A5023500890- RNA Interference and Gene Delivery
- Nanoplatforms for cancer theranostics
- Advanced biosensing and bioanalysis techniques
- Nanoparticle-Based Drug Delivery
- Immunotherapy and Immune Responses
- CRISPR and Genetic Engineering
- Immune Cell Function and Interaction
- Microbial Community Ecology and Physiology
- T-cell and B-cell Immunology
- Genomics and Phylogenetic Studies
- Cancer Cells and Metastasis
- MicroRNA in disease regulation
- Immune cells in cancer
- Virus-based gene therapy research
- Gut microbiota and health
- Luminescence and Fluorescent Materials
- Cell death mechanisms and regulation
- Extracellular vesicles in disease
- Plant Stress Responses and Tolerance
- CAR-T cell therapy research
- Peanut Plant Research Studies
- Photodynamic Therapy Research Studies
- Crystallization and Solubility Studies
- Porphyrin and Phthalocyanine Chemistry
- Metal complexes synthesis and properties
Anhui Medical University
2020-2025
Ningbo University
2025
Qingdao Agricultural University
2020-2025
ShanghaiTech University
2022-2025
Zunyi Medical University
2010-2024
Zhejiang University
2021-2024
Shanghai University of Medicine and Health Sciences
2020-2024
Army Medical University
2024
Xinqiao Hospital
2024
Ministry of Education of the People's Republic of China
2024
The role of N6-methyladenosine (m6A) modifications in renal diseases is largely unknown. Here, we characterized the N6-adenosine-methyltransferase-like 3 (METTL3), whose expression elevated tubules different acute kidney injury (AKI) models as well human biopsies and cultured tubular epithelial cells (TECs). METTL3 silencing alleviated inflammation programmed cell death TECs response to stimulation by tumor necrosis factor-α (TNF-α), cisplatin, lipopolysaccharide (LPS), whereas...
Abstract Effective reversal of tumor immunosuppression is critical importance in cancer therapy. A multifunctional delivery vector that can effectively deliver CRISPR‐Cas9 plasmid for β‐catenin knockout to reverse constructed. The multi‐functionalized decorated with aptamer‐conjugated hyaluronic acid and peptide‐conjugated combine the cell/nuclear targeting function AS1411 cell penetrating/nuclear translocation TAT‐NLS. Due significantly enhanced enrichment malignant nuclei, genome editing...
Specific cytotoxicity for catalytic nanomedicine triggered by the tumor microenvironment (TME) has attracted increasing interest. In this work, we prepared AgBiS2 hollow nanospheres with narrow bandgaps via rapid precipitation in a weakly polar solvent, which lowered intrinsic energy gap active production of highly reactive hydroxyl radicals (•OH), especially TME. The as-prepared exhibited enhanced optical absorption and high photothermal conversion efficiency (44.2%). addition, structured...
The incidence and severity of acute kidney injury (AKI) is increased yearly in diabetic patients. Although the mechanisms for this remain unclear, prevention AKI nephropathy feasible value. As we detected highly activation TGF-β/Smad3 signaling both human biopsy mouse model nephropathy, hypothesized that Smad3 kidneys may increase sensitivity. We tested our hypothesis vitro using TGF-β type II receptor (TGF-βRII) disrupted tubular epithelial cells (TECs) vivo mice with streptozotocin...
Lipid nanoparticles (LNPs) carrying therapeutic mRNAs hold great promise in treating lung-associated diseases like viral infections, tumors, and genetic disorders. However, because of their thermodynamically unstable nature, traditional LNPs need to be stored at low temperatures, which hinders prevalence. Herein, an efficient lung-specific mRNA delivery platform named five-element (FNPs) is developed helper-polymer poly(β-amino esters) (PBAEs) DOTAP are used combination. The new strategy...
Plant glutamine synthetase (GS, EC6.3.1.2) catalyzes the synthesis of from glutamate and ammonium ions acts as a key enzyme in nitrogen metabolic pathway organisms. Nitrogen is an essential element for plant growth development plays important role crop yield quality formation. Therefore, GS crucial many physiological processes plants. Currently, regulation by plants well-studied terms its effect on development. This article reviews regulatory molecular mechanism mitigating stress injury,...
Heterogeneity of the tumor microenvironment (TME) is primarily responsible for ineffective treatment and uncontrolled progression. Pyroptosis-based immunogenic cell death (ICD) therapy an ideal strategy to overcome TME heterogeneity obtain a satisfactory antitumor effect. However, efficiency current pyroptosis therapeutics, which mainly depends on single endogenous or exogenous stimulus, limited by intrinsic pathological features malignant cells. Thus, it necessary develop synergistic with...
Messenger RNA (mRNA) encoding base editors, along with single guide RNAs (sgRNAs), have emerged as a promising therapeutic approach for various disorders. However, there is still insufficient exploration in achieving targeted and efficient delivery of mRNA sgRNA to multiple organs while ensuring high biocompatibility stability vivo. To address this challenge, we synthesized library 108 poly(β-amino) esters (PBAEs) by incorporating 100% hydrophobic side chains end-caps varying amines. These...
Abstract Targeted delivery of mRNA with lipid nanoparticles (LNPs) holds great potential for treating pulmonary diseases. However, the lack rational design principles efficient lung‐homing lipids hinders prevalence therapeutics in this organ. Herein, combinatorial screening structure‐function analysis is applied to rationalize strategy nonpermanently charged lung‐targeted ionizable lipids. It discovered that carrying N‐methyl and secondary amine groups heads, three tails originated from...
The mode of cell death following photodynamic therapy was investigated from the perspective programmed or apoptosis. Human prostate carcinoma cells (PC3), human non-small lung (H322a) and rat mammary (MTF7) were treated by therapy. An examination extracted cellular DNA gel electrophoresis showed characteristic ladder indicative internucleosomal cleavage during magnitude response dosage required to induce fragmentation different in PC3 MTF7. MTF7 responded with rapid apoptosis at dose light...
Peanut (Arachis hypogaea) consists of two subspecies, hypogaea and fastigiata, has been cultivated worldwide for hundreds years. Here, 158 peanut accessions were selected to dissect the molecular footprint agronomic traits related domestication using specific-locus amplified fragment sequencing (SLAF-seq method). Then, a total 17,338 high-quality single nucleotide polymorphisms (SNPs) in whole genome revealed. Eleven subsequently analyzed genome-wide association studies (GWAS). Candidate...
Drug delivery has become an important strategy for improving the chemotherapy efficiency. Here we developed a multifunctionalized nanosized albumin-based drug-delivery system with tumor-targeting, cell-penetrating, and endolysosomal pH-responsive properties. cRGD-BSA/KALA/DOX nanoparticles were fabricated by self-assembly through electrostatic interaction between cell-penetrating peptide KALA cRGD-BSA, cRGD as tumor-targeting ligand. Under endosomal/lysosomal acidic conditions, changes in...
Recalcitrance to tissue culture and genetic transformation is the major bottleneck for gene manipulation in crops. In barley, immature embryos of Golden Promise have typically been used as explants transformation. However, genotype dependence this approach limits modification commercial varieties. Here, we developed an anther culture-based system that permits effective creation transgenic gene-edited plants from barley The protocol was tested four Australian varieties, which differed...
Abstract Poor selectivity and unintended toxicity to normal organs are major challenges in calcium ion (Ca 2+ ) overload tumor therapy. To address this issue, a cell membrane‐anchoring nano‐photosensitizer (CMA‐nPS) is constructed for inducing tumor‐specific Ca through multistage endogenous homeostasis disruption under light guidance, i.e., the extracellular influx caused by membrane damage, followed intracellular imbalance mitochondrial dysfunction. CMA‐nPS decorated two types of...
The immunogenicity of HLA-A2-restricted T-cell epitopes in the S protein Severe acute respiratory syndrome coronavirus (SARS-CoV) and human strain 229e (HCoV-229e) was analyzed for elicitation a immune response donors who had fully recovered from SARS-CoV infection. We employed online database analysis to compare differences amino acid sequences homologous T HCoV-229e SARS-CoV. identified epitope peptides were synthesized, their binding affinities HLA-A2 validated compared T2 cell system....
Ginsenoside Rg3 is an effective chemical component extracted from the red Panix. The experiment demonstrated that it might effectively inhibit proliferation and metastasis of tumor cells. exact molecular mechanism remains unclear so far. To further explore antitumor function Rg3, we investigated in-vitro in-vivo activity in treatment B16 melanoma cells, derived C57BL/6 mouse, capable forming colonies lungs following intravenous injection. Cell was measured by 3-(4,5)-dimethylthiahiazo...
One of critical steps in genome editing by CRISPR-Cas9 is to deliver the system into targeted cells. In this study, we developed a dual-targeting delivery based on polymer/inorganic hybrid nanoparticles realize highly efficient tumor cells as well situ detection related protein expression edited The plasmid for CDK11 knockout was encapsulated core composed protamine sulfate, calcium carbonate, and phosphate coprecipitation, functional derivatives carboxymethyl chitosan (biotinylated with...
A macrophage and tumor targeting delivery system of pDNA IL-12 can effectively regulate polarity reverse cancer immunoresistance.
To overcome cancer-associated immunosuppression, we prepared a dual-targeting vector to deliver CpG oligodeoxynucleotides (ODN) macrophages. The system composed of mannosylated carboxymethyl chitosan (MCMC)/hyaluronan (HA) for macrophage targeting and protamine sulfate ODN complexation was by self-assembly. effects delivery on immune cells studied in J774A.1 cells. Due the enhanced efficiency, exhibits higher stimulatory activity compared with monotargeting containing either MCMC or HA,...