A5085469427- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- Transplantation: Methods and Outcomes
- Acute Myeloid Leukemia Research
- Immune Response and Inflammation
- Cytomegalovirus and herpesvirus research
- Polyomavirus and related diseases
- Antifungal resistance and susceptibility
- Hematological disorders and diagnostics
- T-cell and B-cell Immunology
- Mesenchymal stem cell research
- Sphingolipid Metabolism and Signaling
- T-cell and Retrovirus Studies
- Renal Transplantation Outcomes and Treatments
- Chemokine receptors and signaling
- Animal Disease Management and Epidemiology
- Inflammation biomarkers and pathways
- Vector-Borne Animal Diseases
- Immunotherapy and Immune Responses
- Fungal Infections and Studies
- Mycobacterium research and diagnosis
- Neutropenia and Cancer Infections
- Mast cells and histamine
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Asthma and respiratory diseases
The University of Tokyo
2013-2024
University of Tokyo Hospital
2010-2024
Juntendo University
2017-2019
Advanced Medical Research Institute
2019
Hudson Institute
2019
John Wiley & Sons (United States)
2019
Tokyo Medical University Hospital
2018
Tokyo University of Science
2017
Tokyo Medical and Dental University
2010
Recurrent mutations in the gene encoding additional sex combs-like 1 (ASXL1) are found various hematologic malignancies and associated with poor prognosis. In particular, ASXL1 common patients myelodysplasia, including myelodysplastic syndromes (MDSs), chronic myelomonocytic leukemia. Although loss-of-function promote myeloid transformation, a large subset of is thought to result stable truncation ASXL1. Here we demonstrate that C-terminal–truncating Asxl1 (ASXL1-MTs) inhibited...
Low cryopreserved total nucleated cell (TNC) dose in a cord blood (CB) unit has been shown to be associated with engraftment failure and mortality after single-unit transplantation (CBT) adults. Although CB banks offer specific characteristics of dose, such as TNC, CD34+ cells, colony-forming for granulocyte/macrophage (CFU-GM), the impact each on outcomes CBT adults remains unclear. We retrospectively analyzed results 306 CBTs 261 adult patients our institution between 1998 2016. The median...
<h3>Objective</h3> Extracellular ATP mediates mast cell-dependent intestinal inflammation via P2X7 purinoceptors. We have previously shown that CD300f (also called the leucocyte mono-immunoglobulin-like receptor 3 (LMIR3)) suppresses immunoglobulin E-dependent and allergic responses by binding to ceramide. The aim of present study was clarify role ceramide–LMIR3 interaction in development IBD. <h3>Design</h3> dextran sodium sulfate (DSS)-induced colitis model used wild-type (WT),...
Mucosal-associated invariant T (MAIT) cells are a type of innate lymphocyte and recognize riboflavin (vitamin B2) synthesis products presented by MHC-related protein 1. We investigated long-term reconstitution MAIT its association with chronic graft-versus-host disease (cGVHD) in cross-sectional cohort 173 adult patients after allogeneic hematopoietic cell transplantation. According to donor source, the number significantly correlated time cord blood transplantation (CBT) but not bone marrow...
Highlights•We examined the relationship between serum cytokine profiles and pre-engraftment syndrome in 44 adult patients who received cord blood transplantation•Serum levels of 21 cytokines were measured by multiplex bead assays using a flow cytometer.•The elevations IL-5 IL-6 at 2 weeks associated with syndrome•The conversion from naïve to memory phenotype T cells 4 8 was observed syndromeAbstractClinical manifestation high-grade fever skin rash before neutrophil engraftment, termed (PES)...
LPS triggers inflammatory responses; however, the negative regulation of responses in vivo remains poorly understood. CD300f is an inhibitory receptor among CD300 family paired activating and receptors. We have previously identified ceramide as a ligand for shown that binding to inhibits IgE-mediated mast cell activation allergic mouse models. Here we identify critical role inhibiting LPS-induced skin inflammation. deficiency remarkably enhanced edema neutrophil recruitment mice. Higher...
The morphological discrimination of leukemic from non-leukemic T cells is often difficult in adult T-cell leukemia (ATL) as ATL show diversity, with the exception typical "flower cells." Because defects expression CD3 well CD7 are common cells, we applied multi-color flow cytometry to detect a putative leukemia-specific cell population peripheral blood patients. CD4(+) CD14(-) subjected two-color analysis based on vs plot clearly demonstrated presence CD3(dim) CD7(low) subpopulation each...
Transforming growth factor‐β (TGF‐β)‐stimulated clone‐22 (TSC‐22), also called TSC22D1‐2, is a putative tumor suppressor. We previously identified TSC‐22 downstream of an active mutant fms‐like tyrosine kinase‐3 (Flt3). Here, we show that works as suppressor through inhibiting Ras/Raf signaling. Notably, was upregulated by activation, whereas its upregulation inhibited concurrent STAT5 activation. Although normally retained in the cytoplasm nuclear export signal (NES), activation caused...
CD300C is highly homologous with an inhibitory receptor CD300A in immunoglobulin-like domain among the human CD300 family of paired immune receptors. To clarify precise expression and function CD300C, we generated antibodies discriminating between which recognized a unique epitope involving amino acid residues CD300A(F56-L57) CD300C(L63-R64). Notably, was expressed monocytes mast cells. Cross-linking by its specific antibody caused cytokine/chemokine production Fc γ indispensable for both...
Purpose In a recent study to purify adult T-cell leukemia-lymphoma (ATL) cells from acute-type patients by flow cytometry, three subpopulations were observed in CD3 versus CD7 plot (H: CD3highCD7high; D: CD3dimCD7dim; L: CD3dimCD7low). The majority of leukemia enriched the L subpopulation and same clone was included D subpopulations, suggesting clonal evolution. this study, we analyzed with indolent-type ATL human virus type I (HTLV-I) asymptomatic carriers (ACs) see whether profile...
Leukocyte mono-Ig-like receptor 5 (LMIR5, also called CD300b) is an activating expressed in myeloid cells. We have previously demonstrated that T cell Ig mucin 1 works as a ligand for LMIR5 mouse ischemia/reperfusion injury of the kidneys. In this article, we show implicated LPS-induced sepsis mice. Notably, neutrophils constitutively released soluble form (sLMIR5) through proteolytic cleavage surface LMIR5. Stimulation with TLR agonists augmented release sLMIR5. LPS administration or...
Abstract Sepsis is a serious clinical problem. Negative regulation of innate immunity associated with sepsis progression, but the underlying mechanisms remains unclear. Here we show that receptor CD300f promotes disease progression in sepsis. −/− mice were protected from death after cecal ligation and puncture (CLP), murine model septic peritonitis. was highly expressed mast cells recruited neutrophils peritoneal cavity. Analysis (e.g., cell-deficient mice) receiving transplants wild-type or...
Highlights•We designed a reduced-toxicity myeloablative conditioning regimen consisting of fludarabine 180 mg/m2, busulfan 9.6 mg/kg, 4 Gy TBI, and G-CSF–combined Ara-C for elderly patients with nonremission myeloid malignancies in single cord blood transplantation.•The cumulative incidence neutrophil engraftment was 91% at day 42 after CBT.•The probabilities overall survival, disease-free relapse 2 years were 62%, 52%, 26%, respectively.AbstractThe optimal intensity might be dependent on...
CD300 molecules (CD300s) belong to paired activating and inhibitory receptor families, which mediate immune responses. Human CD300e (hCD300e) is expressed in monocytes myeloid dendritic cells transmits an immune-activating signal by interacting with DNAX-activating protein 12 (DAP12). However, the ortholog mice (mCD300e) poorly characterized. Here, we found that mCD300e also receptor. We engagement triggers cytokine production mCD300e-transduced bone marrow–derived mast (BMMCs). Loss of...
Platelet transfusion refractoriness (PTR) is frequently observed after allogeneic hematopoietic cell transplantation (HCT). However, the incidence of and risk factors for PTR, impact PTR on transplant outcomes cord blood (CBT) have not been fully investigated. We retrospectively analyzed 185 adult patients who received single-unit CBT in our institute. The mean 16-hour corrected count increment (CCI) 5840 platelet transfusions was 3.68 × 109/L. Among them, 3196 (54.7%) were associated with a...
The different effects of pre-engraftment syndrome (PES) and acute graft-versus-host disease (aGVHD) on outcomes after cord blood transplantation (CBT) are unclear. We retrospectively evaluated the impact PES aGVHD relapse survival single-unit CBT in 138 adult patients with hematologic malignancies at our institution between 2004 2016. Multivariate analysis demonstrated that development grade III-IV aGVHD, particularly gut or liver involvement, significantly contributed to higher nonrelapse...
Markers of inflammatory and nutritional status, such as the Controlling Nutritional Status (CONUT) score, Prognostic Index, Glasgow Score, C-reactive protein-albumin ratio (CAR) has been demonstrated to be associated with poor prognosis in patients various cancers. Although relatively low cell dose a single cord blood unit restricts indication for transplantation (CBT) pediatric smaller lighter adult patients, impact malnutrition on outcomes after CBT is unclear. We retrospectively analyzed...
A yeast cell wall component stimulates dendritic cells to recruit neutrophils and initiate inflammation in mice.