A5100352744- Cervical Cancer and HPV Research
- Genital Health and Disease
- Reproductive tract infections research
- Hepatitis B Virus Studies
- Global Cancer Incidence and Screening
- Molecular Biology Techniques and Applications
- Endometrial and Cervical Cancer Treatments
- CRISPR and Genetic Engineering
- Cardiac Arrhythmias and Treatments
- Gut microbiota and health
- Metabolomics and Mass Spectrometry Studies
- Cardiac electrophysiology and arrhythmias
- Cardiac pacing and defibrillation studies
- bioluminescence and chemiluminescence research
- Spinal Dysraphism and Malformations
- Genetics, Aging, and Longevity in Model Organisms
- Cancer-related molecular mechanisms research
- Pluripotent Stem Cells Research
- Carcinogens and Genotoxicity Assessment
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Mass Spectrometry Techniques and Applications
- Head and Neck Surgical Oncology
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
Chinese Academy of Medical Sciences & Peking Union Medical College
2014-2023
Peking Union Medical College Hospital
2020
Fogarty International Center
2017
Yunnan Maternal and Child Health
2017
University of North Carolina at Chapel Hill
2017
University of Chicago
2017
Cancer Institute (WIA)
2013
Objective To profile gut microbiome-associated metabolites in serum and investigate whether these could distinguish individuals with colorectal cancer (CRC) or adenoma from normal healthy individuals. Design Integrated analysis of untargeted metabolomics by liquid chromatography-mass spectrometry metagenome sequencing paired faecal samples was applied to identify significantly altered abundance patients CRC adenoma. The ability discriminate between tested targeted metabolomic analysis. A...
Abstract High‐risk (HR) human papillomavirus (HPV) prevalence has been shown to correlate well with cervical cancer incidence rates. Our study aimed estimate the of HR‐HPV and intraepithelial neoplasia (CIN) in China indirectly informs on burden country. A total 30,207 women from 17 population‐based studies throughout were included. All received HPV DNA testing (HC2, Qiagen, Gaithersburg, MD), visual inspection acetic acid liquid‐based cytology. Women positive for any test...
We Sought to identify circulating miRNAs suitable for HF diagnosis.In this study, a genome-wide plasma miRNA microarray was performed in 13 patients and 3 controls. The expression levels of selected differentially expressed, upregulated (miR-3135b, miR-3908 miR-5571-5p) were validated with quantitative real-time PCR (qRT-PCR) assays an independent cohort 33 20 controls.Of all the analyzed, miR-3135b (P<0.001), miR-5571-5p (P<0.001) found have significantly different compared Receiver...
Abstract Mouse somatic cells can be reprogrammed into induced pluripotent stem by defined factors known to regulate pluripotency, including Oct4, Sox2, Klf4, and c-Myc. Together with Sox2 plays a major role as master endogenous genes trigger in reprogramming. It has been reported that Sirtuin 1 (Sirt1), member of the family NAD+-dependent protein deacetylases, is involved embryonic cell antioxidation, differentiation, individual development. However, deacetylation enzyme, whether Sirt1...
In China, high-risk human papillomavirus (HR-HPV) prevalence is unexpectedly high in older women, but the possible reasons have not been well studied yet. This study investigated age trends of HR-HPV infection a prospective study. A total 7397 women aged 25-65 years without cervical precancer or cancer were evaluated during 2010-2011 with stratified sample 2791 re-evaluated after one year. Test results for careHPV and careHPV16/18/45 used to describe prevalence, incidence clearance. Risk...
This study examined the efficacy of OncoE6™ Cervical Test, careHPV™ and visual inspection with acetic acid (VIA) in identifying women at risk for cervical cancer their capability to detect incident precancer 1-year follow-up. In a population 7,543 living rural China, provided self-collected two clinician-collected specimens underwent VIA. All screen positive any tests, ∼ 10% random sample test-negative that colposcopy baseline, an additional who did not undergo baseline (n = 3,290) were...
Abstract We previously reported the results of a phase II/III, double‐blind, randomized controlled study in Chinese women (NCT00779766) showing 94.2% (95% confidence interval: 62.7–99.9) HPV‐16/18 AS04‐adjuvanted vaccine efficacy (VE) against cervical intraepithelial neoplasia grade 1 or higher (CIN1+) and/or 6‐month (M) persistent infection (PI) with mean follow‐up <2 years, and immunogenicity until 7 months post‐dose 1. Here, we report safety from an event‐triggered analysis ~3 years...
Abstract Aims The present study was to evaluate the feasibility and clinical outcomes of left bundle branch area pacing (LBBAP) in cardiac resynchronization therapy (CRT)-indicated patients. Methods results LBBAP performed via transventricular septal approach 25 patients as a rescue strategy 5 with failed ventricular (LV) lead placement primary remaining 20 Pacing parameters, procedural characteristics, electrocardiographic, echocardiographic data were assessed at implantation follow-up. Of...
Abstract Background The causal role of high-risk Human papillomavirus (HR-HPV) in the pathogenesis anogenital cancers is well established. In contrast, information on HR-HPV distribution continuous anatomic sites within female genital tract limited, and impact sample type clinical performance HPV-based cervical cancer screening warrants investigation. Methods A total 2,646 Chinese women were enrolled study from May 2006 to April 2007. We analyzed infection features by status pathological...
Precise prevention is more desired for cervical cancer due to the huge population, high prevalence of human papillomavirus (HPV) infection in China and vision screen-and-treat strategies low- middle-income countries (LMICs). Considerations combining type-specific attribution proportion high-grade intraepithelial neoplasia are informative precise effective region-specific control programs. The aim current study was determine genotype distribution HPV precancerous lesions among women from...
Abstract The causal relationship between persistent high‐risk human papillomavirus infection and cervical cancer is widely accepted. HR‐HPV DNA testing, alone or in combination with Pap smear may have a role primary screening. screening results (VIA, VILI, Pap, DNA) of 9,057 women rural China were analyzed to determine the performance for detection CIN3+. All strategies had comparable AUCs (0.9). Cotesting overall highest sensitivity CIN3+ (99.4%), followed by testing (96.3%), (80.2%),...
This study aims to evaluate human papillomavirus (HPV) viral loads as a biomarker for triage into colposcopy and cervical intraepithelial neoplasia grade 2 (CIN2) therapy reduce the referral rate CIN2 overtreatment in low-resource settings.In 1999, 1997 women aged 35 45 years Shanxi, China, received 6 screenings with pathological confirmation. In 2005, 1461 histologically normal women, 99 CIN 1 (CIN1), 30 or worse (CIN2+) were rescreened follow-up study. Human was detected by Hybrid Capture...
Abstract Improvement in managing HPV‐positive women is urgently needed. Based on a population‐based study which included 2112 aged 49 to 69 from Shanxi, China, we aimed evaluate the clinical performance of multiple triage strategies based liquid‐based cytology (LBC), p16 INK4a , viral load and partial genotyping, as single or combined strategy for detecting cervical intraepithelial neoplasia grade 2/3 higher (CIN2+/CIN3+) who tested positive by Hybrid Capture 2 (HC2). Among 452 HC2‐positive...
To examine the influence of human papillomavirus (HPV) genotypes on sensitivity visual inspection with acetic acid (VIA) for screening, and colposcopy diagnosis cervical intraepithelial neoplasia grade 2 (CIN2) or more severe (CIN2+).Women aged 25 to 65 years from China (n = 7,541) were screened 6 tests (careHPV Hybrid Capture self- clinician-collected specimens; HPV-16, HPV-18, HPV-45 E6 detection; VIA). Biopsies women a CIN2+ underwent testing HPV using SPF10/LiPA. Human genotyping results...
ABSTRACT careHPV, a lower-cost DNA test for human papillomavirus (HPV), is being considered cervical cancer screening in low- and middle-income countries. However, not single large-scaled study exists to investigate the optimal positive cutoff point of careHPV test. We pooled data 9,785 women participating two individual studies conducted from 2007 2011 rural China. Woman underwent multiple tests, including on clinician-collected specimens (careHPV-C) self-collected (careHPV-S), Hybrid...
Safe and efficient gene transfer systems are the basis of therapy applications. Non-integrating lentiviral (NIL) vectors among most promising candidates for tools, because they exhibit high efficiency in both dividing non-dividing cells do not present a risk insertional mutagenesis. However, non-integrating cannot introduce stable exogenous expression to cells, thereby limiting their application. Here, we report design vector that contains minimal scaffold/matrix attachment region (S/MAR)...
Abstract Background It is widely acknowledged that HPV prophylactic vaccine could prevent new infections and their associated lesions among women who are predominantly HPV-naive at vaccination. Yet there still remains uncertainty about whether vaccination benefit to individuals have undergone surgery for cervical disease. Methods This post-hoc analysis intends focus on intent-to-treat participants underwent excision treatment baseline the follow-up period in a phase II/III, double-blind,...
Abstract Objective: CareHPV is a human papillomavirus (HPV) DNA test for low-resource settings (LRS). This study assesses optimum triage strategies careHPV-positive women in LRS. Methods: A total of 2,530 Chinese were concurrently screened cervical cancer with visual inspection acetic acid (VIA), liquid-based cytology and HPV testing by physician- self-collected careHPV, physician-collected Hybrid Capture 2 (HC2). Screen-positive referred to colposcopy biopsy endocervical curettage as...
Liquid-state specimen carriers are inadequate for sample transportation in large-scale screening projects low-resource settings, which necessitates the exploration of novel non-hazardous solid-state alternatives. Studies investigating feasibility and accuracy a human papillomavirus (HPV) sampling medium combination with different down-stream HPV DNA assays cervical cancer needed.We collected two specimens from 396 women, aged 25-65 years, who were enrolled trial. One was stored using DCM...
We aimed to verify the expression status and diagnostic significance of isocitrate dehydrogenase 1 (IDH1) in non-small-cell lung cancer (NSCLC), especially during early stages. Serum IDH1 levels were measured by ELISA. A total 1223 participants (660 patients with NSCLC, 276 healthy controls [HCs], 95 benign pulmonary conditions [BPCs], 135 other cancers [OCs], 57 samples interfering factors) divided into a training cohort validation according 3 testing centers. The concentrations NSCLC group...