A5041862555- Ubiquitin and proteasome pathways
- Endoplasmic Reticulum Stress and Disease
- Enzyme Catalysis and Immobilization
- Enzyme Structure and Function
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Cancer-related gene regulation
- Microbial Metabolic Engineering and Bioproduction
- Metabolism and Genetic Disorders
- Glycosylation and Glycoproteins Research
- Genetics and Neurodevelopmental Disorders
- Cellular transport and secretion
- Cancer, Hypoxia, and Metabolism
- Blood Coagulation and Thrombosis Mechanisms
- Mast cells and histamine
- Biochemical and Molecular Research
- Carbohydrate Chemistry and Synthesis
- Vitamin K Research Studies
- Cannabis and Cannabinoid Research
- Enzyme Production and Characterization
- Amino Acid Enzymes and Metabolism
- Epigenetics and DNA Methylation
- Monoclonal and Polyclonal Antibodies Research
- Cancer Research and Treatments
- DNA Repair Mechanisms
- Cancer-related Molecular Pathways
University of Nottingham
2013-2023
Stamatopoulos and Associates (Greece)
2018
Graz University of Technology
2009
Institute of Molecular Biotechnology
2009
Imperial College London
2004-2007
Institute of Structural and Molecular Biology
2006
Centre National de la Recherche Scientifique
2005
Laboratoire de Chimie Organique
2005
Wolfson Foundation
2004
University of Graz
2001-2003
Metabolic networks are interconnected and influence diverse cellular processes. The protein-metabolite interactions that mediate these frequently low affinity challenging to systematically discover. We developed mass spectrometry integrated with equilibrium dialysis for the discovery of allostery (MIDAS) identify such interactions. Analysis 33 enzymes from human carbohydrate metabolism identified 830 interactions, including known regulators, substrates, products as well previously unreported...
Abstract Histone tail modifications control many nuclear processes by dictating the dynamic exchange of regulatory proteins on chromatin. Here we report novel insights into histone H3 structure in complex with double PHD finger (DPF) lysine acetyltransferase MOZ/MYST3/KAT6A. In addition to sampling and H4 modification status, show that DPF cooperates MYST domain promote H3K9 H3K14 acetylation, although not if H3K4 is trimethylated. Four crystal structures an extended alone unmodified or...
A custom-made enzyme: The gene and protein sequence of a new (R)-hydroxynitrile lyase from Prunus amygdalus was cloned engineered for recombinant production on large scale. enzyme is stable active at low pH values. rationally designed active-site mutant now permits the rapid stereoselective synthesis sterically hindered cyanohydrins such as ortho-chloromandelonitrile (see picture). Supporting information this article available WWW under...
Monoacylglycerol lipases (MGLs) play an important role in lipid catabolism across all kingdoms of life by catalyzing the release free fatty acids from monoacylglycerols. The three-dimensional structures human and a bacterial MGL were determined only recently as first members this lipase family. In addition to α/β-hydrolase core, they showed unexpected structural similarities even cap region. Nevertheless, basis for substrate binding conformational changes MGLs is poorly understood. Here, we...
Monoacylglycerol lipases (MGLs) catalyse the hydrolysis of monoacylglycerol into free fatty acid and glycerol. MGLs have been identified throughout all genera life adopted different substrate specificities depending on their physiological role. In humans, MGL plays an integral part in lipid metabolism affecting energy homeostasis, signalling processes cancer cell progression. bacteria, degrade short-chain monoacylglycerols which are otherwise toxic to organism. We report crystal structures...
The ubiquitin specific protease 11 (USP11) is implicated in DNA repair, viral RNA replication, and TGFβ signaling. We report the first characterization of USP11 domain architecture its role regulating enzymatic activity. consists an N-terminal "domain present USPs" (DUSP) "ubiquitin-like" (UBL) domain, together referred to as DU domains, catalytic harboring a second UBL domain. Crystal structures domains show tandem arrangement with shortened β-hairpin at two-domain interface altered surface...
Abstract Cross-talk between distinct protein post-translational modifications is critical for an effective DNA damage response. Arginine methylation plays important role in maintaining genome stability, but how this modification integrates with other enzymatic activities largely unknown. Here, we identify the deubiquitylating enzyme USP11 as a previously uncharacterised PRMT1 substrate, and demonstrate that of promotes end-resection repair double strand breaks (DSB) by homologous...
Essentials Zymogen PK is activated to PKa and cleaves substrates kininogen FXII contributing bradykinin generation. Monomeric dimeric homologue FXI utilize the N-terminal apple domains recruit substrates. A high-resolution 1.3 Å structure of full-length reveals an active conformation protease domains. The four-apple domain disc organization 180° rotated compared FXI. SUMMARY: Background Plasma prekallikrein (PK) factor XI (FXI) are domain-containing serine proteases that when FXIa cleave...
In a large number of plant species hydroxynitrile lyases catalyze the decomposition cyanohydrins in order to generate hydrogen cyanide upon tissue damage. Hydrogen serves as deterrent against herbivores and fungi. vitro are proficient biocatalysts for stereospecific synthesis cyanohydrins. Curiously, from different completely unrelated structure substrate specificity despite catalyzing same reaction. The lyase almond shows close resemblance flavoproteins glucose-methanol-choline...
Lactate dehydrogenase A (LDH-A) is a key enzyme in anaerobic respiration that predominantly found skeletal muscle and catalyses the reversible conversion of pyruvate to lactate presence NADH. LDH-A overexpressed many tumours has therefore emerged as an attractive target for anticancer drug discovery. Crystal structures human inhibitors have been described, but currently no apo or binary NADH-bound forms are available any mammalian LDH-A. Here, structure was solved at resolution 2.1 Å space...
Transaminases are pyridoxal-5'-phosphate (PLP) binding enzymes, broadly studied for their potential industrial application. Their affinity PLP has been related to performance and operational stability while significant differences in requirements have reported, the environment of PLP-binding pocket is highly conserved. In this study, thorough analysis residue interaction network three homologous transaminases Halomonas elongata (HeTA), Chromobacterium violaceum (CvTA), Pseudomonas...