A5040003637- Infectious Diseases and Tuberculosis
- Tuberculosis Research and Epidemiology
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
- Biosensors and Analytical Detection
- Bacterial Infections and Vaccines
- Cancer Immunotherapy and Biomarkers
- Ovarian cancer diagnosis and treatment
- Mycobacterium research and diagnosis
- Gastric Cancer Management and Outcomes
- Cancer-related molecular mechanisms research
- Cancer Cells and Metastasis
- Radiomics and Machine Learning in Medical Imaging
- Gastrointestinal Tumor Research and Treatment
- IgG4-Related and Inflammatory Diseases
- RNA and protein synthesis mechanisms
- Advanced Nanomaterials in Catalysis
- Diagnosis and treatment of tuberculosis
- Ubiquitin and proteasome pathways
- Molecular Communication and Nanonetworks
- Inflammatory Biomarkers in Disease Prognosis
- Cancer-related Molecular Pathways
- PI3K/AKT/mTOR signaling in cancer
- Cancer Mechanisms and Therapy
- MicroRNA in disease regulation
National University of Singapore
2013-2023
National University Hospital
2022
National University Cancer Institute, Singapore
2013-2022
Singapore College of Traditional Chinese Medicine
2018
Institute of Molecular and Cell Biology
2014
Agency for Science, Technology and Research
2014
Immune checkpoint inhibitors (ICI) are now standard-of-care treatment for patients with metastatic gastric cancer (GC). To guide patient selection ICI therapy, programmed death ligand-1 (PD-L1) biomarker expression is routinely assessed via immunohistochemistry (IHC). However, an increasing number of approved ICIs, each paired a different PD-L1 antibody IHC assay used in their respective landmark trials, there unmet clinical and logistical need harmonization. We investigated the...
BACKGROUND. Matrix metalloproteinases (MMPs) are key regulators of tissue destruction in tuberculosis (TB) and may be targets for host-directed therapy. We conducted a phase II double-blind, randomized, controlled trial investigating doxycycline, licensed broad-spectrum MMP inhibitor, patients with pulmonary TB.
Despite the importance of nucleic acid testing in managing COVID-19 pandemic, current detection approaches remain limited due to their high complexity and extensive processing. Here, we describe a molecular nanotechnology that enables direct sensitive viral RNA targets native clinical samples. The technology, termed catalytic amplification by transition-state switch (CATCH), leverages DNA-enzyme hybrid complexes form switch. By ratiometric tuning its constituents, multicomponent is prepared...
WW domain binding protein 2 (WBP2), a transcriptional coactivator, plays vital role in breast tumorigenesis. It positively regulates estrogen receptor, Hippo, and Wnt pathways, which subsequently enhance the transcription of downstream target genes contributing to cancer. Understanding regulation expression activity WBP2 oncoprotein has implication cancer therapy. We have previously reported that is regulated at post-translational post-transcriptional levels. However, its level not known. In...
Abstract Cambodia has one of the highest tuberculosis (TB) incidence rates in WHO Western Pacific region. Remarkably though, prevalence multidrug-resistant TB (MDR-TB) remains low. We explored genetic diversity Mycobacterium (MTB) circulating this unique setting using whole-genome sequencing (WGS). From October 2017 until January 2018, we collected hundred sputum specimens from consenting adults older than 21 years age, newly diagnosed with bacteriologically confirmed 3 districts Phnom Penh...
Understanding the pathophysiology of central nervous system tuberculosis (CNS-TB) is hampered by lack a good pre-clinical model that mirrors human CNS-TB infection. We developed murine demonstrates neurobehavioral changes with similar immunopathology CNS-TB.
Accessible and adaptable nucleic acid diagnostics remains a critical challenge in managing the evolving COVID-19 pandemic. Here, an integrated molecular nanotechnology that enables direct programmable detection of SARS-CoV-2 RNA targets native patient specimens is reported. Termed synergistic coupling responsive equilibrium enzymatic network (SCREEN), technology leverages tunable, catalytic nanostructures to establish interconnected, collaborative architecture. SCREEN mimics extraordinary...
4026 Background: Immune checkpoint inhibitors (ICI) are now standard-of-care treatment for patients with metastatic gastric cancer (GC). To guide patient selection ICI therapy, programmed death ligand-1 (PD-L1) biomarker expression is routinely assessed via immunohistochemistry (IHC). Regulatory approval ICIs granted based on PD-L1 status, scored using metrics such as the combined positive score (CPS). However, an increasing number of approved ICIs, each paired a different antibody IHC assay...
e21095 Background: It is well known that chronic inflammation can enable immune escape by suppressing surveillance. This promotes cancer growth and development, which forms the basis of checkpoint inhibitors use. Pan-Immune-Inflammation Value (PIV), a type immune-inflammatory biomarker, has shown predictive value in various patient populations (colorectal, melanoma, breast, oesophagus, ALK-positive lung cancer) patients with ANCA-associated vasculitis. We aimed to evaluate role PIV as...
Programmable Detection of SarS-coV-2In article number 2101155, Huilin Shao and co-workers report the collaborative coupling enzyme-DNA nanostructures to establish multi-enzyme catalysis.Through programmable favorable equilibrium architecture, technology achieves direct sensitive detection SARS-CoV-2 RNA in native patient samples, within 30 minutes at room temperature.
Abstract Purpose: Metastases account for more than 90% of cancer deaths and hence, inhibiting metastasis is an attractive therapeutic option. Accumulating evidences show that invasion ovarian carcinoma could be driven by Epithelial-Mesenchymal Transition (EMT), a developmental program being employed during progression. As EMT reversible process, or reversing thus emerges as potential anti-metastasis strategy. spectrum consisting the binary epithelial mesenchymal states well transitional...
Intro: Tuberculosis (TB) continues to be a major public health problem around the world.Due lack of effective vaccines and emergence drug resistance in bacteria, development leads with novel mechanisms few side effects is critical.Pantothenate kinase (PANK) involved synthesis Coenzyme A (CoA), an important cofactor component Mycobacterium tuberculosis metabolism.These findings suggest that Pank would target for antimicrobials action.Methods: In this endeavour, ligand-based pharmacophore...
Abstract Despite anti-tuberculous treatment (ATT), central nervous system tuberculosis (CNS-TB) still cause permanent neurological deficits and death. To identify prognostic factors, we profiled a prospective cohort of tuberculous meningitis (TBM) non-TBM patients. We determined significantly increased cerebrospinal fluid (CSF) matrix metalloproteinases (MMPs) neutrophil extracellular traps (NETs) are up-regulated in TBM patients with neuroradiological abnormalities poor outcomes. dissect...
ABSTRACT Background Understanding the pathophysiology of central nervous system tuberculosis (CNS-TB) is hampered by lack a good pre-clinical model that mirrors human CNS-TB infection. We developed murine demonstrates neurobehavioral changes with similar immunopathology CNS-TB. Methods injected two Mycobacterium ( M . tb ) strains, H37Rv and CDC1551, respectively, into mouse C3HeB/FeJ Nos2 -/- mice, either third ventricle or intravenous. compared neurological symptoms, histopathological...
Abstract BackgroundUnderstanding the pathophysiology of central nervous system tuberculosis (CNS-TB) is hampered by lack a good pre-clinical model that mirrors human CNS-TB infection. We developed murine demonstrates neurobehavioral changes with similar immunopathology CNS-TB. MethodsWe injected two Mycobacterium ( M.tb ) strains, H37Rv and CDC1551, respectively, into mouse C3HeB/FeJ Nos2 -/- mice, either third ventricle or intravenous. compared neurological symptoms, histopathological...