Polycystic ovarian syndrome (PCOS) is a complex endocrinopathy in women of reproductive age and the main cause female infertility, but there no universal drug for PCOS therapy. As predominant dietary isoflavone present soybeans, genistein (GEN) possesses estrogenic antioxidative properties, limited information available regarding its therapeutic potential underlying molecular mechanism PCOS. In this study, we found that GEN might restore estrous cycle mice ameliorate elevation circulating T,...

Polycystic ovarian syndrome (PCOS) is one of the most prevalent endocrinopathies and leading cause anovulatory infertility, but its pathogenesis remains elusive.Although HB-EGF involved in cancer progression, there still no clarity about relevance with PCOS.The present study exhibited that abundant was noted follicular fluid from PCOS women, where it might induce granulosa cells (GCs) production more estrogen via elevation CYP19A1 expression after binding to EGFR.Furthermore, transduced...

Abstract Early growth response gene 1 (Egr1), a zinc finger transcriptional factor, plays an important role in regulating cell proliferation, differentiation and angiogenesis. Current data have shown that Egr1 is involved follicular development, ovulation, luteinization placental However, the expression, regulation function of mouse uterus during embryo implantation decidualization are poorly understood. Here we showed was strongly expressed subluminal stroma surrounding implanting...

Abstract TAZ, as a crucial effector of Hippo pathway, is required for spermatogenesis and fertilization, but little known regarding its physiological function in uterine decidualization. In this study, we showed that TAZ was localized the decidua, where it promoted stromal cell proliferation followed by accelerated G1/S phase transition via Ccnd3 Cdk4 induced expression or activity differentiation markers Prl8a2, Prl3c1 ALP, indicating importance Knockdown impeded HB-EGF induction...

This goal of this study was to examine immunohistochemical distribution leukemia inhibitory factor (LIF), LIF receptor (LIFR), and glycoprotein (gp) 130 in rhesus monkey uterus during the menstrual cycle early pregnancy. Pregnancy rate significantly reduced control group from 66.7% (12 18) 22.2% (4 with an injection goat anti-human recombinant immunoglobulin G into uterine lumen on Day 8 mainly localized glandular luminal epithelium. immunostaining luteal phase stronger than it proliferative...

HB-EGF is essential for uterine decidualization, but its antioxidant function remains largely unclear. Here, we found that promoted the proliferation of stromal cells followed by accelerated transition cell cycle from G1 to S phase and enhanced expression or activity Prl8a2, Prl3c1, ALP which were well-established markers differentiation during decidualization. Under oxidative stress, was impaired, this impairment abrogated rHB-EGF accompanied with reduced levels ROS MDA regarded as...

Abstract Objectives Chondrocyte proliferation and differentiation are crucial for endochondral ossification, but their regulatory mechanism remains unclear. The present study aimed to determine the physiological function of TGFβ1 signalling in antler chondrocytes explore its relationship with Notch, Shh Foxa. Materials methods Immunofluorescence, Western blot, MTS assay, flow cytometry, RNA interference real‐time PCR were used analyse mechanisms chondrocyte differentiation. Results TGFβ1,...

Yap is required for ovarian follicle and early embryo development, but little information available regarding its physiological significance in decidualization.Here we determine the effects of YAP on decidualization, mitochondrial function, cell apoptosis DNA damage, explore interplay with Bmp2, Rrm2, GSH ROS.The results exhibited that was abundant decidual cells inactivation impaired proliferation differentiation stromal along deferral G1/S phase transition, indicating importance...

Although IGF1 is important for the proliferation and differentiation of chondrocytes, its underlying molecular mechanism still unknown. Here we addressed physiologic function in antler cartilage explored interplay IGF1, IRS1/2 RUNX1 chondrocyte differentiation. The results showed that was highly expressed chondrocytes. Exogenous rIGF1 could increase chondrocytes cell proportion S phase, whereas IGF1R inhibitor PQ401 abrogated induction by rIGF1. Simultaneously, stimulate expression IHH which...

Tryptophan 2,3-dioxygenase (Tdo2) is a rate-limiting enzyme which directs the conversion of tryptophan to kynurenine. The aim this study was examine expression and regulation Tdo2 in mouse uterus during decidualization. mRNA mainly expressed decidua on days 6-8 pregnancy. By real-time PCR, high level observed uteri from 6 8 pregnancy, although 1-8. Simultaneously, also detected under vivo vitro artificial Estrogen, progesterone, 8-bromoadenosine-cAMP could induce ovariectomized uterine...

Genistein is an isoflavone that has estrogen (E2 )-like activity and beneficial for follicular development, but little known regarding its function in oxidative stress (OS)-mediated granulosa cell (GC) injury. Here, we found after exposure to H2 O2 , weakened the elevated levels of intracellular reactive oxygen species (ROS) malondialdehyde (MDA), which were regarded as biomarkers OS, rescued glutathione (GSH) content GSH/GSSG ratio accompanying with a simultaneous increase cyclic adenosine...

Although ATRA is involved in regulating the proliferation and differentiation of chondrocytes, its underlying mechanism remains unknown. Here we showed that could stimulate antler chondrocytes expression COL X MMP13 which were two well‐known markers for hypertrophic chondrocytes. Silencing CRABP2 prevented induction on chondrocyte terminal differentiation, while overexpression exhibited opposite effects. CYP26A1 CYP26B1 weakened sensitivity to ATRA. Further analysis evidenced might induce...

Although Gja1 has been proved to play an important role in uterine decidualization, its regulatory mechanism remains largely unknown. Here, we showed that was highly expressed the decidual cells and promoted proliferation of stromal expression Prl8a2 Prl3c1, which were two well-known differentiation markers for decidualization. Further analysis revealed might act downstream Acvr1 cAMP regulate cells. Administration analog 8-Br-cAMP siRNA-transfected resulted obvious increase expression,...

Ptn is a pleiotropic growth factor involving in the regulation of cellular proliferation and differentiation, but its biological function uterine decidualization remains unknown. Here, we showed that was highly expressed decidual cells, could induce stromal cells expression Prl8a2 Prl3c1 which were two well-established differentiation markers for decidualization, suggesting an important role decidualization. In progesterone stimulated accompanied with accumulation intracellular cAMP level....

Although Hmgn1 is involved in the regulation of gene expression and cellular differentiation, its physiological roles on differentiation uterine stromal cells during decidualization still remain unknown. Here we showed that mRNA was highly expressed decidua days 6-8 pregnancy. Simultaneously, increased also observed artificial vitro induced models. proliferation Ccna1, Ccnb1, Ccnb2 Cdk1 absence estrogen progesterone. Overexpression could enhance Prl8a2 Prl3c1 which were 2 well-known markers...

For the purpose of investigation working mechanisms in endocrine disruptors, we evaluated dose-related effects fetal and/or neonatal exposure to an estrogenic compound on male reproductive organs adult mice, particularly with respect gene expression steroidogenic acute regulatory protein (StAR). The pregnant ICR mice were given subcutaneous injections 10 μg/day/animal diethylstilbestrol (DES) subject utero (IUE). Subsequently, newborn subjected (NE) by treatment vehicle or 0.1-10 DES....

What is the central question of this study? are potential therapeutic roles ginsenoside Rb1 and hydroxysafflor yellow A (HSYA) in polycystic ovary syndrome (PCOS). main finding its importance? HSYA restored oestrous cycles PCOS mice, reduced follicular cysts ovaries rescued abnormal hormone secretion; did not ameliorate symptoms mice. alleviated oxidative stress along with an enhancement antioxidant enzyme activity. This highlights a role therapy.Polycystic (PCOS) most common endocrine...

Although Hmgn5 is involved in the regulation of cellular proliferation and differentiation, its physiological function during decidualization still unknown. Here we showed that was highly expressed decidual cells. Silencing expression by specific siRNA reduced uterine stromal cells Ccnd3 Cdk4 absence or presence estrogen progesterone, whereas overexpression exhibited opposite effects. Simultaneously, might induce Prl8a2 Prl3c1 which were 2 well-known differentiation markers for...

Abstract Polycystic ovary syndrome (PCOS) is the primary cause of female infertility with a lack universal therapeutic regimen. Although osthole exhibits numerous pharmacological activities in treating various diseases, its effect on PCOS undiscovered. The present study found that application improved symptoms mice through preventing ovarian granulosa cells (GCs) production more estrogen and alleviating liberation pro‐inflammatory cytokine interleukin (IL)‐1β, IL‐6, tumor necrosis factor...

The aim of this study was to examine the expression and regulation crystallin, alpha B (Cryab) gene in mouse uterus during peri-implantation period by situ hybridization real-time PCR. There no detectable Cryab mRNA signal on days 1-4 pregnancy. On day 5 pregnancy when embryo implanted, a high level found subluminal stroma surrounding implanting blastocyst. 6-8, strongly expressed primary decidua. By PCR, detected 7 8 pregnancy, although seen from 1 8. Under vivo vitro artificial...