A5101684047- Influenza Virus Research Studies
- HIV Research and Treatment
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Pancreatic and Hepatic Oncology Research
- Respiratory viral infections research
- HIV/AIDS drug development and treatment
- Immune Cell Function and Interaction
- Cancer Genomics and Diagnostics
- Ferroptosis and cancer prognosis
- HIV/AIDS Research and Interventions
- Cytomegalovirus and herpesvirus research
- Animal Virus Infections Studies
- Hepatitis B Virus Studies
- Cancer Research and Treatments
- Viral Infections and Outbreaks Research
- Hepatitis C virus research
- COVID-19 Clinical Research Studies
- Immune Response and Inflammation
- interferon and immune responses
- Viral Infections and Vectors
- RNA modifications and cancer
- HIV-related health complications and treatments
- Immunotherapy and Immune Responses
Zhuhai People's Hospital
2025
Jinan University
2025
Zhejiang University
2016-2025
First Affiliated Hospital Zhejiang University
2013-2025
Beijing Institute of Technology
2025
State Key Laboratory of Diagnosis and Treatment of Infectious Diseases
2015-2024
China Institute of Water Resources and Hydropower Research
2024
Wuhan University of Technology
2024
National Clinical Research Center for Digestive Diseases
2020-2024
Shandong University
2023-2024
Summary The sudden outbreak of the severe acute respiratory syndrome–coronavirus (SARS-CoV-2) has spread globally with more than 1,300,000 patients diagnosed and a death toll 70,000. Current genomic survey data suggest that single nucleotide variants (SNVs) are abundant. However, no mutation been directly linked functional changes in viral pathogenicity. We report characterizations 11 patient-derived isolates. observed diverse mutations these isolates, including 6 different spike...
Abstract The available evidence suggests that alterations in gut microbiota may be tightly linked to the increase microbial translocation and systemic inflammation patients with human immunodeficiency virus 1 (HIV-1) infection. We profiled fecal as a proxy of by parallel barcoded 454-pyrosequencing 67 HIV-1-infected (32 receiving highly active antiretroviral therapy [HAART] 35 HAART naïve) 16 healthy controls from Chinese population. showed α-diversity indices did not differ significantly...
Background Since the first HIV/AIDS case appeared in 1980s, has been focus of international attention. As a major public health problem, there are epidemiological uncertainties about future HIV/AIDS. It is important to monitor global statistics prevalence, deaths, disability adjusted life years (DALYs), and risk factors for adequate prevention control. Methods The Global Burden Disease Study 2019 database was used analyze burden 1990–2019. By extracting global, regional, national data on...
Domestic ducks are natural reservoirs of avian influenza viruses and serve as reassortant hosts for new virus subtypes. We isolated 2 novel A(H5N8) from domestic in eastern China, sequenced their genomes, tested pathogenicity chickens mice. Circulation these may pose health risks humans.
Abstract The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has spread globally with more than 33 million patients diagnosed, taking a lives. Abundant mutations were observed but the functional consequences of these are largely unknown. We report mutation spectrum, replication dynamics, and infectivity 11 patient-derived viral isolates in diverse cell lines, including human lung cancer line Calu-3. 46 mutations, 9 different spike gene. Importantly, show significant consistent...
Abstract Structural principles underlying the composition and synergistic mechanisms of protective monoclonal antibody cocktails are poorly defined. Here, we exploited cooperativity to develop a therapeutic cocktail against SARS-CoV-2. On basis our previously identified humanized cross-neutralizing H014, systematically analyzed fully human naive library rationally potent neutralizing partner, P17, which confers effective protection in animal model. Cryo-EM studies dissected nature P17...
The mutations in the SARS-CoV-2 virus genome during COVID-19 dissemination are unclear. In 788 patients from Zhejiang province, we observed decreased rate of severe/critical cases compared with Wuhan. For mechanisms exploration, isolated one strain (ZJ01) a mild patient. Thirty-five specific gene were identified. Phylogenetic and relative synonymous codon usage analysis suggested that ZJ01 may be potential evolutionary branch SARS-CoV-2. We classified 54 global strains based on base (C or T)...
Among the current five Variants of Concern, infections caused by SARS-CoV-2 B.1.617.2 (Delta) variant are often associated with greatest severity. Despite recent advances on molecular basis elevated pathogenicity using recombinant proteins, architecture intact Delta virions remains veiled. Moreover, pieces evidence for detailed mechanism S-mediated membrane fusion missing. Here, we showed pleomorphic nature from electron beam inactivated samples and reported in situ structure distribution S...
ABSTRACT The highly pathogenic H5N1 avian influenza virus emerged from China in 1996 and has spread across Eurasia Africa, with a continuous stream of new cases human infection appearing since the first large-scale outbreak among migratory birds at Qinghai Lake. role wild birds, which are natural reservoirs for virus, epidemiology raised great public health concern, but their within ecosystem free-ranging terrestrial mammals remains unclear. In this study, we investigated pikas an attempt to...
SUMMARY Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped virus responsible for the COVID-19 pandemic. Despite recent advances in structural elucidation of SARS-CoV-2 proteins and complexes spike (S) with cellular receptor ACE2 or neutralizing antibodies, detailed architecture intact remains to be unveiled. Here we report molecular assembly authentic using cryo-electron tomography (cryo-ET) subtomogram averaging (STA). Native structures S both pre- postfusion...
The two proteases, PLpro and Mpro, of SARS-CoV-2 are essential for replication the virus. Using a structure-based co-pharmacophore screening approach, we developed novel dual-targeted inhibitor that is equally potent in inhibiting Mpro SARS-CoV-2. contains warhead, which can form covalent bond with catalytic cysteine residue either enzyme. maximum rate inactivation comparable to most inhibitors reported viral proteases drugs currently clinical use. inhibition appears be very specific...
Host-virus interactions can significantly impact the viral life cycle and pathogenesis; however, our understanding of specific host factors involved in highly pathogenic avian influenza A virus H7N9 (HPAI H7N9) infection is currently restricted. Herein, we designed synthesized 65 small interfering RNAs targeting genes potentially associated with various aspects RNA cycles. Afterward, HPAI viruses were isolated interference was used to screen for likely be H7N9. Moreover, research entailed...
Abstract Swine influenza viruses (SIVs) cause clinical respiratory symptoms associated with high mortality rates among pigs. Because pigs can be a “mixing vessel” for viruses, the SIV might pose serious threat to animal and human health. In this study, an H3N2 [A/swine/Zhejiang/19/2019(H3N2) (ZJ-SW19)] was isolated from sick pig in Eastern China 2019, its molecular genetics were characterized. Phylogenetic analysis demonstrated hemagglutinin (HA) neuraminidase (NA) segments of ZJ-SW19 are...
Since March 2013, the H7N9 subtype of avian influenza virus (AIV) has become an important zoonotic infectious disease, garnering significant global attention because its potential to affect human health. Establishing a rapid, effective, and sensitive method detect H7 AIVs is crucial for disease control. In this study, we developed graphene oxide multilayer quantum dot-based immunochromatographic strip ultrasensitive detection AIVs. The demonstrated excellent sensitivity, with limit 0.063...