A randomized, double-blind, placebo-controlled study was conducted to evaluate if Lactobacillus helveticus Lafti L10 (Lallemand Health Solutions, Montreal, Que., Canada) supplementation during 14 weeks in winter can influence the duration, severity, and incidence of upper respiratory tract illness (URTI), as well monitor different immune parameters population elite athletes. Before after treatment, cardiopulmonary testing self-rated state moods evaluation (by Profile Mood States...

Tuberculosis (TB) remains among the most deadly diseases in world. The only available vaccine against tuberculosis is bacille Calmette-Guérin (BCG) vaccine, which does not ensure full protection adults. There a global urgency for development of an effective preventing disease transmission, and it requires novel approaches. We are exploring use lactic acid bacteria (LAB) as vector antigen delivery to mucosal sites. Here, we demonstrate successful expression surface display Mycobacterium...

Two<italic>Lactobacillus</italic>strains were evaluated for their effects on high fat diet induced pathology in mice.

Obesity is often accompanied by low-grade chronic inflammation and metabolic syndrome. It has been established that microbiota influences many physiological processes, including the development of obesity, dysbiosis observed in obese individuals. In this study, we aimed to evaluate impact a new probiotic formulation, containing two strains bioactive compound octacosanol, on body weight, parameters, concentrations certain adipocytokines appetite-regulating hormones women. This double blind...

Background/Objectives: The prevalence of obesity worldwide has rapidly increased. Numerous studies showed a beneficial effect probiotics in obese individuals, and changes hematological parameters are observed obesity. Therefore, the aim this study was to investigate novel probiotic approach on red blood cells (RBCs) platelets. Methods: Twenty-five women participated randomized, placebo-controlled were divided into experimental group (one capsule daily containing Lactiplantibacillus plantarum...

Abstract Michalickova, DM, Kostic-Vucicevic, MM, Vukasinovic-Vesic, MD, Stojmenovic, TB, Dikic, NV, Andjelkovic, MS, Djordjevic, BI, Tanaskovic, BP, and Minic, RD. Lactobacillus helveticus Lafti L10 supplementation modulates mucosal humoral immunity in elite athletes: a randomized, double-blind, placebo-controlled trial. J Strength Cond Res 31(1): 62–70, 2017—To test the influence of probiotic on immune response, trial was conducted. Thirty athletes (24 males 6 females, females: V̇ o 2 max...

Western life style, and high calorie diet in particular is causing major health problems such as insulin resistance, hepatic steatosis heart disease the modern age. High fat (HFD) induces similar changes mice, increased body weight, hypercholesterolemia accumulation of triglycerides liver. These can be ameliorated by administration some Lactobacillus species. The focus this study was to analyze fatty acid content liver, brain tissues mice fed HFD administered with either plantarum WCFS1 or...

The predictors of intestinal carriage vancomycin-resistant Enterococcus spp. (VRE) among high-risk patients in the counties Southeast Europe Region are insufficiently investigated, yet they could be key importance infection control. aim study was to identify risk factors associated with fecal VRE colonization inpatients university hospitals Serbia. comprised 268 from three hospitals. Data on patient demographics and clinical characteristics, length hospital stay, therapy, procedures were...

Recent reports suggest that the metabolic activity of enteric microbiota may influence fatty acid composition host tissue. There are many studies dealing with lactobacilli on various pathological conditions, and some effects strain-specific. This study was designed to test a particular Lactobacillus strain, rhamnosus LA68 liver brain C57BL/6 mice in absence an underlying condition. Female were supplemented live L. bacteria for duration 1 month. Serum biochemistry analyzed assessed by...

Fluorescently labeled lectins are useful tools for in vivo and vitro studies of the structure function tissues various pathogens such as viruses, bacteria, fungi. For evaluation high-mannose glycans present on glycoproteins, a three-dimensional (3D) model chimera was designed from crystal structures recombinant banana lectin (BanLec, Protein Data Bank entry (PDB): 5EXG) an enhanced green fluorescent protein (eGFP, PDB 4EUL) by applying molecular modeling mechanics expressed Escherichia coli....

Alternative in vitro tests that can be used instead of animal experiments are those most closely evaluate the biological activity drug interest. For testing potency antivenom, these methods to assess cytotoxicity. The aim this study was commonly cytotoxicity for determining protective antivenom Viekvin, which neutralizes Vipera ammodytes venom.

Abstract During time, both professionals and general public became aware of the importance animal welfare. This term not only covers endangered wild species, animals used in food industry, pets experimental animals, but also production biologics. The implementation 3R concept (Replacement, Reduction Refinement) is especially important this type production. In article, we describe for first time low dose, volume multi-site immunization protocol, as well appropriate ELISA developed European...

Abstract Michalickova, D, Minic, R, Kotur-Stevuljevic, J, Andjelkovic, M, Dikic, N, Kostic-Vucicevic, Slanar, O, and Djordjevic, B. Changes in parameters of oxidative stress, immunity, behavior endurance athletes during a preparation period winter. J Strength Cond Res 34(10): 2965–2973, 2020—The current study monitored markers immunological status 9 male elite athletes: V̇ o 2 max: 68 ± 11 ml·kg −1 ·min , age: 24 2.5 years, training loads: 128 21 metabolic equivalents-h·wk 3-month winter...

Abstract Background The screening for intestinal carriage of vancomycin-resistant Enterococcus spp. (VRE) among high risk patients in the Balkan region and molecular epidemiology VRE is insufficiently investigated, yet it could be key importance infection control. aim this study was to provide baseline data on high-risk Serbian university hospitals, determine phenotypic/genotypic profiles isolated VRE, obtain knowledge local resistance patterns bridge gaps current surveillance. Methods...

The aim of this study was to determine any differences in oxidative stress and inflammation parameters COPD patients treated with either N-acetyl cysteine (NAC) alone or NAC combination propolis (NACP).Forty the exacerbation phase were enrolled into (NAC group; n=20) NACP (NACP twice daily for one month. Redox status determined by measuring superoxide anion (O2.-), advanced oxidation protein products (AOPP), total (TOS), prooxidative-antioxidant balance (PAB), malondialdehyde (MDA), ischemia...

The goal of this work was to elucidate similarities between microorganisms from the perspective humoral immune system reactivity in professional athletes. serum IgG 14 young, individuals analyzed 23 selected as antigens by use house ELISA. Serum IgM and IgA also a control group sex age matched used for comparison. obtained absorbance levels were string values correlate different microorganisms. found be most cross reactive antibody class, Pearson's r = 0.7–0.92, very distant bacterial...