A5062110934- Acute Myeloid Leukemia Research
- Epigenetics and DNA Methylation
- Amyloidosis: Diagnosis, Treatment, Outcomes
- RNA modifications and cancer
- RNA Research and Splicing
- Protein Degradation and Inhibitors
- Chronic Myeloid Leukemia Treatments
- Acute Lymphoblastic Leukemia research
- Chronic Lymphocytic Leukemia Research
- RNA and protein synthesis mechanisms
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Hematopoietic Stem Cell Transplantation
- Genomics and Chromatin Dynamics
- Parathyroid Disorders and Treatments
- Retinoids in leukemia and cellular processes
- Hemoglobinopathies and Related Disorders
- Peptidase Inhibition and Analysis
- Immune Cell Function and Interaction
- Lymphoma Diagnosis and Treatment
- Cardiovascular Function and Risk Factors
- Cell Adhesion Molecules Research
- Cardiac Valve Diseases and Treatments
- Immunodeficiency and Autoimmune Disorders
- Monoclonal and Polyclonal Antibodies Research
- HIV/AIDS drug development and treatment
Kumamoto University
2016-2025
The University of Tokyo
2014-2025
Tokyo National Hospital
2023-2025
National Cancer Center Hospital East
2023-2024
Toyohashi Municipal Hospital
2024
National Cancer Center
2024
Kumamoto University Hospital
2016-2023
Hiroshima University
2009-2019
Japanese Red Cross Nagoya Daiichi Hospital
2016-2018
Japanese Red Cross Nagoya Daini Hospital
2017-2018
Upon binding double-stranded DNA (dsDNA), cyclic GMP-AMP synthase (cGAS) is activated and initiates the cGAS-stimulator of IFN genes (STING)-type I interferon pathway. DEAD-box helicase 41 (DDX41) a helicase, mutations in DDX41 cause myelodysplastic syndromes (MDSs) acute myeloid leukemia (AML). Here, we show that DDX41-knockout (KO) cells have reduced type production after virus infection. Unexpectedly, activations cGAS STING are affected KO cells, suggesting functions upstream cGAS. The...
Comprehensive genomic profiling (CGP) is increasingly used as a clinical laboratory test and being applied to cancer treatment; however, standardization external quality assessments (EQA) have not been fully developed. This study performed cost-effective EQA proficiency tests (PT) for CGP testing among multiple institutions those belong the working group of Japan Association Clinical Laboratory Science (JACLS). revealed that preanalytical processes, such derived nucleic acids (NA) extraction...
Bcr-Abl kinase is known to reverse apoptosis of cytokine-dependent cells due cytokine deprivation, although it has been controversial whether chronic myeloid leukemia (CML) progenitors have the potential survive under conditions in which there are limited amounts cytokines. Here we demonstrate that early hematopoietic (Sca-1(+) c-Kit(+) Lin(-)) isolated from normal mice rapidly undergo absence In these cells, expression Bim, a proapoptotic relative Bcl-2 plays key role cytokine-mediated...
The eleven-nineteen leukemia (ENL) protein family, composed of ENL and AF9, is a common component 3 transcriptional modulators: AF4-ENL-P-TEFb complex (AEP), DOT1L-AF10-ENL (referred to as the DOT1L complex) polycomb-repressive 1 (PRC1). Each associates with chromatin via distinct mechanisms, conferring different properties including activation, maintenance, repression. mixed-lineage (MLL) gene often fuses AF10 family genes in leukemia. However, functional interrelationship among those...
Abstract Human papilloma virus (HPV)-associated oropharyngeal cancer (OPC) is an independent tumour type with regard to cellular, biological, and clinical features. The use of non-invasive biomarkers such as circulating DNA (ctDNA) may be relevant in early diagnosis eventually improve the outcomes patients head neck squamous cell carcinoma (HNSCC). Genome-wide discovery using RNA sequencing reduced representation bisulfite yielded 21 candidates for methylation-targeted genes. A verification...
Abstract Myeloid malignancies with DDX41 mutations are often associated bone marrow failure and cytopenia before overt disease manifestation. However, the mechanisms underlying these specific conditions remain elusive. Here, we demonstrate that loss of function impairs efficient RNA splicing, resulting in DNA replication stress excess R-loop formation. Mechanistically, binds to 5′ splice site (5′SS) coding coordinates splicing transcriptional elongation; prevents splicing-coupled transient...
Telaprevir is a potent inhibitor of hepatitis C virus (HCV) NS3-4A protease. However, the emergence drug-resistant strains during therapy serious problem, and susceptibility resistant to interferon (IFN), as well details mutant in vivo, not known. We previously established an infectious model HCV using human hepatocyte chimeric mice. Using this system we investigated biological properties mode mutants by ultra-deep sequencing technology. Chimeric mice were injected with serum samples...
Abstract Gene rearrangements generate MLL fusion genes, which can lead to aggressive leukemia. In most cases, fuses with a gene encoding component of the AEP (AF4 family/ENL family/P-TEFb) coactivator complex. MLL–AEP proteins constitutively activate their target genes immortalize haematopoietic progenitors. Here we show that and transcription through selectivity factor 1 (SL1), core pre-initiation complex (PIC) RNA polymerase I (RNAP1). The pSER domain AF4 family associates SL1 on chromatin...