A5076098843- Plant Virus Research Studies
- Animal Disease Management and Epidemiology
- Viral Infections and Immunology Research
- Insect-Plant Interactions and Control
- Insect symbiosis and bacterial influences
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Plant Pathogenic Bacteria Studies
- Vector-Borne Animal Diseases
- Research in Cotton Cultivation
- Plant Disease Resistance and Genetics
- Plant and Fungal Interactions Research
- Viral Infectious Diseases and Gene Expression in Insects
- Virus-based gene therapy research
- CRISPR and Genetic Engineering
- Plant Parasitism and Resistance
- Plant Genetic and Mutation Studies
- Mosquito-borne diseases and control
- Plant Molecular Biology Research
- Plant-Microbe Interactions and Immunity
- Cocoa and Sweet Potato Agronomy
- Phytoplasmas and Hemiptera pathogens
- Plant Gene Expression Analysis
- Invertebrate Immune Response Mechanisms
- Aquaculture disease management and microbiota
South China Agricultural University
2016-2025
Chinese Academy of Agricultural Sciences
2016-2025
Harbin Veterinary Research Institute
2016-2025
Xinjiang Agricultural University
2023-2024
Recently, CRISPR-Cas (clustered, regularly interspaced short palindromic repeats-CRISPR-associated proteins) system has been used to produce plants resistant DNA virus infections. However, there is no RNA control method in that uses target the viral genome directly. Here, we reprogrammed CRISPR-Cas9 from Francisella novicida confer molecular immunity against viruses Nicotiana benthamiana and Arabidopsis plants. Plants expressing FnCas9 sgRNA specific for cucumber mosaic (CMV) or tobacco...
A novel dwarf and twisting syndrome first observed on rice in Nghe An Province, Vietnam, 2009 has spread rapidly to the other 19 provinces of North Central Vietnam. Infected plants showed stunting, darkening leaves, leaf tips, splitting margins. At a later stage, white waxy enations that eventually turned black were underside blades, sheaths, culms. The disease also infected maize after was harvested. stunted dark green with small along minor veins back leaves. agent now been identified as...
Abstract Background To investigate the prevalence and evolution of Porcine Reproductive Respiratory Syndrome Virus (PRRSV) at commercial fattening pig farms, a total 1397 clinical samples were collected from single cycle seven farms in five provinces China 2020 to 2021. Results The RT‒PCR results revealed that PRRSV was present on all percentage PRRSV-positive individuals 17.54–53.33%. A 344 partial NSP2 gene sequences 334 complete ORF5 obtained positive samples. statistical showed PRRSV-2...
In recent years, Southern rice black-streaked dwarf virus (SRBSDV), a tentative species in the genus Fijivirus (family Reoviridae), has spread rapidly and caused serious losses eastern southeastern Asia. With this spread, Rice ragged stunt (RRSV, Oryzavirus, family Reoviridae) became more common southern China, usually co-infection with former. SRBSDV RRSV are transmitted by two different of planthoppers, white-backed planthopper (WBPH, Sogatella furcifera) brown (BPH, Nilaparvata lugens),...
A new rice viral disease exhibiting distinct symptoms—yellow stripes, mosaic and twisted tips on leaves—was found in China. Electron microscopy of infected leaf cells revealed the presence bacilliform virions electron-translucent granular-fibrillar viroplasm cytoplasm. The enveloped particles were 300 to 375 nm long 45 55 wide. leafhopper Recilia dorsalis was able transmit virus seedlings, which subsequently exhibited symptoms similar those observed fields. complete genome obtained by...
Sogatella furcifera is an important phloem sap-sucking and plant virus-transmitting migratory insect of rice. Because its high reproductive potential, dispersal capability transmission viral diseases, S. causes considerable damage to rice grain production has great economical agricultural impacts. Comprehensive studies into ecological aspects virus-host interactions have been limited because the lack a well-assembled genome sequence.A total 241.3 Gb raw reads from whole were generated by...
Macroautophagy/autophagy has been recognized as a central antiviral defense mechanism in plant, which involves complex interactions between viral proteins and host factors. Rhabdoviruses are single-stranded RNA viruses, the infection causes serious harm to public health, livestock, crop production. However, little is known about role of autophagy against rhabdovirus by plant. In this work, we showed that
ABSTRACT Mutagenic nucleoside analogues can be used to isolate RNA virus high-fidelity RNA-dependent polymerase (RdRp) variants, the majority of which are attenuated in vivo . However, foot-and-mouth disease (FMDV) RdRp variants have not been isolated, and correlations between fidelity virulence remain unclear. Here, mutagen ribavirin was select a ribavirin-resistant population FMDV, 4 amino acid substitutions (D5N, A38V, M194I, M296V) were identified RdRp-coding region population. Through...
Abstract Background Southern rice black-streaked dwarf virus (SRBSDV) threatens production in China and Vietnam. The is vectored by the migrating white-backed planthopper (WBPH, Sogatella furcifera ) a circulative, propagative, persistent manner. A persistently-transmitted plant might affect its vector’s development fecundity directly infecting vector itself and/or indirectly altering host plant. This study evaluated direct indirect effects of SRBSDV on WBPH performance to better understand...
Rice gall dwarf virus (RGDV), a plant reovirus that threatens rice production in Southeast Asia and Southern China, is transmitted by the leafhopper vector Recilia dorsalis persistent-propagative manner. To assess direct effects of RGDV on R. dorsalis, we established an infected population from eggs laid viruliferous females using water-soaked filter paper culture method. Life history parameters indicated was harmful to its terms all biotic indices, including reduced survival rate, emergence...
Porcine reproductive and respiratory syndrome virus (PRRSV) has brought serious economic losses to pig industry. PRRSV-1 have existed in China for more than 25 years. The prevalence features of on Chinese farms are unclear. We continuously monitored PRRSV a farm with strict biosafety measures Henan Province, China, 2020. results showed that multiple types coexisted this single farm. was one the main circulating strains responsible infections throughout nearly entire epidemic cycle....
Senecavirus A (SVA) is a reemerging virus, and recent evidence has emphasized the importance of SVA recombination
Cap-independent translation initiation on picornavirus mRNAs is mediated by an internal ribosomal entry site (IRES) in the 5' untranslated region. The regulation of requires interaction IRES-transacting factors (ITAFs) with IRES. In this study, we identified a novel ITAF, heterogeneous nuclear ribonucleoprotein K (hnRNP K), which negatively regulates foot-and-mouth disease virus (FMDV) and viral replication. Further investigation revealed that KH2 KH3 domains hnRNP directly bind to II, III,...
The domain of unknown function 668 (DUF668) is a gene family that plays vital role in responses to adversity coercion stresses plant. However, the DUF668 not fully understood sweet potato. In this study, bioinformatics methods were used analyze number, physicochemical properties, evolution, structure, and promoter cis-acting elements IbDUF668 genes, RNA-seq qRT–PCR performed detect expression their regulation under hormonal abiotic stress. A total 14 proteins identified potato, distributed...
Porcine reproductive and respiratory syndrome (PRRS) poses a serious threat to the Chinese swine industry. The etiological agent PRRSV can be classified as either PRRSV‐1 or PRRSV‐2. Recent studies have revealed an increase in rates of detection wider distribution. However, genome China has yet fully characterized. In this study, 24 whole genomes from different farms were assembled subjected whole‐genome analysis. A phylogenetic analysis based on complete ORF5 sequences that strains belonged...
Multiple porcine reproductive and respiratory syndrome virus (PRRSV) subtypes coinfect numerous pig farms in China, commercial PRRSV vaccines offer limited cross-protection against heterologous strains. Our previous research confirmed that a lineage 1 branch attenuated live vaccine (SD-R) provides HP-PRRSV, NADC30-like NADC34-like PRRSV. HP-PRRSV has undergone significant genetic variation following nearly two decades of evolution transformed into subtype referred to as HP-like PRRSV, which...