A5006502427- Cancer-related molecular mechanisms research
- Cytomegalovirus and herpesvirus research
- SARS-CoV-2 and COVID-19 Research
- Psoriasis: Treatment and Pathogenesis
- Rheumatoid Arthritis Research and Therapies
- COVID-19 Clinical Research Studies
- RNA modifications and cancer
- Autoimmune and Inflammatory Disorders Research
- Immunotherapy and Immune Responses
- Immune Cell Function and Interaction
- Spondyloarthritis Studies and Treatments
- Lung Cancer Treatments and Mutations
- Sperm and Testicular Function
- Parasites and Host Interactions
- Herpesvirus Infections and Treatments
- Inflammasome and immune disorders
- Lymphoma Diagnosis and Treatment
- Ferroptosis and cancer prognosis
- Cancer, Hypoxia, and Metabolism
- Selenium in Biological Systems
- Cancer, Lipids, and Metabolism
- Vitamin K Research Studies
- Peptidase Inhibition and Analysis
- T-cell and B-cell Immunology
- Reproductive Biology and Fertility
University of Electronic Science and Technology of China
2020-2025
Changhai Hospital
2025
Second Military Medical University
2025
Northwest A&F University
2025
Chinese Academy of Agricultural Sciences
2025
Institute of Animal Sciences
2025
Dalian Medical University
2015-2025
Second Affiliated Hospital of Dalian Medical University
2015-2025
Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital
2013-2024
Second Affiliated Hospital of Zhejiang University
2022-2024
Abstract Modified Vaccinia Ankara (MVA) is a highly attenuated poxvirus vector that widely used to develop vaccines for infectious diseases and cancer. We demonstrate the construction of vaccine platform based on unique three-plasmid system efficiently generate recombinant MVA vectors from chemically synthesized DNA. In response ongoing global pandemic caused by SARS coronavirus-2 (SARS-CoV-2), we use this rapidly produce fully synthetic (sMVA) co-expressing SARS-CoV-2 spike nucleocapsid...
The human enthesis conventional T cells are poorly characterised.To study the biology of in enthesis.CD4+ and CD8+ were investigated 25 samples using immunofluorescence, cytometrically, bulk RNAseq quantitative real-time PCR following anti-CD3/CD28 bead stimulation to determine interleukin (IL)-17A tumour necrosis factor (TNF) levels. T-cell receptor (TCR) repertoires characterised a search for putative reactivity was carried out TCR3 database. impact pharmacological antagonism with retinoic...
Second-generation COVID-19 vaccines could contribute to establish protective immunity against SARS-CoV-2 and its emerging variants. We developed COH04S1, a synthetic multiantigen modified vaccinia Ankara-based vaccine that co-expresses spike nucleocapsid antigens. Here, we report COH04S1 efficacy in animal models. demonstrate intramuscular or intranasal vaccination of Syrian hamsters with induces robust Th1-biased antigen-specific humoral cross-neutralizing antibodies (NAb) protects weight...
COH04S1, a synthetic attenuated modified vaccinia virus Ankara vector co-expressing SARS-CoV-2 spike and nucleocapsid antigens, was tested for safety immunogenicity in healthy adults.This combined open-label randomised, phase 1 trial done at the City of Hope Comprehensive Cancer Center (Duarte, CA, USA). We included participants aged 18-54 years with negative antibody PCR test, normal haematology chemistry panels, electrocardiogram troponin concentration, pregnancy test if female, body-mass...
Functional loss of endothelial cells will lead to development and progression atherosclerosis in diabetic patients. However, dysfunction diabetes has yet be fully understood. We aimed characterize the potential effects liraglutide, a glucagon-like peptide-1 (GLP-1) receptor agonist, on preventing high glucose-induced excessive mitophagic response. Pretreatment with liraglutide prevented downregulation eNOS phosphorylation NO secretion, reduced apoptosis oxidative stress human umbilical vein...
Abstract Background This study was designed to assess the associations between emerging cardiometabolic indices—the atherogenic index of plasma (AIP), stress hyperglycemia ratio (SHR), triglyceride-glucose (TyG) index, and homeostasis model assessment insulin resistance (HOMA-IR)—and incidence diabetic kidney disease (DKD) in type 2 diabetes (T2D) patients. Methods We consecutively enrolled 4351 T2D The AIP, SHR, TyG HOMA-IR were calculated from baseline parameters. DKD defined as a urine...
The emergence of resistance to PARP1 inhibitors poses a current therapeutic challenge, necessitating the development novel strategies overcome this obstacle. present study describes design and synthesis series small molecules that target both c-Met. Among them, compound 16 is identified as highly potent dual inhibitor, exhibiting excellent inhibitory activities against (IC50 = 3.3 nM) c-Met 32.2 nM), well demonstrating good antiproliferative effects on HR-proficient cancer cell lines those...
Background Quercetin, a widespread polyphenolic flavonoid, is known for its extensive health benefits and commonly found in the plant kingdom. The natural occurrence extraction methods of quercetin are crucial due to bioactive potential. Purpose This review aims comprehensively cover sources quercetin, methods, bioavailability, pharmacokinetics, role various cell death pathways liver fibrosis. Methods A comprehensive literature search was performed across several electronic databases,...
Triplex vaccine was developed to enhance cytomegalovirus (CMV)-specific T cells and prevent CMV reactivation early after hematopoietic stem cell transplant (HCT).
Objective: Detection of aberrant methylated genes in feces has been developed as an early screening method for colorectal cancer.The aim this study was to probe the methylation status SEPT9, BMP3, NDRG4, and SDC2 stool whether these is associated with cancer.Materials Methods: DNAs were isolated purified from cancerous non-cancerous samples cancer tissue.Gene levels quantified by methylation-specific PCR on analyzed a diagnostic model.Results: DNA NDRG4 SDC2, but not had potential detecting...
Neutrophils are present in the early phases of spondyloarthritis-related uveitis, skin and intestinal disease, but their role enthesitis, a cardinal musculoskeletal lesion spondyloarthritis, remains unknown. We considered neutrophils experimental SKG mouse model SpA human axial entheses.Early inflammatory infiltrates peripheral entheseal sites mice were evaluated using immunohistochemistry laser capture microdissection tissue. Whole transcriptome analysis was carried out Affymetrix gene...
Rheumatoid arthritis (RA) is a prevalent autoimmune disease whose main features include chronic synovial inflammation, bone destruction, and joint degeneration.Neutrophils are often considered to be the first responders inflammation key presence in inflammatory milieu of RA.Neutrophil extracellular traps (NETs), meshwork DNA-histone complexes proteins released by activated neutrophils, widely involved pathophysiology diseases, especially RA, addition playing role neutrophil innate immune...
Identifying inflammation-induced leukocyte subsets and their derived circulating factors has been instrumental in understanding the progession of ALI/ARDS. Nevertheless, how primary non-leukocyte populations distal organs contribute to ALI/ARDS remains poorly defined. Here, we report one population erythroblast-like cells (Ter-cells) deriving from megakaryocyte- erythroid progenitor with a unique Ter-119+CD45-CD71+ phenotype Ter-cells induced by spleen are chemoattracted into lung inhibit...
Objectives SLE is a multifaceted autoimmune disorder with complex pathogenesis involving genetic, environmental and hormonal factors, which converge on immune dysregulation. The T cell receptor (TCR) repertoire’s role in has garnered significant interest due to its potential both diagnostics therapeutics. Our study aimed delineate the variances TCRβ repertoire between patients healthy individuals, correlating these differences severity subtypes of SLE. Methods We conducted an analysis blood...
Glucose oxidase (GOD) is an oxygen-consuming dehydrogenase that can catalyze the production of gluconic acid hydrogen peroxide from glucose, and its specific mechanism action makes it promising for applications, while low catalytic activity poor thermostability have become main factors limiting industrial application this enzyme. In study, we used glucose AtGOD reported with best as source sequence phylogenetic analysis to obtain GOD excellent performance. Six genes were screened...
Cytochrome P450 enzymes (P450s) are promising candidates for the biosynthesis of 25-hydroxyvitamin D3 (25(OH)VD3). However, their industrial application is limited by challenges, such as low stability, inefficient catalysis, and uncoupling reactions. The construction self-sufficient P450s offers a strategic solution to these limitations, but requires linker optimization regulate interdomain conformational dynamics. In this study, we integrated whole-cell biocatalyst screening with systematic...
Objective Bacterial and viral infectious triggers are linked to spondyloarthritis (SpA) including psoriatic arthritis (PsA) development, likely via dendritic cell activation. We investigated spinal entheseal plasmacytoid cells (pDCs) toll-like receptor (TLR)-7 9 activation therapeutic modulation, JAK inhibition. also if COVID-19 infection, a potent TLR-7 stimulator triggered PsA flares. Methods Normal pDCs were characterized stimulated with imiquimod CpG oligodeoxynucleotides (ODN) evaluate...
ELABELA (ELA), a recently discovered peptide, is highly expressed in adult kidneys and the endothelium system. It has been identified as novel endogenous ligand for apelin receptor (APJ). This study aims to investigate role of ELA diabetic glomerular endothelial pyroptosis its underlying mechanism. Initially, significant decrease mRNA levels was observed renal cortex db/db mice high glucose-treated cells (GECs). also found that deficiency ELA+/- significantly accelerated injury, shown by...