A5100456933- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Cytomegalovirus and herpesvirus research
- Viral-associated cancers and disorders
- Ubiquitin and proteasome pathways
- Herpesvirus Infections and Treatments
- HIV Research and Treatment
- Poxvirus research and outbreaks
- CAR-T cell therapy research
- Virus-based gene therapy research
- Endoplasmic Reticulum Stress and Disease
- Plant Virus Research Studies
- Animal Virus Infections Studies
- Monoclonal and Polyclonal Antibodies Research
- Virology and Viral Diseases
- Peptidase Inhibition and Analysis
- Bacteriophages and microbial interactions
- Cancer Immunotherapy and Biomarkers
- HIV/AIDS drug development and treatment
- Viral gastroenteritis research and epidemiology
- Autophagy in Disease and Therapy
- Hematopoietic Stem Cell Transplantation
- Systemic Lupus Erythematosus Research
- Pediatric Pain Management Techniques
Beijing Shijitan Hospital
2016-2025
Capital Medical University
2016-2025
Henan University of Traditional Chinese Medicine
2025
Yuhuangding Hospital
2024
Shenyang Agricultural University
2023
Changchun Institute of Biological Products
2023
Washington University in St. Louis
2012-2022
Bengbu Medical College
2019-2022
Beijing Center for Disease Prevention and Control
2022
Beijing University of Technology
2008-2021
The mechanism by which substrates for endoplasmic reticulum–associated degradation are retrotranslocated to the cytosol remains largely unknown, although ubiquitination is known play a key role. mouse γ-herpesvirus protein mK3 viral RING-CH–type E3 ligase that specifically targets nascent major histocompatibility complex I heavy chain (HC) degradation, thus blocking immune detection of virus-infected cells. To address question how HC and what role plays in this action, we investigated...
Mucosal-associated invariant T (MAIT) cells have a semi-invariant TCR Vα-chain, and their optimal development is dependent upon commensal flora expression of the nonpolymorphic MHC class I-like molecule MR1. MAIT are activated in an MR1-restricted manner by diverse strains bacteria yeast, suggesting widely shared Ag. Recently, human mouse MR1 were found to bind bacterial riboflavin metabolites (ribityllumazine [RL] Ags) capable activating cells. In this study, we used MR1/RL tetramers study...
Like CD1d-restricted iNKT cells, mucosal-associated invariant T cells (MAITs) are “innate” that express a canonical TCRα chain, have memory phenotype, and rapidly secrete cytokines upon TCR ligation. Unlike MAIT require the class Ib molecule MHC-related protein I (MR1), B gut flora for development and/or expansion, they preferentially reside in lamina propria. Evidence strongly suggests cell activation is ligand-dependent, but nature of MR1 ligand unknown. In this study, we define mechanism...
Ubiquitin (Ub) modification of proteins plays a prominent role in the regulation multiple cell processes, including endoplasmic reticulum–associated degradation (ERAD). Until recently, ubiquitination substrates was thought to occur only via isopeptide bonds, typically lysine residues. Several recent studies suggest that Ub can also be coupled nonlysine residues by ester/thiolester bonds; however, molecular basis for these novel modifications remains elusive. To probe mechanism and importance...
Abstract Despite over 20 years of clinical use, IL-2 has not fulfilled expectations as a safe and effective form tumour immunotherapy. Expression the high affinity IL-2Rα chain on regulatory T cells mitigates anti-tumour immune response its expression vascular endothelium is responsible for life threatening complications such diffuse capillary leak pulmonary oedema. Here we describe development recombinant fusion protein comprised cowpox virus encoded NKG2D binding (OMCP) mutated with poor...
Myct1 inhibition controls tumor angiogenesis, remodels immunity, and improves immunotherapy outcomes in mouse models.
The World Health Organization (WHO) declared monkeypox as a public health emergency of international concern (PHEIC) on July 23, 2022, their highest level alert. This raised concerns about the management global outbreak, well scientific analysis and accurate prediction future course epidemic. study used EpiSIX (an system for epidemics based general SEIR model) to analyze epidemic forecast major tendencies data from USA CDC (https://www.cdc.gov) WHO...
Helminths immunomodulate the host immune system by secreting proteins to create an inhibitory environment as a strategy for survival in host. As bystander effect, this balances reduce hypersensitivity allergens or autoantigens. Based on this, helminth therapy has been used treat some allergic autoimmune diseases. tissue-dwelling helminth, Trichinella spiralis infection identified have strong immunomodulatory effects; effective components worm not yet identified. The soluble extracts of T....
The concurrent presence of COVID-19 infection in advanced cancer patients has increased the mortality since compromised immunity was inevitably worsen. role and clinical impact autologous adoptive T cell immunotherapy (ACT) designed for anti-cancer treatment were not known such circumstances. safety potential immune reconstitution ACT with active have yet unknown as well. effect infused on symptom severity manifestation should be summarized. In this respectively observation study,...
Ubiquitin E3 ligases are important cellular components for endoplasmic reticulum (ER)-associated degradation due to their role in substrate-specific ubiquitination, which is required retrotranslocation (dislocation) of most unwanted proteins from the ER cytosol proteasome degradation. However, our understanding molecular mechanisms how confer recognition, and substrate limited especially mammalian cells. mK3 a type III membrane protein encoded by murine γ herpesvirus 68. As conferred its...
One of the hallmarks viral immune evasion is capacity to disrupt major histocompatibility complex class I (MHCI) antigen presentation evade T-cell detection. Cowpox virus encoded protein CPXV203 blocks MHCI surface expression by exploiting KDEL-receptor recycling pathway, and here we show that directly binds a wide array fully assembled proteins, both classical non-classical. Further, stability CPXV203/MHCI complexes highly pH dependent, with dramatically increased affinities at lower Golgi...
Purpose: Although local oncolytic viral therapy (OVT) may enhance tumor lysis, antigen release, and adaptive immune responses, systemic antitumor responses post-therapy are limited. Adoptive immunotherapy with autologous dendritic cells (DC) cytokine-induced killer (DC–CIK) synergizes therapies. We hypothesized that OVT Herpes Simplex Virus-granulocyte macrophage-colony-stimulating factor (HSV-GM-CSF) would induce T cell could be expanded systemically sequential DC–CIK therapy.Patients...
Current treatments for antibody-mediated autoimmunity are associated with lack of specificity, leading to immunosuppressive effects. To overcome this limitation, we have developed a class antibody-based therapeutics the treatment involving antibodies that recognize autoantigen, myelin oligodendrocyte glycoprotein (MOG). These agents ("Seldegs," selective degradation) selectively eliminate antigen (MOG)-specific without affecting levels other specificities. Seldeg mice during exacerbation...
Sepsis is a life-threateningorgandysfunction caused by the cytokine storm induced severe bacterial infection. Excessive inflammatory responses are responsible for lethal organ damage during early stage of sepsis. Helminth infection and helminth-derived proteins have been identified to ability immunomodulate host immune system reducing inflammation against diseases. Trichinella spiralis cystatin (Ts-Cys) cysteine protease inhibitor with strong immunomodulatory functions on system. Our...
The mK3 protein of gammaherpesvirus 68 and the kK5 Kaposi's sarcoma-associated herpesvirus are members a family structurally related viral immune evasion molecules that all possess RING-CH domain with ubiquitin ligase activity. These proteins modulate expression major histocompatibility complex class I (mK3 kK5) as well other like ICAM-1 B7.2 (kK5). Previously, was shown to ubiquitinate nascent molecules, resulting in their rapid degradation, this process found be dependent on TAP tapasin,...
The human specific poxvirus molluscum contagiosum virus (MCV) produces skin lesions that can persist with minimal inflammation, suggesting the has developed robust immune evasion strategies. However, investigations into underlying mechanisms of MCV pathogenesis have been hindered by lack a model system to propagate virus. Herein we demonstrate MCV-encoded MC80 disrupt MHC-I antigen presentation in and mouse cells. shares moderate sequence-similarity find it associates components...
A plethora of ubiquitin ligases determine the intracellular location and fate numerous proteins in a substrate-specific manner. However, mechanisms for these functions are incompletely understood. Most have structurally related RING domains that critical ligase activity including recruitment conjugating enzymes. Here we probe function RING-CH domain murine gamma-herpesvirus-68 mK3 as an immune evasin by targeting major histocompatibility complex (MHC) class I heavy chains endoplasmic...
Nasopharyngeal carcinoma often occurs in humans the nasopharyngeal epithelium area. Ebstein-Barr (EB) virus plays a key role process of lesions. Early antigen antibody (EA-IgA) and viral capsid IgA (VCA-IgA) EB detection serum can effectively monitor Serum vascular endothelial growth factor (VEGF) -C VEGF-D expression reflect distant metastases ability human tumor cells.153 cases patients our hospital were enrolled, while 148 healthy adults selected as control. ELISA was used to detect...
The ongoing discovery of disease-associated epitopes detected by CD8 T cells greatly facilitates peptide-based vaccine approaches and the construction multimeric soluble recombinant proteins (e.g. tetramers) for isolation enumeration antigen-specific cells. Related to these outcomes epitope is recent demonstration that MHC class I/peptide complexes can be expressed as single chain trimers (SCTs) with peptide, beta(2)m heavy connected linkers form a polypeptide chain. Studies using clinically...