A5017919644- Enzyme Catalysis and Immobilization
- Lipid metabolism and biosynthesis
- Advanced Proteomics Techniques and Applications
- Microbial Metabolic Engineering and Bioproduction
- Adipose Tissue and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Cancer, Lipids, and Metabolism
- Enzyme Structure and Function
- Metabolomics and Mass Spectrometry Studies
- Click Chemistry and Applications
- Allergic Rhinitis and Sensitization
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Pancreatic function and diabetes
- Enzyme Production and Characterization
- Biotin and Related Studies
- Metabolism and Genetic Disorders
- Biofuel production and bioconversion
- Endoplasmic Reticulum Stress and Disease
- Amino Acid Enzymes and Metabolism
- Liver Disease Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- Mass Spectrometry Techniques and Applications
- Asthma and respiratory diseases
- Cannabis and Cannabinoid Research
- Pregnancy and preeclampsia studies
Medical University of Graz
2016-2025
TU Wien
2019-2025
University of Vienna
2022-2024
BioTechMed-Graz
2014-2023
Graz University of Technology
2001-2022
Austrian Centre of Industrial Biotechnology (Austria)
2012-2021
University of Graz
2004-2016
Government ENT Hospital
2014
Cerner (United States)
2014
Digital Proteomics (United States)
2014
Mobilization of fatty acids from triglyceride stores in adipose tissue requires lipolytic enzymes. Dysfunctional lipolysis affects energy homeostasis and may contribute to the pathogenesis obesity insulin resistance. Until now, hormone-sensitive lipase (HSL) was only enzyme known hydrolyze triglycerides mammalian tissue. Here, we report that a second enzyme, (ATGL), catalyzes initial step hydrolysis. It is interesting ATGL contains "patatin domain" common plant acyl-hydrolases. highly...
Functional impairment of HDL may contribute to the excess cardiovascular mortality experienced by patients with renal disease, but effect advanced disease on composition and function is not well understood. Here, we used mass spectrometry biochemical analyses study alterations in proteome lipid isolated from maintenance hemodialysis. We identified a significant increase amount acute phase protein serum amyloid A1, albumin, lipoprotein-associated phospholipase A2, apoC-III composing uremic...
Cardiac troponins are often elevated in patients with skeletal muscle disease who have no evidence of cardiac disease. The goal this study was to characterize troponin concentrations myopathies and derive insights regarding the source T measurements. (cTnT) I (cTnI) were determined by using high sensitivity assays 74 hereditary acquired myopathies. Patients underwent comprehensive evaluation, including 12-lead electrocardiogram, 24-h magnetic resonance imaging, coronary artery computed...
Three different pathways lead to the synthesis of phosphatidylethanolamine (PtdEtn) in yeast, one which is localized inner mitochondrial membrane. To study contribution each these pathways, we constructed a series deletion mutants combinations are blocked. Analysis their growth phenotypes revealed that minimal level PtdEtn essential for growth. On fermentable carbon sources such as glucose, endogenous ethanolaminephosphate provided by sphingolipid catabolism sufficient allow amount through...
From a screening on agar plates with bis(benzoyloxyethyl) terephthalate (3PET), Bacillus subtilis p-nitrobenzylesterase (BsEstB) was isolated and demonstrated to hydrolyze polyethyleneterephthalate (PET). PET-hydrolase active strains produced clearing zones led the release of 3PET hydrolysis products terephthalic acid (TA), benzoic (BA), 2-hydroxyethyl benzoate (HEB), mono-(2-hydroxyethyl) (MHET) in supplemented liquid cultures. The 3PET-hydrolase from non-denaturating polyacrylamide gels...
Psoriasis, a chronic inflammatory skin disease, has been linked to increased myocardial infarction and stroke. Functional impairment of HDL may contribute the excess cardiovascular mortality psoriatic patients. However, data available regarding impact psoriasis on composition function are limited. from patients healthy controls was isolated by ultracentrifugation shotgun proteomics, biochemical methods were used monitor changed composition. We observed significant reduction in apoA-I levels...
A novel esterase, PpEst, that hydrolyses the co-aromatic-aliphatic polyester poly(1,4-butylene adipate-co-terephthalate) (PBAT) was identified by proteomic screening of Pseudomonas pseudoalcaligenes secretome. PpEst induced presence PBAT in growth media and had predicted arylesterase (EC 3.1.1.2) activity. showed polyesterase activity on both whole milled film releasing terephthalic acid 4-(4-hydroxybutoxycarbonyl)benzoic while end product inhibition observed. Modelling an aromatic mimicking...
Histone deacetylase inhibition counteracts adverse left ventricular remodeling, improves pulmonary function, and reverses diastolic dysfunction.
Nanoparticles, which are exposed to biological fluids rapidly interacting with proteins and other biomolecules forming a corona. In addition dimension, charge material the distinct protein corona influences interplay of nanoparticles tissue barriers. this study we were focused on impact in situ formed human plasma transfer 80 nm polystyrene (PS-particles) across placenta. To materno-to fetal PS used ex vivo placental perfusion approach, represents an intact physiological barrier. analyze...
Adeno-associated viruses (AAV) are one of the most commonly used vehicles in gene therapies for treatment rare diseases. During AAV manufacturing process, particles with little or no genetic material co-produced alongside desired capsid containing transgene interest. Because potential adverse health effects these byproducts, they considered impurities and need to be monitored carefully. To date, analytical ultracentrifugation (AUC), transmission electron microscopy (TEM) charge-detection...
CGI-58/ABHD5 coactivates adipose triglyceride lipase (ATGL). In adipocytes, CGI-58 binds to perilipin 1A on lipid droplets under basal conditions, preventing interaction with ATGL. Upon activation of protein kinase A (PKA), is phosphorylated and rapidly disperses into the cytoplasm, enabling coactivation. Because amino acid sequence murine has a predicted PKA consensus RKYS(239)S(240), we hypothesized that phosphorylation involved in this process. We show Ser239 substrate for using...
The lysosomal endoprotease cathepsin D (CatD) is an essential player in general protein turnover and specific peptide processing. CatD-deficiency associated with neurodegenerative diseases, whereas elevated CatD levels correlate tumor malignancy cancer cell survival. Here, we show that the ortholog of budding yeast Saccharomyces cerevisiae (Pep4p) harbors a dual cytoprotective function, composed anti-apoptotic part, conferred by its proteolytic capacity, anti-necrotic which resides protein's...
The function of many protein kinases is controlled by the phosphorylation a critical tyrosine residue in activation loop. Dual specificity tyrosine-phosphorylation-regulated (DYRKs) autophosphorylate on this but phosphorylate substrates aliphatic amino acids. This study addresses mechanism dual kinase activity DYRK1A and related kinases. Tyrosine autophosphorylation occurred rapidly during vitro translation did not depend non-catalytic domains or other proteins. Expression bacteria as well...
Membrane proteins play key roles in several fundamental biological processes such as cell signalling, energy metabolism and transport. Despite the significance, these still remain an under-represented group proteomics datasets. Herein, a bottom-up approach to analyse enriched membrane fraction from Drosophila melanogaster heads using multidimensional liquid chromatography (LC) coupled with tandem-mass spectrometry (MS/MS) that relies on complete solubilisation digestion of proteins, is...
Lipotoxic cardiomyopathy in diabetic and obese patients typically encompasses increased cardiac fatty acid (FA) uptake eventually surpassing the mitochondrial oxidative capacity. Lowering FA utilization via inhibition of lipolysis represents a strategy to counteract development lipotoxic heart dysfunction. However, defective triacylglycerol (TAG) catabolism oxidation humans (and mice) carrying mutated ATGL alleles provokes dysfunction questioning therapeutic approach decrease lipolysis....
Introduction: Nasal mucus is the first line defense barrier against various pathogens including allergens. Proteins in nasal maybe used as biomarkers for diagnosis or future therapeutic strategies. Proteomics opens possibility to investigate whole human proteomes.Areas Covered: We aimed analyze existing literature on and secretions proteomic approaches especially allergic rhinitis. A PubMed/Medline search was conducted entering following keywords combinations: "nasal mucus", lavage fluid,"...