Superior kidding rate is an important economic trait in production of meat goat, and ovulation the precondition rate. MicroRNAs (miRNAs) play critical roles almost all ovarian biological processes, including folliculogenesis, follicle development, atresia, luteal development regression. To find out different activity recruitment with miRNA-mediated posttranscriptional regulation, small RNAs expressed pattern tissues multiple uniparous Anhui White goats during follicular phase was analyzed...

Reproduction traits are crucial for livestock breeding and represent key economic indicators in the domestic goat (Capra hircus) industry. The oviduct, a critical organ female mammals, plays pivotal role several reproductive processes; however, its molecular mechanisms remain largely unknown. Non-coding RNA mRNAs essential regulatory elements yet their specific roles networks oviducts unclear. In this study, we conducted small sequencing of oviduct high- low-fecundity goats during follicular...

A close association exists among testicular function, gut microbiota regulation, and organismal metabolism. In this study, serum seminal plasma metabolomes, the rumen microbiome of sheep with significant differences in sperm viability, were explored. Serum metabolomes differed significantly between high-motility (HM) low-motility (LM) groups sheep, 39 differential metabolites closely related to motility found while 35 samples. 16S rRNA sequence analysis showed that relative abundance HM LM...

Abstract Allicin is a sulfur‐containing compound extracted from raw garlic ( Allium sativum L .). We compared the effect of allicin addition on growth performance, serum biochemical parameters, and rumen microbiota goats to monensin. Twenty‐four Anhui white were assigned randomly one three dietary treatments: 1) basal diet (CON); 2) with at 750 mg per head day (AC); 3) monensin 30 kg (MS). Animals fed for 8 weeks. Results showed average daily gain, feed efficiency was increased addition....

The aim of this study was to determine whether the inclusion 40% common vetch (CV) hay as a feed ingredient in place alfalfa (AH) would improve performance and ruminal fermentation microbiota fattening lambs. Twenty lambs were equally divided into two groups: control group (fed AH with 20% rice straw) CV straw). Concerning quality, had greater vitro digestibility dry matter neutral detergent fiber (

Fresh semen is most commonly used in an artificial insemination of small ruminants, because low fertility rates frozen sperm. Generally, when developing and applying assisted reproductive technologies, sheep goats are classified as one species. In order to optimize sperm cryopreservation protocols goat, differences proteomes between ram buck necessary investigate, which may contribute function spermatozoa. the current work, a data-independent acquisition-mass spectrometry proteomic approach...

Artificial directional selection has replaced natural and resulted in trait differences across breeds domestic animal breeding. However, the molecular mechanism by which oviduct regulates litter size remains largely elusive goats during follicular phase. Accumulating data have linked lncRNAs to reproductive activities; however, little is known about modulation oviduct. Herein, RNA-seq was used measure mRNA lncRNA expression levels low- high-fecundity goats. We observed distinctive terms of...

Sperm proteins play vital roles in improving sperm freezing resilience domestic animals. However, it remains poorly defined which regulate the of spermatozoa rams (Ovis aries). Here, we compared proteome ram with a high cryopreservation recovery ratio (HCR) that low (LCR) using tandem mass tag-based quantitative proteomics approach. Bioinformatic analysis was performed to evaluate differentially expressed (DEPs). A total 2464 were identified, and 184 DEPs screened. Seventy-two higher LCR...

Y-27632, as a cytoskeleton protector, is commonly used for low-temperature preservation of cells. Goat sperm are prone to damage the under conditions, leading loss vitality. However, Y-27632 small molecule has not yet been in research on goat semen. This study aims address issue low temperature-induced motility goats by using and explore regulation metabolism. At temperature 4 °C, different concentrations were added diluent. The quality temperature-preserved semen was evaluated detecting...

This study aimed to investigate the effects of different dietary concentrate-to-forage ratios on slaughter performance, meat quality, rumen fermentation, microbiota and fecal in Tibetan sheep. A total sixty male sheep were equally allocated into three groups based ratios, i.e., 30:70 (C30), 50:50 (C50), 70:30 (C70). Compared with C30 group, fed C70 diet resulted a higher (

The reproduction of sheep is affected by many factors such as light, nutrition and genetics. Hypothalamic-pituitary-gonadal (HPG) axis an important pathway for reproduction, changes in HPG axis-related gene expression can affect reproduction. In this study, a model bilateral ovarian removal estrogen supplementation (OVX + E2) was applied to screen differentially expressed genes miRNAs under different photoperiods using whole transcriptome sequencing reveal the regulatory effects photoperiod...

The mammalian oviduct is functionally highly diverse during the estrus cycle. It provides a suitable milieu for oocyte maturation, sperm capacitation, fertilization, early embryo development and transportation. While there have been many studies of molecular mechanisms on kidding number goats, systematic analysis by which underlying circular RNAs (circRNAs) changes in related to prolificacy traits lacking. Herein, we present comprehensive circRNA atlas among high- low-fecundity goats...

PIWI-interacting RNAs (piRNAs), as a novel class of small non-coding that have been shown to be indispensable in germline integrity and stem cell development. However, the expressed characteristics regulatory roles piRNAs during different reproductive phases animals remain unknown. In this study, we investigated expression profiles ovaries sheep luteal phase (LP) follicular (FP) using Solexa sequencing technique. A total 85,219 1,27,156 tags were identified ovine across two phases. Most...

The oviduct is associated with embryo development and transportation regulates the pregnancy success of mammals. Previous studies have indicated a molecular mechanism lncRNAs in gene regulation reproduction. However, little known about function modulating goat kidding numbers. Therefore, we combined RNA sequencing (RNA-seq) to map expression profiles at luteal phase from high- low-fecundity goats. results showed that 2023 differentially expressed mRNAs (DEGs) 377 (DELs) transcripts were...

Spermatozoa are unique cells that carry a library of proteins regulate the functions molecules to achieve functional capabilities. Currently, large amounts protein have been identified in spermatozoa from different species using proteomic approaches. However, proteome characteristics and regulatory mechanisms bucks versus rams not fully unraveled. In this study, we performed tandem mass tag (TMT)-labeled quantitative analysis investigate profiles buck (Capra hircus) ram (Ovis aries), two...

The time of puberty onset is crucial for female animal, as it can affect the generation interval, feeding costs and utilization animals. However, little known about mechanism hypothalamic lncRNAs (long noncoding RNAs) in regulatory goat onset. Therefore, genome-wide transcriptome analysis was performed goats to clarify roles mRNAs puberty. In present study, co-expression network differentially expressed (DE) hypothalamus identified FN1 hub gene, ECM-receptor interaction, Focal adhesion...

This study investigated the effects of three feeding systems, indoor (CONT), with time-restricted grazing artificial pasture (4 h/day, G4H), and an eight-hour daily (G8H), on growth performance, serum metabolites, ruminal fermentation, fecal microbiota composition lambs. Average gain showed a tendency (p = 0.081) to be higher for G4H group compared CONT group. Moreover, systems did not have significant effect most biochemical indicators in Concentrations glutathione peroxidase...

The aim of this study was to evaluate the effects three feeding systems, i.e., indoor (CON), with 4-h daily access grazing artificial pasture (ITGP), and 8-h (IEGP), on plasma antioxidant immunological capacity, slaughter characteristics, meat quality economic efficiency Huang-huai lambs.Thirty-three healthy rams similar body weight (approximately 5 mo age, 28.96±1.01 kg) were assigned equally experimental groups. When finished fattening, six lambs from each group collect blood samples for...