A5068878539- Nitric Oxide and Endothelin Effects
- ATP Synthase and ATPases Research
- Analytical Chemistry and Sensors
- Mitochondrial Function and Pathology
- Endoplasmic Reticulum Stress and Disease
- Sulfur Compounds in Biology
- Cellular transport and secretion
- Cancer, Hypoxia, and Metabolism
- FOXO transcription factor regulation
- Dietary Effects on Health
- Photoreceptor and optogenetics research
- Graphene and Nanomaterials Applications
- Metabolism and Genetic Disorders
- Adipose Tissue and Metabolism
- Redox biology and oxidative stress
- Trace Elements in Health
- Genetics, Bioinformatics, and Biomedical Research
- Metabolism, Diabetes, and Cancer
- Machine Learning in Bioinformatics
- Pancreatic function and diabetes
- Cancer Research and Treatments
- Advanced biosensing and bioanalysis techniques
- Neuroscience and Neuropharmacology Research
- Receptor Mechanisms and Signaling
- Advanced Fluorescence Microscopy Techniques
Medical University of Graz
2017-2022
Distinct subcellular pH levels, especially in lysosomes and endosomes, are essential for the degradation, modification, sorting, accumulation, secretion of macromolecules. Here, we engineered a novel genetically encoded probe by fusing pH-stable cyan fluorescent protein (FP) variant, mTurquoise2, to highly pH-sensitive enhanced yellow protein, EYFP. This approach yielded ratiometric biosensor—referred as pH-Lemon—optimized live imaging distinct conditions within acidic cellular compartments....
Cancer cells voraciously consume nutrients to support their growth, exposing metabolic vulnerabilities that can be therapeutically exploited. Here, we show in hepatocellular carcinoma (HCC) cells, xenografts, and patient-derived organoids fasting improves sorafenib efficacy acts synergistically sensitize sorafenib-resistant HCC. Mechanistically, noncanonically as an inhibitor of mitochondrial respiration, causing resistant depend on glycolysis for survival. Fasting, through reduction glucose...
Mitochondrial sirtuins (Sirts) control important cellular processes related to stress. Despite their regulatory importance, however, the dynamics and subcellular distributions of Sirts remain debatable. Here, we investigate localization sirtuin 4 (Sirt4), a variant with mitochondrial targeting sequence (MTS), by expressing Sirt4 fused superfolder green fluorescent protein (Sirt4-sfGFP) in HeLa pancreatic β-cells. Super resolution fluorescence microscopy revealed trapping Sirt4-sfGFP outer...
Nitric Oxide (NO•) is a small radical, which mediates multiple important cellular functions in mammals, bacteria and plants. Despite the existence of large number methods for detecting NO• vivo vitro, real-time monitoring at single-cell level very challenging. The physiological or pathological effects are determined by actual concentration dwell time this radical. Accordingly, that allow detection highly desirable. Recently, we expanded pallet indicators introducing single fluorescent...
Mitochondrial Ca2+ uptake into the mitochondrial matrix is a well-established mechanism. However, sub-organellar kinetics remain elusive. In present work we identified novel site-specific targeting sequences for intermembrane space (IMS) and cristae lumen (CL). We used these peptides to develop green- red- biosensors targeted IMS CL. Based on their distinctive spectral properties, comparable sensitivities constructs were suitable visualize Ca2+-levels in various (sub) compartments...
One third of all human proteins are either transmembrane or soluble secretory that first target the endoplasmic reticulum (ER). These subsequently leave ER and enter Golgi apparatus via ER-Golgi intermediate vesicular structures. Live-cell imaging cargos fused to fluorescent (FPs) enables high-resolution visualization characterization transport processes. Here, we performed fluorescence time-lapse assess Ca2+ energy dependency ER-to-Golgi in living HeLa cells, a cancer cell model which has...
ABSTRACT Cancer cells voraciously consume nutrients to support their growth, exposing a metabolic vulnerability that can be therapeutically exploited. Here we show in hepatocellular carcinoma (HCC) cells, xenografts, and patient-derived HCC organoids fasting synergistically sensitize resistant sorafenib. Mechanistically, sorafenib acts non-canonically as an inhibitor of mitochondrial respiration, causing depend on glycolysis for survival. Fasting, through reduction glucose impeded...
Nitric Oxide (NO•) is a small radical, which mediates multiple important cellular functions in mammals, bacteria and plants. Despite the existence of large number methods for detecting NO• vivo vitro, real-time monitoring at single-cell level very challenging. The physiological or pathological effects are determined by actual concentration dwell time this radical. Accordingly, that allow detection highly desirable. Recently, we expanded pallet indicators introducing single fluorescent...
S-Nitrosylation of cysteine residues is an important molecular mechanism for dynamic, post-translational regulation several proteins, providing a ubiquitous redox regulation. Cys are present in fluorescent proteins (FP), including members the family Aequorea victoria Green Fluorescent Protein (GFP)-derived FPs, where two highly conserved contribute to favorable environment autocatalytic chromophore formation reaction. The effect nitric oxide on fluorescence properties FPs has not been...