A5062861193- Advanced NMR Techniques and Applications
- NMR spectroscopy and applications
- Molecular spectroscopy and chirality
- Trace Elements in Health
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Protein Structure and Dynamics
- Enzyme Structure and Function
- Bacterial Genetics and Biotechnology
- Advanced MRI Techniques and Applications
- Electron Spin Resonance Studies
- Antibiotic Resistance in Bacteria
- Vibrio bacteria research studies
- Crystallography and molecular interactions
- RNA and protein synthesis mechanisms
- Antimicrobial Peptides and Activities
- Enzyme Catalysis and Immobilization
- Lipid Membrane Structure and Behavior
- Bacteriophages and microbial interactions
- Metal complexes synthesis and properties
- Iron Metabolism and Disorders
- Heavy Metal Exposure and Toxicity
- Analytical Chemistry and Chromatography
- Bioactive Compounds and Antitumor Agents
- Microbial Natural Products and Biosynthesis
University of Graz
2016-2025
BioTechMed-Graz
2014-2024
Institute of Bioorganic Chemistry
2019-2023
Medical University of Graz
2023
Nawi Graz
2014-2017
Graz University of Technology
2009
University of Minnesota
1998-2004
Shift work: The new NMR technique, instant homonuclear decoupling, which is achieved by slice-selective homo-decoupling during acquisition, yields pure-shift spectra (see picture) can be recorded like regular 1D spectra. No special data processing necessary and this approach also easily adapted for the direct dimension of two- multidimensional spectra, giving improved resolution. spectroscopy a versatile tool to determine structural, chemical, physical properties molecules. One-dimensional...
Human cathelicidin LL‐37 is a widely studied antimicrobial 37‐mer peptide with various ascribed functions, that serves as template for designing novel peptides. Its derivative, the 24‐mer SAAP‐148, highly effective in vitro eradicating multidrug‐resistant bacteria, persistent cells, and biofilms, without inducing resistance. SAAP‐148 characterized by high cationic charge (+11) 46% hydrophobicity, which, once folds into an alpha helix, forms wide hydrophobic face. This amphipathic nature...
α-Synuclein is an intrinsically unstructured protein that binds to membranes, forms fibrils, and involved in neurodegeneration. We used a reconstituted vitro system show the molecular chaperone Hsp90 influenced α-synuclein vesicle binding amyloid fibril formation, two processes are tightly coupled folding. Binding of monomeric occurred low micromolar range, involving regions critical for amyloidogenesis. As consequence, both were affected. In absence ATP, accumulation non-amyloid oligomers...
Polyhydroxyalkanoates (PHAs) are accumulated in many prokaryotes. Several members of the Halobacteriaceae produce poly-3-hydroxybutyrate (PHB), but it is not known if this a general property family. We evaluated identification methods for PHAs with 20 haloarchaeal species, three them isolates from Permian salt. Staining Sudan Black B, Nile Blue A, or Red was applied to screen presence PHAs. Transmission electron microscopy and (1)H-nuclear magnetic resonance spectroscopy were used...
Many antimicrobial peptides form α-helices when bound to a membrane. In addition, around 80% of residues in membrane-bound proteins are found α-helical regions. The orientation and location such helical the membrane key factors determining their function activity. Here we present new solution state NMR method for obtaining membrane-mimetic environment (micelle-bound) without any chemical perturbation peptide−micelle system. By monitoring proton longitudinal relaxation rates upon addition...
Aims: Protein carbamylation through cyanate is considered as playing a causal role in promoting cardiovascular disease. We recently observed that the phagocyte protein myeloperoxidase (MPO) specifically induces high-density lipoprotein (HDL) carbamylation, rather than chlorination, human atherosclerotic lesions, raising possibility MPO-derived chlorinating species are involved formation. Results: Here, we show rapidly decompose plasma components thiocyanate (SCN) and urea, thereby...
Abstract The nonribosomal enterotoxin tilivalline was the first naturally occurring pyrrolobenzodiazepine to be linked disease in human intestine. Since producing organism Klebsiella oxytoca is part of intestinal microbiota and causes pathogenesis colitis it important understand biosynthesis regulation activity. Here we report show that this peptide assembly pathway initially generates tilimycin, a simple with cytotoxic properties. Tilivalline results from non‐enzymatic spontaneous reaction...
The unique properties of per- and polyfluoroalkyl substances (PFAS) have led to their extensive use in consumer products, including ski wax. Based on the risks associated with PFAS, align PFAS regulations, international federation (FIS) implemented a ban products containing "C
SPECIFIC AIMSWe examined the structural consequences of release metal ions from small, cysteine-rich, binding protein metallothionein (MT) brought about by interaction with nitric oxide (NO). Homonuclear (1H) as well heteronuclear (113Cd) NMR spectroscopy was used to characterize changes associated this potential physiological significance.PRINCIPAL FINDINGS1. Nitric selectively releases metals amino-terminal domain metallothioneinsTo monitor upon metallothionein, a 1 mM mouse...
Abstract Sequential 1 H‐NMR assignments of mouse [Cd 7 ]‐metallothionein‐l (MT1) have been carried out by standard homonuclear NMR methods and the use an accordion‐heteronuclear multiple quantum correlation (HMQC) experiment for establishing metal, 113 Cd 2+ , to cysteine connectivities. The three‐dimensional structure was then calculated using distance constraints from two‐dimensional nuclear Overhauser effect (NOE) spectroscopy spectra Cys‐Cd connectivities as input a geometry‐dynamical...
More information: Relaxation enhancements induced by an inert paramagnetic environment (PREs) are used together with a limited NOE data set in model-free alternative protein structure refinement procedure. Structures of two model systems (8 kDa ubiquitin and the 42 maltodextrin-binding complex β-cyclodextrin) were obtained employing PREs NOEs exchangeable protons.
Human NAD (P)H:quinone oxidoreductase 1 ( NQO 1) is essential for the antioxidant defense system, stabilization of tumor suppressors (e.g. p53, p33, and p73), activation quinone‐based chemotherapeutics. Overexpression in many solid tumors, coupled with its ability to convert chemotherapeutics into potent cytotoxic compounds, have made it a very attractive target anticancer drugs. A naturally occurring single‐nucleotide polymorphism (C609T) leading an amino acid exchange (P187S) has been...
The present study deals with the development of dexamethasone (DM)-loaded implants using ester end-capped Resomer RG 502 poly(lactic acid-co-glycolic acid) (PLGA) (502), acid 502H PLGA (502H), and a 502H:502 mixture (3:1) via hot melt extrusion (HME). prepared intravitreal (20 40% DM loaded in each PLGA) were thoroughly investigated to determine effect different drug loading on long-term release profile DM. characterized for solid-state active pharmaceutical ingredient (APIs) DSC SWAXS,...
The brain specific member of the metallothionein (MT) family proteins, metallothionein-3, inhibits growth and survival neurons, in contrast to ubiquitous mammalian MT isoforms, MT-1 MT-2, that are found most tissues thought function metal ion homeostasis detoxification. Solution NMR was utilized determine structural dynamic differences MT-3 from 2. high-resolution solution structure C-terminal α-domain recombinant mouse revealed a tertiary fold very similar 2, except for loop accommodates an...
ParD is the antidote of plasmid-encoded toxin-antitoxin (TA) system ParD-ParE. These modules rely on differential stabilities a highly expressed but labile and stable toxin from one operon. Consequently, loss coding plasmid results in protective poisoning cell. The protein usually also exhibits an autoregulatory function In this paper, we present solution structure ParD. repressor activity mediated by N-terminal half protein, which adopts ribbon-helix-helix (RHH) fold. C-terminal...
The inherent cytotoxicity of aberrantly folded protein aggregates contributes substantially to the pathogenesis amyloid diseases.It was recently shown that a class evolutionary conserved proteins, called MOAG-4/SERF, profoundly alter toxicity via an autonomous but yet unexplained mode.We show biological function human SERF1a originates from its atypical ability specifically distinguish between and nonamyloid aggregation.This inherently unstructured directly affected aggregation kinetics...