A5055250823- Hematopoietic Stem Cell Transplantation
- Mesenchymal stem cell research
- Acute Myeloid Leukemia Research
- Immune Cell Function and Interaction
- Lung Cancer Research Studies
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- CAR-T cell therapy research
- Virus-based gene therapy research
- T-cell and B-cell Immunology
- Epigenetics and DNA Methylation
- Cancer Research and Treatments
- CRISPR and Genetic Engineering
- Immune cells in cancer
- Renal and related cancers
- Immunotherapy and Immune Responses
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cell Adhesion Molecules Research
- Blood Coagulation and Thrombosis Mechanisms
- Zebrafish Biomedical Research Applications
- Immune Response and Inflammation
- Platelet Disorders and Treatments
- Nuclear materials and radiation effects
- Catalytic Processes in Materials Science
- Hemophilia Treatment and Research
The University of Tokyo
2016-2025
Tokyo University of Science
2009-2025
Ibaraki University
2025
University of Tsukuba
2003-2024
University of Tsukuba Hospital
2024
Shizuoka Institute of Science and Technology
2000-2024
Saitama Prefectural Kumagaya Girls High School
2020-2024
Nagoya University
1998-2023
St. Marianna University School of Medicine
1999-2023
Osaka University of Pharmaceutical Sciences
2022-2023
In vitro generation of hematopoietic stem cells (HSCs) from induced pluripotent (iPSCs) has the potential to provide novel therapeutic approaches for replacing bone marrow (BM) transplantation without rejection or graft versus host disease. Hitherto, however, it proved difficult generate truly functional HSCs transplantable adult mice. Here, we demonstrate a unique in vivo differentiation system yielding engraftable mouse and human iPSCs teratoma-bearing animals combination with maneuver...
A specialized bone marrow microenvironment (niche) regulates hematopoietic stem cell (HSC) self-renewal and commitment. For successful donor-HSC engraftment, the niche must be emptied via myeloablative irradiation or chemotherapy. However, myeloablation can cause severe complications even mortality. Here we report that essential amino acid valine is indispensable for proliferation maintenance of HSCs. Both mouse human HSCs failed to proliferate when cultured in valine-depleted conditions. In...
ASXL1 mutations occur frequently in myeloid neoplasms and are associated with poor prognosis. However, the mechanisms by which mutant induces leukaemogenesis remain unclear. In this study, we report mutually reinforcing effects between a C-terminally truncated form of (ASXL1-MT) BAP1 promoting leukaemogenesis. expression results increased monoubiquitination ASXL1-MT, turn increases catalytic function BAP1. This hyperactive ASXL1-MT/BAP1 complex promotes aberrant differentiation...
Abstract Hematopoietic stem cells (HSCs) exhibit considerable cell-intrinsic changes with age. Here, we present an integrated analysis of transcriptome and chromatin accessibility aged HSCs downstream progenitors. Alterations in preferentially take place aging, which gradually resolve differentiation. Differentially open accessible regions (open DARs) are enriched for enhancers show enrichment binding motifs the STAT, ATF, CNC family transcription factors that activated response to external...
One of the central tasks stem cell biology is to understand molecular mechanisms that control self-renewal in cells. Several cytokines are implicated as crucial regulators hematopoietic cells (HSCs), but little known about intracellular signaling for HSC self-renewal. To address this issue, we attempted clarify how potential enhanced HSCs without adaptor molecule Lnk, Lnk-deficient mice expanded number >10-fold because their increased potential. We show Lnk negatively regulates by modifying...
Porous silicon carbide (SiC) membrane supports sintered at 1500°–1800°C were prepared by cold isostatic pressing (CIP) under different pressures and using amounts of alumina additive (0%–4%). The relationship between processing factors pore size microstructure was examined. Varying the sintering temperature, CIP pressure amount used found to be effective for controlling microstructure. particle support without increase with increasing temperature. This attributed surface diffusion....
Leukocyte mono-immunoglobulin (Ig)–like receptor 5 (LMIR5)/CD300b is a DAP12-coupled activating predominantly expressed in myeloid cells. The ligands for LMIR have not been reported. We identified T cell Ig mucin 1 (TIM1) as possible ligand LMIR5 by retrovirus-mediated expression cloning. TIM1 interacted only with among the family, whereas TIM4 well TIM1. Ig-like domain of bound to vicinity phosphatidylserine (PS)-binding site within Unlike its binding or TIM4, failed bind PS. did affect...
The lack of knowledge about the mechanism erythrocyte biogenesis through self-replication makes in vitro generation large quantities cells difficult. We show that transduction c-MYC and BCL-XL into multipotent hematopoietic progenitor derived from pluripotent stem gene overexpression enable sustained exponential glycophorin A+ erythroblasts, which we term immortalized (imERYPCs). In an inducible expression system, turning off enabled imERYPCs to mature with chromatin condensation reduced...
The discovery of induced pluripotent stem cells (iPSCs) has created promising new avenues for therapies in regenerative medicine. However, the tumorigenic potential undifferentiated iPSCs is a major safety concern clinical translation. To address this issue, we demonstrated efficacy suicide gene therapy by introducing inducible caspase-9 (iC9) into iPSCs. Activation iC9 with specific chemical inducer dimerization (CID) initiates caspase cascade that eliminates and tumors originated from We...