A5016832422- MicroRNA in disease regulation
- Circular RNAs in diseases
- Heart Failure Treatment and Management
- Cancer-related molecular mechanisms research
- Fuel Cells and Related Materials
- GDF15 and Related Biomarkers
- RNA Interference and Gene Delivery
- Toxin Mechanisms and Immunotoxins
- Extracellular vesicles in disease
- Cardiac Fibrosis and Remodeling
- Cell Adhesion Molecules Research
- Peptidase Inhibition and Analysis
- Metabolism, Diabetes, and Cancer
- Mechanisms of cancer metastasis
- Ubiquitin and proteasome pathways
- Metabolomics and Mass Spectrometry Studies
- RNA Research and Splicing
- Advanced Biosensing Techniques and Applications
- Heat shock proteins research
- Advanced Proteomics Techniques and Applications
- Cardiovascular Function and Risk Factors
- Protein Tyrosine Phosphatases
- Melanoma and MAPK Pathways
- Cancer, Lipids, and Metabolism
- Cancer, Hypoxia, and Metabolism
National University of Singapore
2007-2025
National University Heart Centre Singapore
2016-2025
National University Health System
2015-2023
Singapore Clinical Research Institute
2015
National University Cancer Institute, Singapore
2005
The receptor tyrosine kinase ErbB2 (HER-2/neu) is overexpressed in up to 30% of breast cancers and associated with poor prognosis an increased likelihood metastasis especially node-positive tumors. In this proteomic study, identify the proteins that are aggressive phenotype HER-2/neu-positive cancer, tumor cells from both -negative tumors were procured by laser capture microdissection. Differentially expressed two subsets identified two-dimensional electrophoresis MALDI-TOF/TOF MS/MS. We...
Abstract INTRODUCTION The prognostic utility of plasma proteomics for cognitive decline and dementia in a Southeast Asian population characterized by high cerebrovascular disease (CeVD) burden is underexplored. METHODS We examined this Singaporean memory clinic cohort 528 subjects ( n = 300, CeVD; 167, incident decline) followed‐up 4 years. RESULTS Of 1441 proteins surveyed, 12‐protein signature significantly predicted q ‐value < .05). Sixteen diverse biological processes were implicated...
The human epidermal growth factor receptor, type 2 (HER-2/neu or c-erbB-2) is a 185 kDa transmembrane protein that phosphorylated upon ligand binding and dimerization with members of the HER/c-erbB family regulates cell differentiation. Its overexpression strongly associated advanced disease, metastasis poor clinical outcome. To better understand mechanisms underlying prognosis breast tumors HER-2/neu-positive status, parallel proteomic analyses were performed on estrogen receptor-negative...
Abnormal amplification/expression of HER-2/neu oncogene has been causally linked with tumorigenesis and metastasis in breast cancer associated shortened overall survival patients. Recently, heat shock protein 27 (Hsp27) was reported to be highly expressed positive tumors cell lines. However, putative functional links between phosphorylation Hsp27 status other clinicopathological features remain elucidated.Comparative phosphoproteomic studies -negative revealed that Hsp27, one the identified...
Study of microRNA (miRNAs) using sheep models is limited due to lack miRNA information. We therefore investigated oar-miRNAs and their regulation in an ovine model heart failure (HF). Left ventricular (LV) tissue was collected from normal (Cont), HF (LV pacing @ ~220bpm for 13-days) HF-recovery (HF-R, 26-days after cessation). MiRNA expression profiled next-generation sequencing (NGS) array, validated by stem-loop qPCR. Detected sequences were mapped against the genome (Oar v4.0) aligned...
We tested the hypothesis that Let-7d-3p contributes to cardiac cell protection during hypoxic challenge. Myoblast H9c2 cells and primary neonatal rat ventricular cardiomyocytes (NRVM) were transfected with five selected miRNA mimics. Both lines subjected 0.2% oxygen hypoxia. The protective effects of these miRNAs determined by assessment metabolic activity CCK8 assay measurement lactate dehydrogenase (LDH) release as a marker injury. Apoptosis autophagy flux assessed Annexin V/7-AAD double...
Natriuretic Peptide Receptor 3 (NPR3), the clearance receptor for extracellular bio-active natriuretic peptides (NPs), plays important roles in homeostasis of body fluid volume and vascular tone. Using luciferase reporter antagomir-based silencing assays, we demonstrated that expression NPR3 could be modulated by microRNA-143 (miR-143-3p), a microRNA species with up-regulated circulating concentrations clinical heart failure. The regulatory effect miR-143 on was further evidenced reciprocal...
Abstract The human epidermal growth factor receptor type 2 (HER‐2/ neu ) oncoprotein is overexpressed in about 30% of breast cancers and associates with metastatic phenotypes tumours. Dissecting the HER‐2/ ‐modulated molecules cancer will be helpful elucidating underlying molecular mechanisms ‐driven tumourigenesis. We investigated differential proteome profiles between microdissected ‐positive ‐negative tumours unambiguously identified 21 proteins diverse biological functions by peptide...
Introduction: Circulating microRNA (miRNAs) profiles are dysregulated in heart failure (HF) suggesting possible diagnostic and prognostic applications. In addition, miRNAs may yield insights into the pathophysiology of HF provide targets for therapeutic intervention. We sought to identify utility further investigate selected as potential Hypothesis: hypothesized that distinct circulating be potentially important therapy improve outcomes HF. Methods: Plasma levels were profiled from 338...
Circulating microRNA (miRNAs) levels are dysregulated in heart failure (HF) suggesting possible diagnostic and prognostic applications. In addition, miRNAs may yield insights into the pathophysiology of HF provide targets for therapeutic intervention. We sought to identify utility further investigate selected as potential hypothesized that distinct circulating be improve outcomes HF. Methods: Plasma from 338 well-characterized patients 208 age-matched controls were collected qPCR based miRNA...
Introduction: The sheep ( Ovis aries ) provides a large animal model in cardiovascular research including heart failure (HF). However, microRNA (miR) related work the has been limited due to paucity of information regarding oar-miR. aim this study was identify novel oar-miRs myocardium and examine their regulation HF recovery. Methods: Heart tissue harvested from undergoing 1. induced by rapid left ventricular (LV) pacing at 225bpm for 14 days, 2. recovery (HF-R) after discontinuation an...
Cardiac natriuretic peptides (NPs) play important roles in the regulation of intravascular blood volume and vascular tone. Among other clearance mechanisms, bio-active circulating NPs are removed by receptor, Natriuretic Peptide Receptor 3 (NPR3). We hypothesized that level NPR3 could be modulated microRNAs (miRNAs) resulting changes bioactivity NPs. have previously reported a cluster miRNAs potentially regulating expression. To extend these findings, expression concerned was examined...