A5084454121- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Bacterial Identification and Susceptibility Testing
- Antibiotics Pharmacokinetics and Efficacy
- Antibiotic Resistance in Bacteria
- Nail Diseases and Treatments
- Urinary Tract Infections Management
- Clostridium difficile and Clostridium perfringens research
- Antimicrobial Resistance in Staphylococcus
- Fungal and yeast genetics research
- Reproductive tract infections research
- Immune Cell Function and Interaction
- Infectious Diseases and Mycology
- Antibiotic Use and Resistance
- Peptidase Inhibition and Analysis
- Probiotics and Fermented Foods
- Antimicrobial Peptides and Activities
- Polysaccharides and Plant Cell Walls
- Plant-Microbe Interactions and Immunity
- Diphtheria, Corynebacterium, and Tetanus
- Streptococcal Infections and Treatments
- Immune Response and Inflammation
- Viral gastroenteritis research and epidemiology
- Mycobacterium research and diagnosis
- Autophagy in Disease and Therapy
National Institute of Infectious Diseases
2016-2025
National Institutes of Health
2011-2023
National Institute of Allergy and Infectious Diseases
2023
Fukuoka University
2021
Chiba University
2007-2014
Chiba University Hospital
2007-2011
Nihon University
2005
Gifu University of Medical Science
1996-1999
Khon Kaen University
1996-1997
Gifu University
1982-1995
Toxigenic strains of Clostridium difficile are causative agents pseudomembranous colitis and antimicrobial agent-associated diarrhea colitis. The toxigenicity is routinely assayed by using highly sensitive cell cultures. We used a simple rapid polymerase chain reaction (PCR) assay to differentiate toxigenic nontoxigenic C. difficile. Two sets oligonucleotide primer pairs derived from nonrepeating sequences the toxin A gene were amplify 546- 252-bp DNA fragments. pair repeating was 1,266-bp...
The intestinal resident Candida glabrata opportunistically infects humans. However few genetic factors for adaptation in the intestine are identified this fungus. Here we describe C. CYB2 gene encoding lactate dehydrogenase as an factor survival intestine. was a virulence by silkworm infection study. To determine function of CYB2, analysed vitro phenotypes mutant Δcyb2. Δcyb2 grew well glucose medium under aerobic and anaerobic conditions, not supersensitive to nitric oxide which has...
In the pathogenic yeast Candida glabrata, gene targeting to generate knockouts and "knockins" is a potentially powerful method for analysis of function. Its importance increased after C. glabrata genome sequence project, but progress in field hampered by inefficient mechanisms targeting. With use 40-bp homologous flanking DNA, no was identified. To address this issue, YKU80 disrupted, leading an increase efficiency 5.1% using DNA. harness beneficial effects inactivation on frequency without...
Zn[2]-Cys[6] binuclear transcription factors Upc2p and Ecm22p regulate the expression of genes involved in ergosterol biosynthesis exogenous sterol uptake Saccharomyces cerevisiae. We identified two UPC2/ECM22 homologues pathogenic fungus Candida glabrata which we designated CgUPC2A CgUPC2B. The contribution these to homeostasis was investigated. Cells that lack (upc2AΔ) exhibited enhanced susceptibility inhibitors, fluconazole lovastatin, whereas upc2BΔ-mutant cells were as susceptible...
Pulsed-field gel electrophoresis (PFGE) analysis of restriction pattern polymorphism was applied to type Clostridium difficile isolated from neonates hospitalized in a neonatal intensive care unit, and the results were compared with those immunoblot analysis. C. fecal specimens 41 (61%) 67 during 5-month investigation. All these asymptomatic. Fifty-five isolates 32 patients analyzed by PFGE after digestion SmaI SacII endonucleases immunoblotting 10 different antisera. Fifty-three 55 30...
Candida glabrata, a haploid budding yeast, is the cause of severe systemic infections in immune-compromised hosts. The amount free iron supplied to C. glabrata cells during severely limited by iron-chelating proteins such as transferrin. Thus, iron-deficiency response thought play important roles their survival inside host's body. In this study, we found that mitophagy was induced under iron-depleted conditions, and disruption gene homologous ATG32, which responsible for Saccharomyces...
Cryptococcus neoformans causes life-threatening diseases mainly in immunosuppressed hosts such as AIDS patients; C. gattii disseminated infections even healthy hosts. To identify the possible molecular mechanisms underlying this difference virulence, we investigated survival and histopathology of lung tissue wild-type CD4-depleted mice infected with H99 JP02 (the highly virulent strain isolated Japan); then compared dendritic cell (DC) cytokine release responses to different fractions from...
Cryptococcosis due to a highly virulent fungus, Cryptococcus gattii, emerged as an infectious disease on Vancouver Island in Canada and surrounding areas 1999, causing deaths among immunocompetent individuals. Previous studies indicated that C. gattii strain R265 isolated from the Canadian outbreak had immune avoidance or suppression capabilities. However, protective immunity against has not been identified. In this study, we used gain-of-function approach investigate infection using...
The susceptibilities of 462 clinical anaerobic bacterial isolates to N-formimidoyl thienamycin and 16 other currently available investigational antibiotics were determined by the agar dilution technique. N-Formimidoyl was significantly more active than reference against most organisms tested, especially Bacteroides sp., including clindamycin-resistant strains. All inhibited 4 micrograms per ml, no resistant strains found in species tested. less (i.e., had a higher 50% minimal inhibitory...
Summary During disseminated infection by the opportunistic pathogen C andida glabrata , uptake of sterols such as serum cholesterol may play a significant role during pathogenesis. The ATP ‐binding cassette transporter A us1p is thought to function sterol importer and in this study, we show that exogenous occurred under anaerobic conditions wild‐type cells . but not AUS 1 ‐deleted mutant ( aus1 Δ) cells. In aerobic cultures, growth inhibition fluconazole was prevented presence serum,...
Limited data are available on breakthrough fungemia, defined as fungemia that develops administration of antifungal agents, in patients with hematological disorders. We reviewed the medical and microbiological records adult diseases who had between January 2008 July 2019 at Toranomon Hospital Kajigaya Japan. A total 121 cases were identified. Of involved patients, 83, 11, 5, 22 receiving micafungin, voriconazole, itraconazole, liposomal amphotericin B, respectively, when occurred. causative...
The beta-lactamase from Bacteroides fragilis GAI-30144 hydrolyzed imipenem, oxyiminocephalosporins, cephamycins, and penicillins. Enzyme activity was inhibited by EDTA. Zinc completely reversed inactivation of the enzyme molecular mass purified estimated to be 33,000 daltons.
Abstract Sepsis is a serious clinical problem. Negative regulation of innate immunity associated with sepsis progression, but the underlying mechanisms remains unclear. Here we show that receptor CD300f promotes disease progression in sepsis. −/− mice were protected from death after cecal ligation and puncture (CLP), murine model septic peritonitis. was highly expressed mast cells recruited neutrophils peritoneal cavity. Analysis (e.g., cell-deficient mice) receiving transplants wild-type or...
The vaginal flora of 118 Japanese and 208 Thai pregnant women were investigated for the presence bacterial vaginosis (BV), BV-associated organisms, enzyme. A similar prevalence ofBV was found among (13.6%) (15.9%). microbial with BV complex; mean number isolates recovered in group ∼2 times more than that a healthy women. Prevotella species, Porphyromonas Peptostreptococcus Mobiluncus Gardnerella vaginalis, H2O2-nonproducing lactobacilli significantly associated BV. These organisms less...
We investigated the cellular function of Msi3p, belonging to heat shock protein 70 family, in Candida albicans. The mutant strain tetMSI3 was generated, which MSI3 controlled by a tetracycline-repressive promoter, because there is evidence suggest that an essential gene. expression level doxycycline (DOX) and compared its phenotype with control promoter wild-type copy MSI3. results indicated for cell growth. In addition, all tetMSI3-infected mice survived after DOX administration. Drug...
Mannans are mannose polymers attached to cell wall proteins in all Candida species, including the pathogenic fungus albicans. sensed by pattern recognition receptors expressed on innate immune cells. However, detailed structural patterns affecting sensing not fully understood because mannans have a complex structure that includes α- and β-mannosyl linkages. In this study, we focused β-1,2-mannosides of N-linked mannan C. albicans moiety is present non-pathogenic yeast Saccharomyces...
Objectives:Candida species are a major cause of hospital infections, including ocular candidiasis, but few studies have examined the propensities specific to invade eye or unique immunological responses induced. This study frequency and characteristics species-specific Candida infections by epidemiology experiments using mouse candidiasis model. Methods: We reviewed medical records candidemia patients from January 2012 March 2017. also evaluated fungal burden, inflammatory cytokine chemokine...
In response to stimulants, such as serum, the yeast cells of opportunistic fungal pathogen Candida albicans form germ tubes, which develop into hyphae. Yvh1p, one 29 protein phosphatases encoded in C. genome, has 45 % identity with dual-specific phosphatase Yvh1p model Saccharomyces cerevisiae . this study, expression was not observed during initial step tube formation, although expressed constitutively cell cycle progression or hyphal cells. an attempt analyse function phosphatase, complete...