A5100440567- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Animal Disease Management and Epidemiology
- Cancer-related molecular mechanisms research
- Vector-Borne Animal Diseases
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Circular RNAs in diseases
- Cancer therapeutics and mechanisms
- Plant Virus Research Studies
- MicroRNA in disease regulation
- Reproductive System and Pregnancy
- Reproductive Physiology in Livestock
- Genetic and phenotypic traits in livestock
- Effects of Environmental Stressors on Livestock
- DNA Repair Mechanisms
- Genetics, Bioinformatics, and Biomedical Research
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Angiogenesis and VEGF in Cancer
- Growth Hormone and Insulin-like Growth Factors
- Solar Radiation and Photovoltaics
- RNA regulation and disease
- Autophagy in Disease and Therapy
- Berberine and alkaloids research
Chinese Academy of Agricultural Sciences
2015-2024
Hunan Agricultural University
2020-2024
Harbin Veterinary Research Institute
2015-2024
Qingdao Agricultural University
2023-2024
Zhongnan Hospital of Wuhan University
2022
Wuhan University
2022
Henan Provincial People's Hospital
2020-2021
Zhengzhou University
2021
Zhengzhou Central Hospital
2020
Wenzhou University
2020
Abstract Poly(ADP-ribose) polymerases (PARP) attach poly(ADP-ribose) (PAR) chains to various proteins including themselves and chromatin. Topoisomerase I (Top1) regulates DNA supercoiling is the target of camptothecin indenoisoquinoline anticancer drugs, as it forms Top1 cleavage complexes (Top1cc) that are trapped by drugs. Endogenous carcinogenic lesions can also trap Top1cc. Tyrosyl-DNA phosphodiesterase 1 (TDP1), a key repair enzyme for Top1cc, hydrolyzes phosphodiester bond between...
Abstract Background To investigate the prevalence and evolution of Porcine Reproductive Respiratory Syndrome Virus (PRRSV) at commercial fattening pig farms, a total 1397 clinical samples were collected from single cycle seven farms in five provinces China 2020 to 2021. Results The RT‒PCR results revealed that PRRSV was present on all percentage PRRSV-positive individuals 17.54–53.33%. A 344 partial NSP2 gene sequences 334 complete ORF5 obtained positive samples. statistical showed PRRSV-2...
Porcine reproductive and respiratory syndrome virus (PRRSV) has brought serious economic losses to pig industry. PRRSV-1 have existed in China for more than 25 years. The prevalence features of on Chinese farms are unclear. We continuously monitored PRRSV a farm with strict biosafety measures Henan Province, China, 2020. results showed that multiple types coexisted this single farm. was one the main circulating strains responsible infections throughout nearly entire epidemic cycle....
Topoisomerase III-beta (Top3b) reduces nucleic acid torsional stress and intertwining generated during RNA DNA metabolism while protecting the genome from pathological R-loops, which otherwise result in breakage instability. By studying Top3b knockout mice (Top3b-KO), we find that loss of accelerates development spontaneous lymphoid tumors arising spleens lymph nodes, organs with prominent expression. Aging Top3b-KO also display splenomegaly systemic immune alterations including neutrophilia...
Porcine reproductive and respiratory syndrome virus 1 (PRRSV-1) is one of the main pathogens causing porcine (PRRS). In recent years, rate PRRSV-1 detection in China has gradually increased, strains reported belong to subtype I (Global; Clade A-L). present study, a novel strain, TZJ2134, was found during epidemiological surveillance Shandong Province China. We obtained two fragments TZJ2134 genome: TZJ2134-L12 (located at nt 1672-nt 2112 partial Nsp2 gene) TZJ2134-(A+B) 7463-nt 11272 Nsp9,...
// Tong-Yun Wang 1, * , Yong-Gang Liu Liang Li 1 Gang Hai-Ming Hong-Liang Zhang Shi-Fei Zhao Jia-Cong Gao Tong-Qing An Zhi-Jun Tian Yan-Dong Tang and Xue-Hui Cai State Key Laboratory of Veterinary Biotechnology, Harbin Research Institute Chinese Academy Agricultural Sciences, 150001, China These authors contributed equally to this work Correspondence to: Cai, email: cai139@hvri.ac.cn Tang, tangyandong2008@163.com Keywords: PRRSV; CD163; infection; switch; abundance Received: July 07,...
Abstract The long noncoding RNA urothelial carcinoma‐associated 1 (UCA1) has been reported to sustain the proliferation of acute myeloid leukemia (AML) cells through downregulating cell cycle regulators p27 kip1 . Yet, foundational mechanism UCA1 in AML pathologies remains unclear. Herein, we found an escalation expression and suppression miR‐204 pediatric patients cells. silencing suppressed proliferative abilities, promoted apoptotic rates, decreased Ki67, increased cleaved caspase‐3...
The newly emerged sublineage 1.5 (NADC34-like) porcine reproductive and respiratory syndrome virus (PRRSV) has posed a direct threat to the Chinese pig industry since 2018. However, prevalence impact of NADC34-like PRRSV on farms is unclear. In present study, we continuously monitored pathogens-including PRRSV, African swine fever (ASFV), classical (CSFV), pseudorabies (PRV), circovirus 2 (PCV2)-on fattening farm with strict biosecurity practices located in Heilongjiang Province, China, from...
Classical swine fever (CSF) is one of the most devastating and highly contagious viral diseases in world. Since late 2014, outbreaks a new sub-genotype 2.1d CSF virus (CSFV) had caused substantial economic losses numbers C-strain vaccinated farms China. The objective present study was to explore genomic characteristics pathogenicity newly emerged CSFV isolates China during 2014-2015.All 8 belonged genetic 2.1d. Some variations or deletions were found UTRs E2 these isolates. In addition, HLJ1...
Although IGF1 is important for the proliferation and differentiation of chondrocytes, its underlying molecular mechanism still unknown. Here we addressed physiologic function in antler cartilage explored interplay IGF1, IRS1/2 RUNX1 chondrocyte differentiation. The results showed that was highly expressed chondrocytes. Exogenous rIGF1 could increase chondrocytes cell proportion S phase, whereas IGF1R inhibitor PQ401 abrogated induction by rIGF1. Simultaneously, stimulate expression IHH which...
Osteosarcoma (OS) is a malignant mesenchymal tissue tumor known to occur in children and adolescents, pulmonary metastasis often leads death these patients. The mechanism underlying OS progression remains unclear. Therefore, identifying new therapeutic targets treatment modalities for urgently needed. Abnormally expressed non-coding circular RNAs (circRNAs) are crucial the occurrence development of OS. purpose this study was explore expression role novel circRNA circ_000203 elucidate...
The objective of the present study was to investigate potential role immunization against INH on testicular development, serum reproductive hormone (FSH, LH and T) concentrations, biochemical indexes (GGT, ALP, ACP, LDH, α-neutral glucosidase carnitine) cell apoptosis in rats. Forty healthy male rats were randomly divided into four groups (n = 10 per group), respectively immunized with 0.5 mL C500/pVAX-asd-IS-L (108 CFU/ mL), C500/pVAX-asd-IS-M (109 C500/pVAX-asd-IS-H (1010 mL) or C500...
A latex microsphere-based immunochromatographic strip (ICS) test was successfully developed for the rapid and sensitive detection of porcine reproductive respiratory syndrome virus (PRRSV). The PRRSV N protein-specific monoclonal antibody (mAb) 1H4 labeled with microspheres dispensed on a conjugate pad use as detector. same mAb goat anti-mouse were blotted nitrocellulose membrane to generate control lines, respectively. limit approximately 5 × 102.0 median tissue culture infectious dose...
Abstract Long noncoding RNA (lncRNA) growth arrest‐specific 5 (GAS5) is implicated in several cancers via modulating microRNAs (miRs). However, little information available about the correlation between GAS5 and miR‐10b. Therefore, we sought out to investigate biological role of GAS5‐miR‐10b node mainly glioma cells. We artificially modulated explore its roles viability assayed by cell counting kit‐8 (CCK‐8), motile activities 24‐Transwell assay, as well apoptosis a flow cytometer Western...
Porcine reproductive and respiratory syndrome virus (PRRSV) causes tremendous economic losses to the swine industry worldwide. In China, novel PRRSVs have frequently emerged in recent years, but evolutionary relationship among these viruses has remained unclear. present study, a 4-year PRRSV genome-monitoring study was performed on samples from pig farm. We observed that NADC30-like with higher mutation rates replaced HP-PRRSVs as epidemic strains. monitored variation same strain evolved...
This study was conducted to investigate the effects of a traditional Chinese herbal medicine complex (TCHMC) on productive performance periparturient dairy cows. Eighteen non-lactating pregnant Holstein cows with similar body conditions 1 2 parity were randomly divided into three groups (n = 6), receiving basal diet 0 (CON group), 200 (T-200 and 300 (T-300 group) g TCHMC per day from 14 9 days prepartum. The results demonstrated that treatments decreased gestation, calving first service,...
In 2014-2015, the epidemic of classical swine fever (CSF) occurred in many large-scale pig farms different provinces China, and a subgenotype 2.1d CSF virus (CSFV) was newly identified.The phylogenetic relationship, genetic diversity, status 2014-2015 CSFV isolates, 18 new isolates collected 2015, 43 other strains isolated were fully analysed, together with 163 reference isolates.Fifty-two belonged to nine 2.1b. The two showed unique molecular characteristics. Furthermore, found possibly...