Background Many evidences show the inverse correlation between helminth infection and allergic or autoimmune diseases. Identification characterization of active helminth-derived products responsible for beneficial effects on inflammatory diseases will provide another feasible approach to treat these Methods Findings Colitis was induced in C57BL/6 mice by giving 3% DSS orally 7 days. During this period, were treated daily with excretory/secretory from T. spiralis adult worms (AES)...

Helminth infections and their components have been shown to a protective effect on autoimmune diseases. The isolated purified protein from Schisotosoma japonicum its potential therapeutic trinitrobenzene sulfonic acid (TNBS)-induced colitis could provide an alternative way treat inflammatory bowel disease (IBDs). Colitis was induced in Balb/c mice by rectal administration of 2.5 % TNBS, followed intraperitoneal injection rSjcystatin 50 μg at 6 h 24 afterwards. inflammation monitored...

Purpose This study mimics the metabolic environment of dysfunction-associated steatotic liver disease (MASLD) and diabetic mellitus (DM) to investigate function BRCA1 in regulating glucose lipid metabolism hepatocytes under high (HG) settings. Methods MASLD DM-related datasets (GSE89632, GSE95849) were screened for overlapping genes, Protein-Protein Interaction (PPI) network enrichment analyses performed. Then, quantitative real-time polymerase chain reaction (qRT-PCR), Western Blotting...

Sepsis is a life-threatening complication of an infection and remains one the leading causes mortality in surgical patients. Bacteremia induces excessive inflammatory responses that result multiple organ damage. Chronic helminth helminth-derived materials have been found to immunomodulate host immune system reduce inflammation against some allergic or diseases. Schistosoma japonicum cystatin (Sj-Cys) cysteine protease inhibitor regulatory T-cells potential immunomodulatory. The effect Sj-Cys...

Multi-organ failure caused by the inflammatory cytokine storm induced severe infection is major cause of death for sepsis. Sj-Cys a cysteine protease inhibitor secreted Schistosoma japonicum with strong immunomodulatory functions on host immune system. Our previous studies have shown that treatment recombinant protein (rSj-Cys) attenuated inflammation However, immunological mechanism underlying immunomodulation regulating diseases not yet known. In this study, we investigated effect...

Abstract Background Myocardial dysfunction is one of the most common complications multiple organ failure in septic shock and significantly increases mortality patients with sepsis. Although many studies having confirmed that helminth-derived proteins have strong immunomodulatory functions could treat inflammatory diseases, there no report on therapeutic effect Schistosoma japonicum -produced cystatin ( Sj- Cys) sepsis-induced cardiac dysfunction. Methods A model myocardial injury was...

Sepsis is a life-threateningorgandysfunction caused by the cytokine storm induced severe bacterial infection. Excessive inflammatory responses are responsible for lethal organ damage during early stage of sepsis. Helminth infection and helminth-derived proteins have been identified to ability immunomodulate host immune system reducing inflammation against diseases. Trichinella spiralis cystatin (Ts-Cys) cysteine protease inhibitor with strong immunomodulatory functions on system. Our...

Acute lung injury (ALI) is a common complication of systemic inflammation or sepsis with high morbidity and mortality. Although many studies have confirmed that helminth-derived proteins had strong immunomodulatory functions could be used to treat inflammatory diseases, there no report on the therapeutic effect excretory-secretory products Trichinella spiralis adult worms ( Ts -AES) sepsis-induced ALI. In this study, efficacy -AES ALI underlying immunological mechanism signaling pathway were...

Abstract Background The primary pathophysiological process of sepsis is to stimulate a massive release inflammatory mediators trigger systemic response syndrome (SIRS), the major cause multi-organ dysfunction and death. Like other helminths, Echinococcus granulosus induces host immunomodulation. We sought determine whether E. cyst fluid ( Eg CF) displays therapeutic effect on sepsis-induced inflammation tissue damage in mouse model. Methods anti-inflammatory effects CF were determined by...

Atherosclerosis is a chronic inflammation of the arterial vessel wall driven by lipid metabolism disorders. Although helminthic infection and their derivatives have been identified to attenuate inflammatory diseases, immunomodulatory effect recombinant Schistosoma japonicum cystatin (rSj-Cys) on metabolic diseases atherosclerosis has not reported. In this study, we investigated therapeutic efficacy rSj-Cys atherosclerotic renal damage explored related immunological mechanism. The results...

Paraquat (PQ) is a widely used herbicide that poisons human by accident or intentional ingestion. PQ poisoning causes systemic inflammatory response syndrome (SIRS) resulting in acute lung injury (ALI) with an extremely high mortality rate. Blood trematode Schistosoma japonicum-produced cystatin (Sj-Cys) strong immunomodulatory protein has been experimentally to treat inflammation related diseases. In this study, Sj-Cys recombinant (rSj-Cys) was PQ-induced and the immunological mechanism...

Abnormal osteoclastic activation and secretion of cysteine proteinases result in excessive bone resorption, which is one the primary factors development metabolic disorders, such as rheumatoid arthritis osteoporosis. Mammalian cystatins have been demonstrated to restrain resorption alleviate severe osteolytic destruction via blocking activity proteinases. However, specific effects parasite on formation function osteoclasts remain unclear. The purpose current study was explore from...

The association of anesthetic exposure in infants or young children with the long-term impairment neurologic functions has been reported previously; however, underlying mechanisms remain largely unknown. In order to identify dysregulated gene expression cognitive caused by sevoflurane at postnatal stage, present study initially performed behavioral tests on adult Wistar rats, which received 3% day 7 (P7) for different time course. Subsequently, transcriptome profiling hippocampal tissues...

Selection pressure caused by long-term intensive use of insecticides is the key driving force in resistance development. Additional parameters such as environmental conditions may affect both mosquito response to and selection mechanisms. In this context, we analyzed determinants kdr prevalence Anopheles sinensis across China. We collected frequency from 48 sites central southern China, together with factors including climatic data, topographic features, main crops, land cover types. Trend...

To understand the status of resistance to multiple insecticides and frequencies kdr mutations in Culex pipiens pallens from north-central Anhui Province.From July September, 2014, C. mosquito larvae were collected Huaibei, Bengbu Chuzhou cities Province reared adults. The female adult mosquitoes at 3-5 days post emergence tested for susceptibility four insecticides, namely 0.05% deltamethrin, 5% malathion, 0.1% bendiocarb 4% DDT, by using standard WHO tube bioassay. detection point gene...

Sepsis is a disease caused by multiple microbial infections resulting in organ failure. Schistosoma japonicum secreted cystatin (Sj-Cys) strong immunomodulator that stimulates M2 macrophages and alleviates inflammatory damage sepsis. To determine whether the therapeutic effect of Sj-Cys on sepsis can be conveyed exosomes released Sj-Cys-stimulated macrophages, RAW264.7 were stimulated with rSj-Cys vitro, obtained from cell culture supernatant ultracentrifugation. was induced BALB/c mice...

Abstract Background Sepsis is a potentially fatal systemic inflammatory response syndrome (SIRS) that threatens millions of lives worldwide. Echinococcus granulosus antigen B ( Eg AgB) protein released by the larvae tapeworm. This has been shown to play an important role in modulating host immune response. In this study we expressed AgB as soluble recombinant E. coli (r and explored its protective effect on sepsis. Methods The sepsis model was established cecal ligation puncture (CLP)...

To explore the anti-tumor effect of 17XL strains Plasmodium yoelii (P.y) infection on melanoma in mice.B16F10 tumor cells were axillarilly injected into right flank 20 C57BL/6 mice to establish tumor-bearing mouse models. The next day, randomly divided a P.y group and control group, 10 each group. Each was intraperitoneally with 1×106 red blood including 20% cells, one normal mice. time formation two groups observed volumes measured.The group［ (11.30 ± 0.21) d］was significantly later than...

To observe the effect of excretory/secretory products from Trichinella spiralis adult worms (AES) on cecal ligation and puncture (CLP) -induced sepsis in mice.Forty-eight BALB/c mice were randomly divided into 3 groups：a sham operation group (PBS + group, Group A), a CLP-induced (PBS+CLP B) an AES treatment (AES+CLP C). The each intraperitoneally injected with 25 μg or PBS only as control total volume 200 μl. Eight selected for survival analysis 96 hours. other 8 observed pathological...

Abstract Background Ischemia-induced inflammatory response is the main pathological mechanism of myocardial infarction (MI)-caused heart tissue injury. It has been known that helminths and worm-derived proteins are capable modulating host immune to suppress excessive inflammation as a survival strategy. Excretory/secretory products from Trichinella spiralis adult worms ( Ts -AES) have shown ameliorate inflammation-related diseases. In this study, -AES were used treat mice with MI determine...