A5027192577- Fungal Infections and Studies
- Plant Pathogens and Fungal Diseases
- Antifungal resistance and susceptibility
- Muscle Physiology and Disorders
- Mycobacterium research and diagnosis
- Adipose Tissue and Metabolism
- Infectious Diseases and Mycology
- melanin and skin pigmentation
- Tuberculosis Research and Epidemiology
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Enterobacteriaceae and Cronobacter Research
- Plant Disease Resistance and Genetics
- Salmonella and Campylobacter epidemiology
- Reproductive System and Pregnancy
- BIM and Construction Integration
- Plant-Microbe Interactions and Immunity
- Yeasts and Rust Fungi Studies
- Escherichia coli research studies
- Viral gastroenteritis research and epidemiology
- Respiratory and Cough-Related Research
- Pediatric Pain Management Techniques
- Biochemical Analysis and Sensing Techniques
- Molecular Biology Techniques and Applications
- Genomics and Phylogenetic Studies
Carnegie Institution for Science
2024
Department of Embryology
2024
University of Wisconsin–Madison
2014-2024
Children's Hospital at Westmead
2012-2023
The University of Sydney
2018-2023
Marshfield Clinic
2011-2022
Marshfield Clinic
2004-2021
WinnMed
2021
Mayo Clinic
2021
Medical College of Wisconsin
2015
Massively parallel DNA sequencing offers many benefits, but major inhibitory cost factors include: (1) start-up (i.e., purchasing initial reagents and equipment); (2) buy-in getting the smallest possible amount of data from a run); (3) sample preparation. Reducing preparation costs is commonly addressed, are rarely addressed. We present dual-indexing systems to address all three these issues. By breaking library construction process into universal, re-usable, combinatorial components, we...
Mycobacterium tuberculosis resides within the phagocytes of its host. It ensures continued survival through arresting normal maturation phagosome, which is retained early endosomal system macrophage. Although individual bacterial components have been shown to modulate phagosome biogenesis, mechanism(s) active in live, intact bacteria remain elusive. We developed a genetic screen that facilitates isolation mutants defective their phagosomes. Macrophages were incubated with iron-dextran was...
Albinism is associated with disrupted foveal development, though intersubject variability becoming appreciated. We sought to quantify this variability, and examine the relationship between cone specialization pit morphology in patients a clinical diagnosis of albinism.We recruited 32 subjects albinism. DNA was obtained from 25 subjects, known albinism genes were analyzed for mutations. Relative inner outer segment (IS OS) lengthening (fovea-to-perifovea ratio) determined manually segmented...
Summary Type‐1 fimbriae are important virulence factors for the establishment of Escherichia coli urinary tract infections. Bacterial adhesion to high‐mannosylated uroplakin Ia glycoprotein receptors bladder epithelium is mediated by FimH adhesin. Previous studies have attributed differences in mannose‐sensitive phenotypes between faecal and uropathogenic E. sequence variation receptor‐binding domain. We find that variants from uropathogenic, enterohaemorrhagic isolates express same...
Alveolar macrophages (AM) are the first professional phagocytes encountered by aerosols containing infections in lungs, and their phagocytic capacity may be affected these or environmental particles. The aim of this study was to evaluate innate endocytic properties human AM obtained from patients with pulmonary tuberculosis characterize vacuoles which Mycobacterium bacilli reside vivo. were bronchoalveolar lavage suspected asymptomatic volunteers (controls). Clinical case definitions based...
Abstract Next-generation DNA sequencing (NGS) offers many benefits, but major factors limiting NGS include reducing costs of: 1) start-up (i.e., doing for the first time); 2) buy-in getting smallest possible amount of data from a run); and 3) sample preparation. Reducing preparation is commonly addressed, are rarely addressed. We present dual-indexing systems to address all three these issues. By breaking library construction process into universal, re-usable, combinatorial components, we...
Blastomyces dermatitidis, the etiologic agent of blastomycosis, has 2 genetic groups and shows varied clinical presentation, ranging from silent infections to fulminant respiratory disease dissemination. The objective this study was determine whether phenotype outcomes vary based on infecting organism's group.We used microsatellites genotype 227 isolates B. dermatitidis Wisconsin patients. For each isolate, corresponding characteristics patient demographic information were abstracted...
Purpose: A hallmark of albinism is foveal hypoplasia. However, literature suggests variable development. This study evaluates the association between ocular phenotype and morphology to demonstrate broad structural functional spectrum. Methods: Best-corrected visual acuity (BCVA), nystagmus, angle kappa, stereoacuity, iris transillumination, macular melanin presence, avascular zone, annular reflex were recorded in 14 patients with albinism. Spectral-domain optical coherence tomography...
Blastomycosis is a potentially fatal infection caused by the fungus Blastomyces dermatitidis. During January 1 through March 5, 2006, twenty-one laboratory confirmed cases of blastomycosis were reported among residents an endemic area in north-central Wisconsin; striking increase compared with previous years. The objective study was to determine if observed urban Wisconsin point-source exposure and identify its source.We epidemiologic features, signs symptoms B. dermatitidis 46 historic...
Blastomyces spp. are endemic in regions of the United States and result blastomycosis, a serious potentially fatal infection. Little is known about presentation, clinic course, epidemiology, genetics blastomycosis children.A retrospective review children with confirmed by culture or cytopathology between 1999 2014 was completed. sp. isolates were genotyped using microsatellite typing, species typed sequencing internal transcribed spacer 2 (its2).Of 114 identified, 79% had isolated pulmonary...
Abstract Cases of blastomycosis, a serious fungal disease globally rare but endemic to North America, can appear both sporadically and in outbreaks. Tracing these outbreaks their environment has traditionally used culturing polymerase chain reaction. Here, we present our method for metagenomic detection Blastomyces 2015 outbreak soil sample from central Wisconsin. By sequencing this multiple depths, simulated the minimum required depth detect outbreak. Our methods recommendations be identify...
Alexandra M. Linz, PhD*⇑, Holly Frost, MD†, Terrie Kitchner*, Jennifer L. Anderson, BS‡ and Meece, PhD* *Marshfield Clinic Research Institute, Marshfield, WI USA †Department of Pediatrics, Denver Health Hospital Authority, Denver, CO USA; Center for Services Research, Department University Colorado School Medicine, Aurora, ORCID: 0000-0002-3016-3287 ‡Department Pathology Laboratory Wisconsin-Madison, Corresponding Author: PhD, Marshfield Foundation, 1000 N Oak Ave, 54449, Email:...
This study documents the first reported transmission of Salmonella enterica serotype Typhimurium definitive type 104 (DT104) to premature fraternal twins via their mother’s breast milk. When twin neonates developed severe enteritis in neonatal intensive care unit (NICU), stool samples and milk were cultured for presence Salmonella. Antibacterial susceptibility patterns determined. Semiquantitative organism abundance data retrospectively gathered on 54 stored collected 34 different days using...
Blastomycosis is a serious and potentially fatal infection caused by the thermally dimorphic fungus Blastomyces dermatitidis. Polymerase chain reaction (PCR) assays targeting BAD-1 virulence gene promoter have been developed to aid in detection of pathogen clinical environmental samples. However, little known regarding genetic diversity B. dermatitidis how this might affect performance characteristics these assays. We explored relatedness 106 isolates using previously described rDNA PCR...
Blastomyces dermatitidis, a thermally dimorphic fungus, is the etiologic agent of North American blastomycosis. Clinical presentation varied, ranging from silent infections to fulminant respiratory disease and dissemination skin other sites. Exploration population genetic structure B. dermatitidis would improve our knowledge regarding variation in virulence phenotypes, geographic distribution, difference host specificity. The objective this study was develop test panel microsatellite markers...
Summary Background: Bilateral myringotomy (BMT) is a commonly performed otolaryngologic procedure in children. Objectives: To examine the effects of intranasal dexmedetomidine, an α 2 ‐adrenoceptor agonist, on time‐averaged pain scores, control, need for rescue analgesia, and agitation scores children undergoing BMT. Methods: We designed trial to enroll 160 randomized one four groups: two study groups, dexmedetomidine (1 or μg·kg −1 ), control groups representing our institutional standards...
Dopamine is an intermediate product in the biosynthesis of melanin pigment, which absent or reduced albinism. Animal research has shown that supplying a precursor to dopamine, levodopa, may improve visual acuity albinism by enhancing neural networks. This study examines safety and effectiveness levodopa on best-corrected human subjects with albinism.Prospective, randomized, placebo-controlled, double-masked clinical trial conducted at University Minnesota.Forty-five albinism.Subjects were...
Mycobacterium avium subspecies paratuberculosis (MAP) has been suspected of involvement in Crohn's disease (CD). We investigated this potential association by testing whole blood from CD patients and healthy controls for the presence MAP culture molecular methods. In addition, each sample was analyzed polymorphisms NOD2/CARD15 gene previously associated with CD. Four 4-mL K2-EDTA tubes were drawn subject (n = 260, 130 controls). Two cultured following methods: Mycobacterial Growth Indicator...
The presence of Mycobacterium avium subsp. paratuberculosis (MAP) in non-ruminant wildlife has raised questions regarding the role these species Johne's disease transmission. In this study we tested 472 tissues from 212 animals six different scavenging mammals. All were taken within a 210-square-mile area Dane and Iowa counties south central Wisconsin September to May 2003-04 for MAP. We detected MAP-specific DNA 81 (38%) mammals, 98 (21%) tissues; viable MAP was cultured one coyote's ileum...