A5053111848- Mitochondrial Function and Pathology
- Nitric Oxide and Endothelin Effects
- Adipose Tissue and Metabolism
- ATP Synthase and ATPases Research
- Ion channel regulation and function
- Endoplasmic Reticulum Stress and Disease
- Pancreatic function and diabetes
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Cancer, Hypoxia, and Metabolism
- Eicosanoids and Hypertension Pharmacology
- Lipid metabolism and biosynthesis
- Metabolism and Genetic Disorders
- Metabolism, Diabetes, and Cancer
- Ion Channels and Receptors
- Autophagy in Disease and Therapy
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cannabis and Cannabinoid Research
- Protein Kinase Regulation and GTPase Signaling
- Renin-Angiotensin System Studies
- Cardiac electrophysiology and arrhythmias
- Cardiac Ischemia and Reperfusion
- Cellular transport and secretion
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Advanced Glycation End Products research
Medical University of Graz
2016-2025
BioTechMed-Graz
2017-2025
University of Graz
2001-2020
Kawasaki Heavy Industries (Japan)
2016
University of Dubrovnik
2013
Innsbruck Medical University
2009
Donauspital
2009
Institute of Molecular Biology
2004-2008
Heidelberg University
2008
University of Vienna
2002
Trophoblast invasion of the uterine extracellular matrix, a critical process human implantation and essential for fetal development, is striking example controlled invasiveness. To identify molecules that regulate trophoblast invasion, mRNA signatures cells isolated from first trimester (high invasiveness) term placentae (no/low were compared using U95A GeneChip microarrays yielding 220 invasion/migration-related genes. In this 'invasion cluster', KiSS-1 its G-protein-coupled receptor...
To analyze the functional consequences of coassembly transient receptor potential 1 (Trp1) and Trp3 channel proteins, we characterized membrane conductances divalent cation entry derived by separate overexpression coexpression both Trp isoforms. Trp1 expression generated a 1-oleoyl-2-acetyl-<i>sn</i>-glycerol (OAG)-activated conductance that was detectable only in Ca<sup>2+</sup>-free extracellular solution. gave rise to an OAG-activated suppressed but clearly at physiological...
1. We tested the hypothesis that agonist‐stimulated Ca2+ entry, and thus formation of endothelium‐derived nitric oxide (EDNO) in vascular endothelial cells, is related to activation microsomal P450 mono‐oxygenase (P450 MO) biosynthesis 5,6‐epoxyeicosatrienoic acid (5,6‐EET). 2. Several inhibitors diminished sustained [Ca2+]i plateau response agonist or intracellular store depletion with ATPase by 31‐69% (fura‐2 technique). Mn2+ influx stimulated agonists was prevented inhibitors. 3....
Although the endocannabinoid anandamide is frequently described to act predominantly in cardiovascular system, molecular mechanisms of its signaling remained unclear. In human endothelial cells, two receptors for were found, which characterized as cannabinoid 1 receptor (CB1R; CNR1) and G-protein-coupled 55 (GPR55). Both trigger distinct pathways. It crucially depends on activation status integrins cascade becomes promoted upon stimulation. Under conditions inactive integrins, initiates...
Changes in intra- and extracellular potassium ion (K+) concentrations control many important cellular processes related biological functions. However, our current understanding of the spatiotemporal patterns physiological pathological K+ changes is severely limited by lack practicable detection methods. We developed K+-sensitive genetically encoded, Förster resonance energy transfer-(FRET) based probes, called GEPIIs, which enable quantitative real-time imaging dynamics. GEPIIs as purified...
Adipose triglyceride lipase (ATGL) catalyzes the first step in hydrolysis of triacylglycerol (TG) generating diacylglycerol and free fatty acids. The enzyme requires activator protein CGI-58 (or ABHD5) for full enzymatic activity. Defective ATGL function causes a recessively inherited disorder named neutral lipid storage disease that is characterized by systemic TG accumulation myopathy. In this study, we investigated functional defects associated with mutations gene cause disease. We show...
Reprogramming of metabolic pathways determines cell functions and fate. In our work, we have used organelle-targeted ATP biosensors to evaluate cellular settings with high resolution in real time. Our data indicate that mitochondria dynamically supply for glucose phosphorylation a variety cancer types. This hexokinase-dependent process seems be reversed upon the removal or other hexose sugars. further verify cells increased consumption. Similar subcellular fluxes occurred young mouse...
Distinct subcellular pH levels, especially in lysosomes and endosomes, are essential for the degradation, modification, sorting, accumulation, secretion of macromolecules. Here, we engineered a novel genetically encoded probe by fusing pH-stable cyan fluorescent protein (FP) variant, mTurquoise2, to highly pH-sensitive enhanced yellow protein, EYFP. This approach yielded ratiometric biosensor—referred as pH-Lemon—optimized live imaging distinct conditions within acidic cellular compartments....
Recently identified core proteins (MICU1, MCU, EMRE) forming the mitochondrial Ca
Article7 August 2018Open Access Source DataTransparent process RNA editing of Filamin A pre-mRNA regulates vascular contraction and diastolic blood pressure Mamta Jain Corresponding Author [email protected] Division Cell Biology, Center for Anatomy Medical University Vienna, Austria Search more papers by this author Tomer D Mann The Mina Everard Goodman Faculty Life Sciences, Bar Ilan University, Ramat-Gan, Israel Tel Aviv Sourasky Center, Aviv, Maja Stulić Shailaja P Rao Molecular Medicine,...
Abstract Nitric oxide ("Equation missing"<!-- image only, no MathML or LaTex -->) is a free radical with wide range of biological effects, but practically impossible to visualize in single cells. Here we report the development novel multicoloured fluorescent quenching-based "Equation --> probes by fusing bacteria-derived -->-binding domain close distinct protein variants. These genetically encoded probes, referred as geNOps, provide selective, specific and real-time read-out cellular...
Histone deacetylase inhibition counteracts adverse left ventricular remodeling, improves pulmonary function, and reverses diastolic dysfunction.
Resveratrol and its derivate piceatannol are known to induce cancer cell-specific cell death. While multiple mechanisms of actions have been described including the inhibition ATP synthase, changes in mitochondrial membrane potential ROS levels, exact specificity these polyphenols remain unclear. This paper is designed reveal molecular basis cancer-specific initiation death by resveratrol piceatannol.The two lines EA.hy926 HeLa, somatic short-term cultured HUVEC were used. Cell viability...
Metabolic enzymes can adapt during energy stress, but the consequences of these adaptations remain understudied. Here, we discovered that hexokinase 1 (HK1), a key glycolytic enzyme, forms rings around mitochondria stress. These HK1-rings constrict at contact sites with endoplasmic reticulum (ER) and mitochondrial dynamics protein (MiD51). prevent fission by displacing dynamin-related (Drp1) from factor (Mff) (Fis1). The disassembly restoration correlated fission. Mechanistically, identified...
Mitochondria have been found to sequester and release Ca2+ during cell stimulation with inositol 1,4,5-triphosphate-generating agonists, thereby generating subplasmalemmal microdomains of low that sustain activity capacitative entry (CCE). Procedures prevent mitochondrial uptake inhibit local buffering CCE, but it is not clear whether has transit through or remains trapped in the mitochondria. Thus, we analyzed contribution efflux on ability mitochondria buffer Ca2+, maintain facilitate...