A5026795134- Radiation Therapy and Dosimetry
- DNA Repair Mechanisms
- Neuroscience and Neuropharmacology Research
- Immune cells in cancer
- Advanced Proteomics Techniques and Applications
- Carbon and Quantum Dots Applications
- Advanced Nanomaterials in Catalysis
- Nanocluster Synthesis and Applications
- Cell death mechanisms and regulation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Immune Cell Function and Interaction
- Advanced Fluorescence Microscopy Techniques
- Immune responses and vaccinations
- Kruppel-like factors research
- Protein Degradation and Inhibitors
- Food Allergy and Anaphylaxis Research
- Neurogenesis and neuroplasticity mechanisms
- Mass Spectrometry Techniques and Applications
- Memory and Neural Mechanisms
- Radiation Effects and Dosimetry
- Photoreceptor and optogenetics research
- Medical and Biological Ozone Research
- Cancer Genomics and Diagnostics
- Bee Products Chemical Analysis
- Genetics and Neurodevelopmental Disorders
University of Veterinary Medicine Vienna
2019-2023
Jožef Stefan Institute
2023
Medical University of Vienna
2015-2018
University of Vienna
2016-2017
University of Belgrade
2013-2016
Vinča Institute of Nuclear Sciences
2014
Membrane proteins play key roles in several fundamental biological processes such as cell signalling, energy metabolism and transport. Despite the significance, these still remain an under-represented group proteomics datasets. Herein, a bottom-up approach to analyse enriched membrane fraction from Drosophila melanogaster heads using multidimensional liquid chromatography (LC) coupled with tandem-mass spectrometry (MS/MS) that relies on complete solubilisation digestion of proteins, is...
Some effector CD4+ T cell subsets display cytotoxic activity, thus breaking the functional dichotomy of helper and CD8+ lymphocytes. However, molecular mechanisms regulating lymphocyte (CD4+ CTL) differentiation are poorly understood. Here we show that levels histone deacetylases 1 2 (HDAC1-HDAC2) key determinants CTL differentiation. Deletions both Hdac1 Hdac2 alleles (HDAC1cKO-HDAC2HET) in cells induced a program was controlled by IFN-γ-JAK1/2-STAT1 signaling. In vitro, activated...
Abstract Our understanding of the composition and functions splenic stromal cells remains incomplete. Here, based on analysis over 20,000 single cell transcriptomes fibroblasts, we characterized phenotypic functional heterogeneity these in healthy state during virus infection. We describe eleven transcriptionally distinct fibroblastic clusters, reassuring known subsets revealing yet unascertained amongst fibroblasts occupying diverse niches. further identify striking differences innate...
The aim of this study was to investigate effects irradiations with the therapeutic proton and carbon ion beams in two non-small cell lung cancers, CRL5876 adenocarcinoma HTB177 large carcinoma. DNA damage response dynamics, cycle regulation, death pathway activation were followed. Viability both lines lower after ions compared irradiations. cells showed higher recovery than seven days following treatments, but survival rates exposure respect protons. When analyzing distribution cells, it...
The Fibronectin Leucine-Rich Transmembrane protein 2 (FLRT2) has been implicated in several hormone -and sex-dependent physiological and pathological processes (including chondrogenesis, menarche breast cancer); is known to regulate developmental synapses formation, expressed the hippocampus, a brain structure central for learning memory. However, role of FLRT2 adult hippocampus its relevance functions remains unknown. We here used single-allele knockout (FLRT2
The Spanish slug, Arion vulgaris, is considered one of the hundred most invasive species in Central Europe. immense and very successful adaptation spreading A. vulgaris suggest that it developed highly effective mechanisms to deal with infections natural predators. Current transcriptomic proteomic studies on gastropods have been restricted mainly marine freshwater gastropods. No or study has carried out so far, current study, first database from adult specimen reported. To facilitate enable...
ABSTRACT Ionizing radiation induces DNA double strand breaks (DSBs) that trigger phosphorylation of the histone protein H2AX (γH2AX). Immunofluorescent staining visualizes formation γH2AX foci, allowing their quantification. This method, as opposed to Western blot assay and Flow cytometry, provides more accurate analysis, by showing exact position intensity fluorescent signal in each single cell. In practice there are problems quantification γH2AX. paper is based on two issues: determination...
It has been shown that angiogenesis and inflammation play an important role in development of most hematological malignancies including the myeloproliferative neoplasm (MPN). The aim this study was to investigate correlate levels key angiogenic molecules such as hypoxia‐inducible factor‐1α (HIF‐1α), vascular endothelial growth factor (VEGF) nitric oxide synthase (eNOS) peripheral blood bone marrow cells MPN patients, along with JAK2 V617F mutation allele burden effects therapy. HIF‐1α VEGF...
This study characterizes the effects of sustained dark-chocolate consumption on mouse behavior and reveals its potential to induce seizure-like events in hippocampus, a brain structure critical for memory storage.
Abstract BACKGROUND Group 1 and group 2 allergens from house dust mite are the major elicitors of respiratory allergic diseases main candidates for immunotherapy. RESULTS The potential therapeutic role a chimera composed recombinant Der p ( D2 ) linked to Influenza A virus hemagglutinin H1 intranasal application was created, expressed tested in mouse model. H1D2 were produced by genetic engineering Escherichia coli their primary structure confirmed mass fingerprint. Both antigens preserved...
Molecular targeted cancer therapy is a promising treatment strategy. Considering the central role of epidermal growth factor receptor in cell proliferation and survival, there are indications that agents like tyrosine kinase inhibitors, i. e., erlotinib, may enhance antitumor by radiation. The aim this study to analyze inactivation effects g-rays test radiosensitizing potential erlotinib on human lung adenocarcinoma cells vitro. Irradiations were performed with doses ranging from 1 Gy 8 Gy....
<title>Abstract</title> Carbon quantum dots (CQDs) are well known as a promising therapeutic agent due to their excellent pro-oxidant, antioxidant, antibacterial and anticancer properties under visible light irradiation. This material is easy produce by several bottom-up or top-down methods. In this study, we used citric acid starting precursor synthesize aqueous carbon dispersion pyrolysis at 210 <sup>o</sup>C. Atomic force microscopy XPS analyses revealed that CQDs synthesized...
The aim of this study is to investigate the behavior radio-resistant human malignant cells, thus enabling better understanding radiobiological effects ions in such a case. Radiation sources as accelerated continuous ion beams and laser technology-based ultra short radiation with energy around 10 MeV will be used. HTB140 melanoma cells are chosen since it has been shown that they represent limit case cellular radio-resistance among studied tumor cell lines. These particularly interesting...
Our understanding of splenic stromal cell composition and function remains incomplete. Here, we uncover that red pulp stroma is phenotypically Ly6C+CD45+, due to acquisition hematopoietic-derived CD45 via intercellular transfer in situ. Using a rectified gating strategy integrate with surface expression, transcriptionally profiled the entire mesenchymal compartment single-cell resolution at steady state upon viral infection. We show extensive functional heterogeneity white pulp-, pulp-...