A5071994160- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- Cancer-related molecular mechanisms research
- Genetic Syndromes and Imprinting
- Renal and related cancers
- Pluripotent Stem Cells Research
- RNA modifications and cancer
- Circular RNAs in diseases
- Gestational Diabetes Research and Management
- Xenotransplantation and immune response
- Reproductive Health and Technologies
- Prenatal Screening and Diagnostics
- Metabolism and Genetic Disorders
- Birth, Development, and Health
- CRISPR and Genetic Engineering
- Alkaloids: synthesis and pharmacology
- CAR-T cell therapy research
- Lipid metabolism and biosynthesis
- Plant-derived Lignans Synthesis and Bioactivity
- Diabetes and associated disorders
- Sperm and Testicular Function
- Virus-based gene therapy research
- Natural Compound Pharmacology Studies
Southern Medical University Shenzhen Hospital
2024
Shenzhen University
2024
First Hospital of Jilin University
2017-2022
Jilin University
2016-2022
First Bethune Hospital of Jilin University
2018
Abstract Objectives Obesity has become a common health concern around the world. Maternal obesity could cause poor reproductive outcomes due to chronic ovarian inflammation and decreased oocyte quality. However, strategies improve of obese females have not been fully studied. In this study, we aimed explore effects underlying mechanisms nicotinamide mononucleotide (NMN) on quality performance mice. Materials Methods The mouse model was established by feeding high‐fat diet which confirmed...
As one of the most important factors influencing meat quality, intramuscular fat (IMF) is regulated by genetics, nutrition and environment. However, epigenetic molecular mechanism underlying poultry IMF deposition remains poorly understood. In present study, we focused on DNA methylation, which usually regulate genes expression in post-transcription level. To investigate crucial role methylation deposition, chicken preadipocytes were isolated cultured vitro, a model adipocyte differentiation...
The type and pattern of epigenetic modification in donor cells can significantly affect the developmental competency somatic cell nuclear transfer (SCNT) embryos. Here, we investigated capacity, gene expression, modifications SCNT embryos derived from porcine bone marrow‐derived mesenchymal stem (BMSCs) fetal fibroblasts (FFs) compared to obtained vitro fertilization (IVF). Compared FFs, BMSCs had more active markers (Histone H3 modifications: H3K9Ac, H3K4me3, H3K4me2) fewer repressive...
Immunodeficient mice injected with human cancer cell lines have been used for oncology studies and anti-cancer drug trials several decades. However, rodents are not ideal species modelling because physiologically dissimilar to humans. Therefore, anti-tumour drugs tested effective in a failure rate of 90% or higher phase III clinical trials. Pigs similar humans size, anatomy, physiology metabolism rate, rendering them desirable pre-clinical animal model assessing drugs. xenogeneic immune...
Mice with humanized livers are important models to study drug toxicology testing, development of hepatitis virus treatments, and hepatocyte transplantation therapy. However, the huge difference between mouse human in size anatomy limited application mice investigating diseases. Therefore, it is urgent construct pigs precisely investigate regeneration CRISPR/Cas9 system somatic cell cloning technology were used generate two pig FAH deficiency exhibiting severe immunodeficiency (FAH/RAG1...
DNA methylation and histone modification play important roles in the development of mammalian embryos. Cytochalasin B (CB) is an actin polymerization inhibitor that can significantly affect cell activity often used studies concerning cytology. In recent years, CB also commonly being vitro experiments on embryos, but few have addressed effect epigenetic embryonic development, mechanism underlying this process unknown. This study was conducted to investigate effects parthenogenetically...
Resveratrol is a polyphenol with antiaging and anticancer effects. Most previous studies used single analysis to determine the key functions of resveratrol in inhibiting cancer progression. However, most signal transmission pathways biological processes are multilevel. We bioinformatics elucidate mechanism inhibition breast development. The mRNA expression profile GSE25412 from National Center for Biotechnology Information (NCBI) microRNA (miRNA) PubMed identifier (PMID) 26890143 were...
Wilms’ tumour 1 gene (WT1) is essential for the development of mammalian urogenital system. However, expression pattern WT1 in porcine organs still unclear. Here, we examined mRNA and protein kidneys, ovaries testes from embryonic days 35 60 (E35d, E60d, n = 3) to newborn (0d, 4) adult (210d, stages, using real-time PCR immunofluorescent staining. Real-time analysis showed that all expressed high level mRNAs, especially (p < 0.05 or 0.01 vs. kidney ovary, respectively). Morphologically,...