which were associated pneumonia, whereas seven liver abscess K1 strains ST23.
ObjectivesEscherichia coli producing CTX-M-15 and CTX-M-14 extended-spectrum β-lactamases (ESBLs) are spreading worldwide. The aim of this work was to investigate the replicons involved in emergence spread ESBLs relation ESBL type.
ObjectivesTo characterize the location and genetic environments of qnr, aac(6′)-Ib-cr qepA genes related to quinolone resistance in 19 Escherichia coli, Klebsiella pneumoniae oxytoca strains.
Dissemination of carbapenem resistance among Enterobacteriaceae poses a considerable threat to public health. Carbapenemase gene detection by molecular methods is the gold standard but available in only few laboratories. The aim this study was test phenotypic for metallo-β-lactamase (MBL)- or Klebsiella pneumoniae carbapenemase (KPC)-producing and associated mechanisms β-lactam against panel 30 genotypically characterized carbapenem-resistant : 9 MBL, 7 KPC, 6 OXA-48, 8 extended-spectrum...