A5100386628- Cancer-related molecular mechanisms research
- MicroRNA in disease regulation
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Circular RNAs in diseases
- Aquaculture disease management and microbiota
- Genomics and Phylogenetic Studies
- Cancer, Hypoxia, and Metabolism
- Physiological and biochemical adaptations
- Extracellular vesicles in disease
- Animal Genetics and Reproduction
- Immune cells in cancer
- Microbial Community Ecology and Physiology
- Legume Nitrogen Fixing Symbiosis
- Animal Behavior and Reproduction
- Genetic and phenotypic traits in livestock
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Plant pathogens and resistance mechanisms
- Soybean genetics and cultivation
- Immune Cell Function and Interaction
- Single-cell and spatial transcriptomics
- Aquaculture Nutrition and Growth
- Virus-based gene therapy research
- RNA Interference and Gene Delivery
- Epigenetics and DNA Methylation
China Pharmaceutical University
2017-2025
Chinese Academy of Fishery Sciences
2020-2025
Qingdao National Laboratory for Marine Science and Technology
2022-2025
Sichuan Agricultural University
2023-2025
Ningbo University
2020-2025
Anhui University of Science and Technology
2024-2025
Anshan Hospital
2024
Renmin Hospital of Wuhan University
2024
Wuhan University
2024
Jiangsu Agri-animal Husbandry Vocational College
2024
Antarctic krill (Euphausia superba) is Earth’s most abundant wild animal, and its enormous biomass vital to the Southern Ocean ecosystem. Here, we report a 48.01-Gb chromosome-level genome, whose large genome size appears have resulted from inter-genic transposable element expansions. Our assembly reveals molecular architecture of circadian clock uncovers expanded gene families associated with molting energy metabolism, providing insights into adaptations cold highly seasonal environment....
Background Apart from triggering host immune responses, macrophages also act as a major reservoir for mycobacteria. For better survival, mycobacteria have evolved various mechanisms to modulate the production of proinflammatory cytokines in macrophages, and manipulation micro-RNA (miRNA) expression has been considered an important one. Methodology/Principal Findings In this study, we found that miR-146a was significantly increased time- dose-dependent manner mycobacteria-infected...
Abstract Base editing tools for cytosine to thymine (C–T) conversion enable genome manipulation at single base-pair resolution with high efficiency. Available base editors (BEs) C–T (CBEs) have restricted scopes and nonnegligible off-target effects, which limit their applications. Here, by screening diversified lamprey cytidine deaminases, we establish various CBEs expanded scopes, could be further refined fusing strategies, either N-terminus or C–terminus of nCas9. Furthermore, analysis...
Abnormal expression of long noncoding RNAs (lncRNAs) has been reported in the acute stage ischemic stroke (AIS). This study aimed to explore differential lncRNA subpopulations peripheral blood mononuclear cells (PBMCs) from AIS patients and further evaluate its underlying mechanisms stroke-induced immunosuppression.We reanalyzed microarray data investigated abnormally expressed lncRNAs PBMCs by magnetic cell sorting real-time quantitative PCR. The potential mechanism small nucleolar RNA host...
Spermidine have been reported a role in antioxidative, antiaging, and antiinflammatory. Oxidative stress causes granulosa cell (GC) apoptosis, follicular atresia, impairs poultry reproductive functions. Studies found that autophagy is the protective mechanism against antioxidant apoptosis cells. However, relationship between spermidine-induced autophagy, oxidative stress, goose GCs remains unclear. In this study, we investigated to mediate spermidine effects on alleviation of GCs. Follicular...
Abstract Chinese sea bass ( Lateolabrax maculatus ) is a highly sought-after commercial seafood species in Asian regions due to its excellent nutritional value. With the rapid advancement of bioinformatics, higher standards for genome analysis compared previously published reference genomes are now necessary. This study presents gapless assembly genome, which has length 632.75 Mb. The sequences were assembled onto 24 chromosomes with coverage over 99% (626.61 Mb), and telomeres detected on...
The DNA damage and repair pathway is considered a promising target for developing strategies against cancer. RAD51, also known as RECA, recombinase that performs the critical step in homologous recombination. RAD51 has recently received considerable attention due to its function tumor progression decisive role resistance chemotherapy. However, pancreatic cancer seldom been investigated. In this report, we provide evidence regulated by KRAS, promotes cell proliferation. Furthermore, aerobic...
AIM:To investigate the biological role of miR-1290 in esophageal squamous cell carcinoma (ESCC) progression and invasion underlying mechanism. METHODS:Quantitative real-time polymerase chain reaction (qRT-PCR) was performed to evaluate expression ESCC tissue samples.The roles proliferation, migration were identified using mimic-transfected cells.In addition, regulatory effect on suppressor cancer (SCAI) evaluated qRT-PCR, Western blot analysis a dual luciferase reporter assay.RESULTS:...
Lampreys are early jawless vertebrates that the key to understanding evolution of vertebrates. However, lack cytomic studies on multiple lamprey organs has hindered progress in this field. Therefore, present study constructed a comprehensive cell atlas comprising 604,460 cells/nuclei and 70 types from 14 tissue samples. Comparison cellular across species revealed most homologous those jawed We discovered acinar- islet-like populations despite parenchymal lampreys, providing evidence...
MicroRNAs (miRNA) have played an important role in carcinogenesis. In this study, Agilent miRNA microarray was used to identify differentially expressed miRNAs esophageal squamous cell carcinoma (ESCC) tissues and miR‐195 downregulated ESCC compared with normal tissues. Moreover, Cdc42 confirmed as target gene of miR‐195. Ectopic expression cells significantly by directly binding its 3′ untranslated regions, induced G1 cycle arrest, leading a significant decrease growth, migration, invasion...
Hypoxia is a risk factor for non-alcoholic fatty liver diseases, leading to permanent imbalance of lipid homeostasis and steatohepatitis. The current study examined the effect HIF-2α, an oxygen-sensitive heterodimeric transcription factor, on hypoxia-induced dysregulation metabolism in HepG2 cells.Studies were conducted C57BL/6 male mice human cells under hypoxic conditions, transfected with HIF-2α-targeted shRNA. mRNA protein expressions key genes relevant determined via RT-qPCR western...
Elucidating cellular architecture and cell-type evolution across species is central to understanding immune system function susceptibility disease. Adaptive immunity a shared trait of the common ancestor cartilaginous bony fishes. However, evolutionary features lymphocytes in these two jawed vertebrates remain unclear. Here, we present single-cell RNA sequencing atlas cells from (white-spotted bamboo shark) (zebrafish Chinese tongue sole) Cross-species comparisons show that same cell types...
Inhibiting the protein neddylation pathway using NEDD8‑activating enzyme inhibitor MLN4924 represents an attractive anticancer strategy having been demonstrated to exhibit promising efficacy and with tolerable levels of toxicity; however, mechanism by which inhibits cell proliferation in human esophageal squamous carcinoma (ESCC) cells requires further investigation. The present study revealed that treatment led G2 cycle arrest enhanced stability Wee1‑like kinase cyclin dependent 1A B p27....