A5060576707- Inflammasome and immune disorders
- Heme Oxygenase-1 and Carbon Monoxide
- IL-33, ST2, and ILC Pathways
- interferon and immune responses
- Cell death mechanisms and regulation
- Cell Adhesion Molecules Research
- Acute Kidney Injury Research
- Gout, Hyperuricemia, Uric Acid
- Cellular Mechanics and Interactions
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- S100 Proteins and Annexins
- Chronic Kidney Disease and Diabetes
- Cancer-related gene regulation
- Particle accelerators and beam dynamics
- Epigenetics and DNA Methylation
- Nanofabrication and Lithography Techniques
- Cancer Research and Treatments
- Corrosion Behavior and Inhibition
- Aortic aneurysm repair treatments
- Monoclonal and Polyclonal Antibodies Research
- Particle Accelerators and Free-Electron Lasers
- Chemotherapy-induced organ toxicity mitigation
- Neonatal Respiratory Health Research
- Aortic Disease and Treatment Approaches
Wayne State University
2019-2025
Beijing University of Chemical Technology
2024
State Key Laboratory of Food Science and Technology
2024
Jiangnan University
2024
Institute of Modern Physics
2016-2024
Chinese Academy of Sciences
2016-2024
Southern Medical University
2024
Wayne State College
2024
Changchun University of Chinese Medicine
2024
University of Saskatchewan
2024
The NLRP3 inflammasome is a critical component of the innate immune system. activation induced by diverse stimuli associated with bacterial infection or tissue damage, but its inappropriate involved in pathogenesis inherited and acquired inflammatory diseases. However, mechanism which activated remains poorly understood. In this study, we explored role kinases screening kinase inhibitor library identified 3,4-methylenedioxy-β-nitrostyrene (MNS) as an for activation. Notably, MNS did not...
Transplantation of hematopoietic stem cells (HSCs) with a naturally occurring CCR5 mutation confers loss detectable HIV-1 in the patient, making ablation gene HSCs an ideal therapy for cure. Although disruption has been attempted CD4+ T and stem/progenitor (HSPCs), efficient editing high specificity long-term therapeutic potential remains major challenge clinical translation. Here, we established CRISPR/Cas9 system human CD34+ HSPCs achieved evaluated reconstituted NOD/Prkdcscid/IL-2Rγnull...
The role of N6-methyladenosine (m6A) modifications in renal diseases is largely unknown. Here, we characterized the N6-adenosine-methyltransferase-like 3 (METTL3), whose expression elevated tubules different acute kidney injury (AKI) models as well human biopsies and cultured tubular epithelial cells (TECs). METTL3 silencing alleviated inflammation programmed cell death TECs response to stimulation by tumor necrosis factor-α (TNF-α), cisplatin, lipopolysaccharide (LPS), whereas...
ABSTRACT Multiple sclerosis (MS) is a chronic disease that characterized by demyelination and axonal damage in the central nervous system. Cognitive deficits are recognized as one of features MS, these affect patients’ quality life. Increasing evidence from experimental autoimmune encephalomyelitis (EAE), animal model has suggested EAE mice exhibit hippocampal impairment cognitive deficits. However, underlying mechanisms still unclear. The NLRP3 inflammasome key contributor to...
Abstract On the basis of studies in mouse macrophages, activation nucleotide-binding oligomerization domain–like receptor (NLR) pyrin domain–containing 3 (Nlrp3) inflammasome is thought to require two signals. The first signal provided by TLR stimulation and triggers synthesis IL-1β precursor Nlrp3. second can be mediated purinergic P2X ligand–gated ion channel 7 (P2X7) millimolar concentrations ATP. However, these high ATP are not found normally vivo extracellular milieu, raising concern...
Recognition of intracellular pathogenic bacteria by members the nucleotide-binding domain and leucine-rich repeat containing (NLR) family triggers immune responses against bacterial infection. A major response induced several Gram-negative is activation caspase-1 via Nlrc4 inflammasome. Upon activation, regulates processing proIL-1β proIL-18 leading to release mature IL-1β IL-18, induction pyroptosis. The inflammasome requires presence an intact type III or IV secretion system that mediates...
Inflammasomes are multi-protein platforms that drive the activation of caspase-1 leading to processing and secretion biologically active IL-1β IL-18. Different inflammasomes including NOD-like receptor (NLR) family pyrin domain-containing 3 (NLRP3), NLR caspase-recruitment 4 (NLRC4) absent in melanoma 2 (AIM2) activated assembled response distinct microbial or endogenous stimuli. However, mechanisms by which upstream stimuli trigger inflammasome remain poorly understood. Double-stranded...
Chemotaxis allows neutrophils to seek out sites of infection and inflammation. The asymmetric accumulation filamentous actin (F-actin) at the leading edge provides driving force for protrusion is essential development maintenance neutrophil polarity. mechanism that governs cytoskeleton dynamics assembly in has been extensively explored still not fully understood. By using neutrophil-like HL-60 cells, we describe a pivotal role Rictor, component mammalian target rapamycin complex 2 (mTORC2),...
The NLRP3 inflammasome, a critical component of the innate immune system, induces caspase-1 activation and interleukin (IL)-1β maturation in response to microbial infection cellular damage. However, aberrant inflammasome contributes pathogenesis several inflammatory disorders, including cryopyrin-associated periodic syndromes, Alzheimer's disease, type 2 diabetes, atherosclerosis. Here, we identify receptor for activated protein C kinase 1 (RACK1) as complexes macrophages. RACK1 interacts...
Neurodegenerative diseases are a group of neuronal disorders caused by progressive cell death in different regions the human brain. Alzheimer’s disease (AD) and Parkinson’s (PD) most common types neurodegenerative that affect millions people worldwide. There is no cure available for either disease. One pathological hallmarks abnormal protein aggregation central nervous system. Although this major event, innate immunity-mediated neuroinflammation actively contributes to onset progression...
The NLRP3 inflammasome plays a critical role in innate immunity and inflammatory diseases. NIMA-related kinase 7 (NEK7) is essential for activation, its interaction with enhanced by K + efflux. However, the mechanism which efflux promotes this remains unknown. Here, we show that NEK7 rapidly phosphorylated at threonine-190/191 JNK1 downstream of gasdermin D (GSDMD) after activation. phosphorylation enhances binding between NLRP3, further assembly Mutant mice macrophages Thr 190 191 Nek7 were...
The mammalian Hippo kinases, MST1 and MST2, regulate organ development suppress tumor formation by balancing cell proliferation death. In macrophages, inflammasomes detect molecular patterns from invading pathogens or damaged host cells trigger programmed addition to lytic pyroptosis, the signatures associated with apoptosis are induced inflammasome activation, but how coordinate different death processes remains unclear. Here, we identify crucial role of MST1/2 in inflammasome-triggered...
A multiscale simulation model was developed to simulate shape evolution during copper electrodeposition in the presence of additives. The dynamically coupled a kinetic Monte Carlo (KMC) (for surface chemistry and roughness evolution) with finite volume (FV) transport chemical reactions electrolyte) level-set code tracking macroscopic movement metal/electrolyte interface). KMC coarse-grained used multisite mesoparticle approach account for adsorption large molecules. multilevel grid achieve...
Necroptosis plays an essential role in acute kidney injury and is mediated by receptor-interacting protein kinase 1 (RIPK1), 3 (RIPK3), mixed lineage domain-like pseudokinase (MLKL). A novel RIPK3 inhibitor, compound 42 (Cpd-42) alleviates the systemic inflammatory response. The current study was designed to investigate whether Cpd-42 exhibits protective effects on reveal underlying mechanisms.The of were determined vivo through cisplatin- ischaemia/reperfusion (I/R)-induced vitro...
Disruption of endothelial monolayer integrity is the primary instigating factor for many cardiovascular diseases. High density lipoprotein (HDL) oxidized by heme enzyme myeloperoxidase (MPO) dysfunctional in promoting repair. Apolipoprotein A-1 mimetic 4F with its pleiotropic benefits has been proven effective vivo models. In this study we investigated whether promotes repair and restores impaired function HDL (Cl/NO2-HDL) re-endothelialization. We demonstrate that Cl/NO2-HDL act on...
The NLRP3 inflammasome is a critical component of innate immunity that defends the host from microbial infections. However, its aberrant activation contributes to pathogenesis several inflammatory diseases. Activation induces secretion proinflammatory cytokines IL-1β and IL-18 pyroptotic cell death. contains leucine-rich repeat (LRR) domain at C terminus. Although posttranslational modifications in this LRR have been shown regulate activation, role entire remains controversial. Here, we...