A5101461134- Ovarian cancer diagnosis and treatment
- Epigenetics and DNA Methylation
- Cancer-related gene regulation
- Endometriosis Research and Treatment
- Uterine Myomas and Treatments
- Estrogen and related hormone effects
- Ovarian function and disorders
- Reproductive Biology and Fertility
- Growth Hormone and Insulin-like Growth Factors
- Protein Kinase Regulation and GTPase Signaling
- RNA modifications and cancer
- Head and Neck Cancer Studies
- Gynecological conditions and treatments
- Hypothalamic control of reproductive hormones
- Sports injuries and prevention
- Endometrial and Cervical Cancer Treatments
- Neuroendocrine Tumor Research Advances
- Reproductive System and Pregnancy
- Renal and related cancers
- Lung Cancer Research Studies
- Sexual Differentiation and Disorders
- Cervical Cancer and HPV Research
- Hernia repair and management
- Hormonal and reproductive studies
- MRI in cancer diagnosis
Hiroshima Institute of Technology
2025
Hamamatsu University School of Medicine
2015-2024
Matsunami General Hospital
2013-2023
Osaka Dental University
2019
Hamamatsu University
2013-2018
Waseda University
2014-2018
Gifu Prefecture General Education Center
2018
Shizuoka Cancer Center
2016
Mejiro University
2014
Fujieda Municipal General Hospital
2013
Abstract Human papilloma virus (HPV)-associated oropharyngeal cancer (OPC) is an independent tumour type with regard to cellular, biological, and clinical features. The use of non-invasive biomarkers such as circulating DNA (ctDNA) may be relevant in early diagnosis eventually improve the outcomes patients head neck squamous cell carcinoma (HNSCC). Genome-wide discovery using RNA sequencing reduced representation bisulfite yielded 21 candidates for methylation-targeted genes. A verification...
Stimulated inositolphospholipid turnover has been proposed to be initiated and sustained by hydrolysis of phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2], which may replenished an enhanced flux (PtdIns) PtdIns 4-phosphate (PtdIns4P) PtdIns(4,5)P2. To determine whether there is continued resynthesis PtdIns(4,5)P2 in rat pituitary cells (GH3 cells) during stimulation thyrotropin-releasing hormone (TRH), we investigated the kinetics inositolphospholipids phosphatidic acid (PtdOH). In...
// Kiyoshi Misawa 1 , Daiki Mochizuki Atsushi Imai Shiori Endo Masato Mima Yuki Takeharu Kanazawa 2 Thomas E. Carey 3 Hiroyuki Mineta Department of Otolaryngology/Head and Neck Surgery, Hamamatsu University School Medicine, Shizuoka, Japan Jichi Medical University, Tochigi, Laboratory Head Cancer Biology, Michigan, Ann Arbor, MI, USA Correspondence to: Misawa, e-mail: kiyoshim@hama-med.ac.jp Keywords: tumor-suppressor genes, hypermethylation, head neck cancer, metastases, biomarker Received:...
The signaling pathway by which GnRH acts in peripheral tumors is distinct from that the anterior pituitary. We attempted to identify guanosine triphosphate (GTP)-binding protein (G protein) subtypes linked receptor genital tract tumor membranes. Surgically removed ovarian carcinomas and uterine leiomyosarcomas were screened for expression before plasma membrane isolation. G alpha i was detected immunoblotting of extracts with specific antibody pertussis toxincatalyzed ADP-ribosylation...
Background. Gonadotropin-releasing hormone (GnRH) analogs have been used in the therapy of endocrine-dependent cancers. The authors attempted to determine frequency with which Gn-RH receptor (Gn-RHR) is present gynecological Methods. Experiments were performed on gynecologic tumors that had surgically removed and their cloned cell lines. Gn-RHR was characterized by [3H]Gn-RH binding plasma membrane preparations. messenger ribonucleic acid determined reverse transcription-polymerase chain...
BACKGROUND Gonadotropin-releasing hormone (Gn-RH) receptor (Gn-RHR) has been demonstrated in epithelial ovarian carcinoma (Imai et al., Cancer 1994;74:2555-61). To examine whether Gn-RHR mediates direct antiproliferative effects, we attempted to determine stimulatory regulation by Gn-RH of phosphotyrosine phosphatase (PTP) activity plasma membranes isolated from samples. METHODS Surgically removed carcinomas were screened for expression prior membrane isolation. The level was observed by:...
<i>Background/Aims:</i> Recent clinical applications suggest a beneficial effect of gonadotropin-releasing hormone analog (GnRHa) as gonadal protector from chemotherapy-induced premature ovarian failure. This study aimed to determine cellular mechanisms involved in the protective action GnRHa against granulosa cell damage caused by doxorubicin. <i>Methods:</i> Granulosa cells were obtained ultrasound-guided follicular aspiration patients undergoing vitro...
The aim of this study was to clarify the epigenetic regulation ten eleven translocation protein (TET) family genes, which can provide insights into mechanisms tumorigenesis and risk disease recurrence in head neck squamous cell carcinoma (HNSCC). We generated methylation profiles TET1, TET2 TET3 genes tumor samples obtained from 233 patients with HNSCC; these included 57 hypopharynx, 44 larynx, 69 oral cavity, 63 oropharynx samples. mRNA expression promoter DNA TET were examined via...
Fas ligand induces cell death by means of apoptosis in a variety types when cross-linked with its natural receptor, Fas. GnRH receptor-bearing tumors undergo vivo and vitro agonists. To provide potential association the system antiproliferative signaling process we have evaluated regulation expression receptor-positive cloned lines known to substantial receptors. Surgically removed uterine endometrial carcinomas ovarian had been screened for receptor before analysis. protein was...
The efficacy of endoscopic screening for esophageal cancer in patients with hypopharyngeal remains controversial and its impact on prognosis has not been adequately discussed. We studied the use to detect by analyzing incidence, stage prognosis.We included 64 who received radical radiotherapy at our institute. Chromoendoscopic examinations Lugol dye solution were routinely performed after treatment cancer.Twenty-eight cancers detected 28 (41%) (18 synchronous 10 metachronous cancers). Of...
Improvements in cancer therapy have enabled further insight into the long-term effects of treatment, including highly prevalent gonadal failure. The focus treatment has been shifted to preservation fertility, which may be achieved by preventing ovarian toxicity. To this end, new molecular-targeted agents, monoclonal antibodies, developed and used a standard procedure for managing different cancers. However, prolonged antitumor activity these drugs cause emergence toxic effects. aim present...
The nordic hamstring exercise (NHE) is a dynamic lengthening that requires trunk and hip muscles activation. Thigh activation, specifically hamstring/quadriceps contractions has been previously examined during NHE. Trunk activity have not enough studied. aim of this study was to analyze Surface electromyography (EMG) kinematic data were collected Ten healthy men with the age range 21–36 years performed two sets repetitions downward upward motions each EMG fifteen knee collected. Muscle...
Purpose The aim of this study was to define somatostatin (SST) and receptor type 1 (SSTR1) methylation profiles for head neck squamous cell carcinoma (HNSCC) tumors at diagnosis follow up evaluate their prognostic significance value as a biomarker. Methods Gene expression measured by quantitative RT-PCR. Promoter status determined methylation-specific PCR (Q-MSP) in HNSCC. Results Methylation associated with transcription inhibition. SST 81% HNSCC tumor specimens significantly correlated...
Lysophosphatidic acid (LPA) mediates pleomorphic effects on multiple cell lineages, including an increased proliferative response of ovarian cancer cells both in vitro and vivo, at least part through the novel expression LPA receptors. Thus, hydrolysis is necessary to limit duration LPA's action types, cells. We determined principal mechanism by its regulation GnRH, which known have antiproliferative actions carcinomas. LPA-hydrolyzing activity membranes specimens was assessed measuring...
There is accumulating evidence that galanin receptors (GALRs) may be tumor suppressors in head and neck squamous cell carcinoma (HNSCC). Promoter methylation status gene expression were assessed a large panel of primary tumors, based on the hypothesis CpG hypermethylation might silence gene. Galanin was examined using reverse transcription-polymerase chain reaction (PCR). The promoter studied bisulfate sequencing methylation-specific PCR. UM-SCC-54 stably transfected to express galanin....