A5001759370- Herpesvirus Infections and Treatments
- Toxoplasma gondii Research Studies
- CRISPR and Genetic Engineering
- Viral Infections and Vectors
- Virus-based gene therapy research
- Parasitic Infections and Diagnostics
- Hepatitis B Virus Studies
- Advanced biosensing and bioanalysis techniques
- Animal Virus Infections Studies
- Computational Drug Discovery Methods
- Bioinformatics and Genomic Networks
- Viral Infections and Outbreaks Research
- Rabies epidemiology and control
- Plant Virus Research Studies
- Mosquito-borne diseases and control
- DNA Repair Mechanisms
- Microbial Metabolic Engineering and Bioproduction
- Virology and Viral Diseases
- Genetic Mapping and Diversity in Plants and Animals
- Microtubule and mitosis dynamics
- Biofuel production and bioconversion
- Immunotherapy and Immune Responses
- Viral Infections and Immunology Research
- Microbial infections and disease research
- Cytomegalovirus and herpesvirus research
Jiangsu Provincial Center for Disease Control and Prevention
2024
Chengdu Center for Disease Control and Prevention
2024
Hainan Medical University
2023
The Affiliated Yongchuan Hospital of Chongqing Medical University
2023
Chongqing Medical University
2023
University of Arizona
2021
Changsha University of Science and Technology
2021
Fujian Medical University
2020
Union Hospital
2020
Shanghai Municipal Center For Disease Control Prevention
2009-2019
Purpose: To explore the mechanism underlying IL-8-induced neutrophil extracellular trap (NET) formation in patients with ocular-active Behçet's disease (BD) and effect of inhibiting NET on severity inflammation experimental autoimmune uveitis (EAU) mice. Methods: The serum DNA elastase (NE) IL-8 levels BD, expression myeloperoxidase, NE, histone H3Cit IL-8–induced neutrophils isolated from healthy controls, effects NETs HMC3 cells were detected. Female C57BL/6J mice immunized IRBP651–670 to...
This study investigates the impact of physical education (PE) instructors’ critical thinking skills on students’ learning in a business college Wuhan, China. The research examined across three dimensions: evaluating information, creative thinking, and problem-solving. Utilizing descriptive design, data were collected through validated questionnaire distributed to student respondents. findings revealed that PE instructors demonstrate high level thinking. Key indicators, such as identifying...
Toxoplasma gondii is an obligate intracellular parasite which can infect almost all mammalian animals, leading to toxoplasmosis. T. rhoptry protein 38 (TgROP38) active kinase involved in the inhibitory effect on host cell transcription by down-regulating MAPK signaling track. TgROP38 gene was amplified and inserted into eukaryotic vector pVAX I formed DNA vaccine pVAX-ROP38. Mice experimental group were intramuscularly immunized with pVAX-ROP38 those injected I, PBS or nothing treated as...
Background SFTS virus (SFTSV) is a newly discovered pathogen to cause severe fever with thrombocytopenia syndrome (SFTS) in human. Successful control of SFTSV epidemic requires better understanding the antigen target humoral immune responses new bunyavirus infection. Methodology/Principal Findings We have generated combinatorial Fab antibody phage library from two patients recovered To date, 94 unique human antibodies been and characterized over 1200 clones obtained by screening purified...
Endonuclease V is a deoxyinosine 3'-endonuclease which initiates removal of inosine from damaged DNA. A thermostable endonuclease the hyperthermophilic bacterium Thermotoga maritima has been cloned and expressed in Escherichia coli. The DNA recognition reaction mechanisms were probed with both double-stranded single-stranded oligonucleotide substrates contained inosine, abasic site (AP site), uracil, or mismatches. Gel mobility shift kinetic analyses indicate that enzyme remains bound to...
Toxoplasma gondii can infect almost all warm-blood animals including human beings. The plant-like calcium-dependent protein kinases (CDPKs) harbored by T. are involved in gliding motility, cell invasion, egress and some other developmental processes, so have been implicated as important virulence factors.In the present study, we constructed a DNA vaccine expressing CDPK3 (TgCDPK3) evaluated its protective efficacy against infection Kunming mice. gene sequence encoding TgCDPK3 was inserted...
Previous studies have compared the physicochemical properties of allosteric compounds to non-allosteric compounds. Those found that tend be smaller, more rigid, hydrophobic, and drug-like than However, previous not properly corrected for fact some protein targets much data other systems. This generates concern regarding possible skew can introduced by inherent bias in available data. Hence, this study aims determine how robust findings are addition newer utilizes Allosteric Database (ASD...
Motivation: What if you could explain complex chemistry in a simple tree and share that data online with your collaborators? Computational biology often incorporates diverse chemical to probe biological question, but the existing tools for are ill-suited very large datasets inherent bioinformatics. Furthermore, visualization methods require an expert chemist interpret patterns. Biologists need interactive tool visualizing information intuitive, accessible way facilitates its integration into...
Safe and efficient gene transfer systems are the basis of therapy applications. Non-integrating lentiviral (NIL) vectors among most promising candidates for tools, because they exhibit high efficiency in both dividing non-dividing cells do not present a risk insertional mutagenesis. However, non-integrating cannot introduce stable exogenous expression to cells, thereby limiting their application. Here, we report design vector that contains minimal scaffold/matrix attachment region (S/MAR)...
Magnetic MXene composite Fe3O4@Ti3C2 was successfully prepared and employed as 17α-ethinylestradiol (EE2) adsorbent from water solution. The response surface methodology to investigate the interactive effects of adsorption parameters (adsorption time, pH solution, initial concentration, dose) optimize these for obtaining maximum efficiency EE2. significance independent variables their interactions were tested by analysis variance (ANOVA) t-test statistics. Optimization process EE2 performed...
DNA strand joining entails three consecutive steps: enzyme adenylation to form AMP−ligase, substrate AMP−DNA, and nick closure. In this study, we investigate the effects on ligation steps by deletion site-directed mutagenesis of BRCA1 C-terminal (BRCT) domain using NAD+-dependent ligase from Thermus species AK16D. Deletion BRCT resulted in substantial loss activity, but mutant was still able an AMP−ligase intermediate, suggesting that defects caused entire occur primarily at after...
Toxoplasma gondii is an intracellular parasite that causes a long-term latent infection of neurons. Using custom MATLAB-based mapping program in combination with mouse model allows us to permanently mark neurons injected proteins, we found -injected (TINs) are heterogeneously distributed the brain, primarily localizing cortex followed by striatum. In addition, determined cortical TINs commonly (>50%) excitatory (FoxP2 + ) and striatal often (>65%) medium spiny (MSNs) ). By performing...
has a broad host range and caused lethal bubonic pneumonic plague in humans. With the emergence of multiple resistant strains potential for biothreat use, there is an urgent need new therapeutic strategies that can protect populations from natural or deliberate infection. Targeting F1 been proven to be main strategy developing vaccines antibodies, but data on anti-F1 especially humans, are scarce. To date, three human monoclonal antibodies (m252, αF1Ig2, αF1Ig8) naive have reported. Here, we...
Endonuclease V nicks damaged DNA at the second phosphodiester bond 3' to inosine, uracil, mismatched bases, or abasic (AP) sites. Alanine scanning mutagenesis was performed in nine conserved positions of Thermotoga maritima endonuclease identify amino acid residues involved recognition endonucleolytic cleavage these diverse substrates. substitution D43, E89, and D110 either abolishes substantially reduces inosine activity. These three mutants gain binding affinity for double-stranded...
Polo-like kinase 1 (PLK1), one of the key regulators mitosis, is a target for cancer therapy due to its abnormally high activity in several tumors. Plk1 highly conserved and shares nearly identical 3-D structure between zebrafish humans. The initial 10 mitoses embryonic cleavages occur every∼30 minutes, therefore provide rapid assay evaluate mitosis inhibitors including those targeting Plk1. To increase efficiency specificity, we first performed computational virtual screen of∼60000...
During the preclinical drug discovery process it remains a challenge to enable early elimination of candidate molecules that may have non-specific, off-target activities. Here, we use whole zebrafish embryo assays coupled with genetic analysis address this issue. PLK1 (Polo-like kinase 1) is one key regulators control mitotic entry, spindle assembly, chromosome segregation, and cytokinesis in cell cycle. Since plk1 expression abnormally up-regulated several tumors, regarded as good target...
Toxoplasma gondii causes one of the most common protozoal diseases humans and animals worldwide. With aim designing an effective vaccine against T. infection, we examined immunogenicity a DNA expressing heat shock protein 40 (HSP40) challenge with (type I RH type II Pru) strains in Kunming mice. The plasmid pVAX1-HSP40 was constructed used to immunize mice by intramuscular injection for three sequential immunizations two-week intervals. This immunization regimen significantly reduced...