A5021959841- RNA modifications and cancer
- RNA Research and Splicing
- interferon and immune responses
- Immune Cell Function and Interaction
- RNA and protein synthesis mechanisms
- Immune cells in cancer
- Cytokine Signaling Pathways and Interactions
- DNA Repair Mechanisms
University of Saskatchewan
2021-2023
Upon binding double-stranded DNA (dsDNA), cyclic GMP-AMP synthase (cGAS) is activated and initiates the cGAS-stimulator of IFN genes (STING)-type I interferon pathway. DEAD-box helicase 41 (DDX41) a helicase, mutations in DDX41 cause myelodysplastic syndromes (MDSs) acute myeloid leukemia (AML). Here, we show that DDX41-knockout (KO) cells have reduced type production after virus infection. Unexpectedly, activations cGAS STING are affected KO cells, suggesting functions upstream cGAS. The...
DDX43 (DEAD-box helicase 43), also known as HAGE (helicase antigen gene), is a member of the DEAD-box protein family. It contains K homology (KH) domain in its N terminus, core C and flexible linker between. expression low or undetectable normal tissue, but overexpressed many tumors; therefore, it considered potential target molecule for cancer therapy. We, along with other groups, have shown that an ATP-dependent RNA DNA helicase, KH required ATPase unwinding activity. Electrophoretic...
Upon binding double-stranded (ds)DNA, cGAS is activated and initiates the cGAS-STING-type I interferon pathway; thus, availability of dsDNA ligand critical for activation. DDX41 a DEAD-box helicase mutations in cause myelodysplastic syndromes (MDS) acute myeloid leukemia (AML). Here, we found that DDX41-knockout (KO) human cells mice primary had reduced type production after viral infection, which consistent with previous reports. Unexpectedly, STING activation were affected KO cells,...