A5036965952- Enzyme-mediated dye degradation
- Biofuel production and bioconversion
- Enzyme Production and Characterization
- Amyotrophic Lateral Sclerosis Research
- Microbial Metabolic Engineering and Bioproduction
- Parkinson's Disease Mechanisms and Treatments
- Enzyme Catalysis and Immobilization
- Autophagy in Disease and Therapy
- Endoplasmic Reticulum Stress and Disease
- Microbial Metabolism and Applications
- Neurogenetic and Muscular Disorders Research
- Animal Genetics and Reproduction
- Electrochemical sensors and biosensors
- Cardiac Ischemia and Reperfusion
- Molecular Biology Techniques and Applications
- Advanced Nanomaterials in Catalysis
- Neuroinflammation and Neurodegeneration Mechanisms
- Healthcare and Venom Research
- Polysaccharides and Plant Cell Walls
- Alzheimer's disease research and treatments
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Glycosylation and Glycoproteins Research
- Acupuncture Treatment Research Studies
- Thermodynamic properties of mixtures
- Transgenic Plants and Applications
Chinese Academy of Agricultural Sciences
2014-2025
Institute of Animal Sciences
2021-2025
First Affiliated Hospital of Xi'an Jiaotong University
2012-2024
Xijing Hospital
2013-2024
Air Force Medical University
2007-2024
Xi'an Jiaotong University
2005-2024
Shanghai University of International Business and Economics
2023
Hebei University of Technology
2021-2022
Inner Mongolia University of Technology
2021
Shanghai Ninth People's Hospital
2020
Abstract Ample clinical evidence suggests a high incidence of cardiovascular events in Alzheimer’s disease (AD), although neither precise etiology nor effective treatment is available. This study was designed to evaluate cardiac function AD patients and APP/PS1 mutant mice, along with circulating levels melatonin, mitochondrial aldehyde dehydrogenase (ALDH2) autophagy. mice displayed cognitive myocardial deficits, low ALDH2 activity, autophagy, ultrastructural, geometric (cardiac atrophy...
Abstract Background Heme proteins, such as hemoglobin, horseradish peroxidase and cytochrome P450 (CYP) enzyme, are highly versatile have widespread applications in the fields of food, healthcare, medical biological analysis. As a cofactor, heme availability plays pivotal role proper folding function proteins. However, functional production proteins is usually challenging mainly due to insufficient supply intracellular heme. Results Here, high-heme-producing Escherichia coli chassis was...
Manganese peroxidase is one of the Class II fungal peroxidases that are able to oxidize low redox potential phenolic lignin compounds. For high non-phenolic degradation, mediators such as GSH and unsaturated fatty acids required in reaction. However, it not known whether carboxylic a mediator for degradation. The white rot fungus Irpex lacteus most potent fungi degradation lignocellulose xenobiotics. Two manganese (IlMnP1 IlMnP2) from I. CD2 were over-expressed Escherichia coli successfully...
Manganese peroxidase (MnP) is the one of important ligninolytic enzymes produced by lignin-degrading fungi which has great application value in field environmental biotechnology. Searching for new MnP with stronger tolerance to metal ions and organic solvents maximization potential biodegradation recalcitrant xenobiotics. In this study, it was found that oxalic acid, veratryl alcohol 2,6-Dimehoxyphenol could stimulate synthesis white-rot fungus Irpex lacteus CD2. A novel manganese named as...
Abstract Background The methylotrophic budding yeast Pichia pastoris GS115 is a powerful expression system and hundreds of heterologous proteins have been successfully expressed in this strain. Recently, P. has also exploited as an attractive cell factory for the production high-value biochemicals due to Generally Recognized Safe (GRAS) status high growth rate However, appropriate regulation metabolic flux distribution between product biosynthesis still cumbersome task achieving efficient...
Glucoamylase is an important industrial enzyme in the saccharification of starch into glucose. However, its poor thermostability and low catalytic efficiency limit applications. Therefore, improving these properties glucoamylase great significance for industry. In this study, a novel glucoamylase-encoding gene TlGa15B from thermophilic fungus Talaromyces leycettanus JCM12802 was cloned expressed Pichia pastoris. The optimal temperature pH recombinant were 65 ℃ 4.5, respectively. exhibited...
Aims: Clinical application of cellular therapy for cardiac regeneration is significantly hampered by the low retention engrafted cells, mainly attributable to poor microenvironment dominated inflammation and oxidative stress in host's infarcted myocardium. This study aims at investigating whether liver X receptor (LXR) agonist T0901317 will improve survival adipose-derived mesenchymal stem cells (AD-MSCs) after transplantation into hearts. Results: Noninvasive vivo bioluminescence imaging...
Bone marrow-derived mesenchymal stem cells (BM-MSCs) have been proposed as an ideal autologous cell source for cell-based therapy myocardial infarction (MI). However, decreased viability and impaired function of aged MSCs hampered the therapeutic efficacy engrafted MSCs, underlying mechanisms remain unclarified. Here, we investigated role inositol phosphates 6 kinase (IP6Ks) inhibition on BM-MSCs its mechanism.BM-MSCs isolated from young (8-week-old) or (18-month-old) donor male C57BL/6...
Ligninolytic enzymes, including laccase, manganese peroxidase, and dye-decolorizing peroxidase (DyP), have attracted much attention in the degradation of mycotoxins. Among these possible pathway mycotoxins catalyzed by DyP is not yet clear. Herein, a DyP-encoding gene, StDyP, from Streptomyces thermocarboxydus 41291 was identified, cloned, expressed Escherichia coli BL21/pG-Tf2. The recombinant StDyP capable catalyzing oxidation substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic...
Abstract Serine proteases are among the most important biological additives in various industries such as detergents, leather, animal feed and food. A serine protease gene, Fgapt4 , from Fusarium graminearum 2697 was identified, cloned expressed Pichia pastoris . The optimal pH temperature of Fg APT4 were 8.5 40°C, respectively. relative activity >30% even at 10°C. It had a wide range stability (4.0–12.0) detergent compatibility. To improve catalytic activity, strategy combining molecular...
Cytochrome P450 enzymes (P450s) are promising candidates for the biosynthesis of 25-hydroxyvitamin D3 (25(OH)VD3). However, their industrial application is limited by challenges, such as low stability, inefficient catalysis, and uncoupling reactions. The construction self-sufficient P450s offers a strategic solution to these limitations, but requires linker optimization regulate interdomain conformational dynamics. In this study, we integrated whole-cell biocatalyst screening with systematic...
To investigate the efficacy and feasibility of percutaneous intramyocardial injection bone marrow mesenchymal stem cells (MSC) autologous marrow-derived mononuclear (BMMNC) on cardiac functional improvement in porcine myocardial infarcted hearts.Acute infarction (AMI) was induced 22 minipigs by temporary balloon occlusion left anterior descending coronary artery for 60 min.Two weeks post AMI, BMMNC (n = 7, 245 ± 98 × 10(6)), MSC 8, 56 17 or phosphate buffered saline (PBS; n 7) were injected...
The white rot fungus Irpex lacteus exhibits a great potential in biopretreatment of lignocellulose as well biodegradation xenobiotic compounds by extracellular ligninolytic enzymes. Among these enzymes, the possible involvement dye-decolorizing peroxidase (DyP) lignin degradation is not clear yet. Based on enzyme activities and secretome analysis, I. CD2 produced DyPs main enzymes when grown Kirk's medium supplemented with lignin. Further transcriptome analysis revealed that induced...
N-acetylglucosamine (GlcNAc) is an amino sugar that has been widely used in the nutraceutical and pharmaceutical industries. Recently, microbial production of GlcNAc developed. One major challenge for efficient biosynthesis to achieve appropriate carbon flux distribution between growth production. Here, a synergistic substrate co-utilization strategy was address this challenge. Specifically, glycerol utilized support cell generate glutamine acetyl-CoA, which are acetyl donors, respectively,...
Amyotrophic lateral sclerosis is an unremitting neurodegenerative (ND) disease characterized by progressive and fatal loss of motor neuron function. While underlying mechanisms for ALS susceptibility are complex, current understanding suggests that interactions between age, genetic, environmental factors may be the key. Environmental exposure to metal/metalloids has been implicated in various ND diseases including ALS, Alzheimer's Disease (AD), Parkinson's (PD). However, most currently...