A5002703410- Retinal Diseases and Treatments
- Retinal and Macular Surgery
- Intraocular Surgery and Lenses
- Retinal Imaging and Analysis
- Glaucoma and retinal disorders
- Retinopathy of Prematurity Studies
- Retinal and Optic Conditions
- Pancreatic function and diabetes
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Retinal Development and Disorders
- DNA Repair Mechanisms
- Neurological Disorders and Treatments
- Facial Rejuvenation and Surgery Techniques
- RNA modifications and cancer
- Research on Leishmaniasis Studies
- Growth Hormone and Insulin-like Growth Factors
- Advanced Glycation End Products research
- Diabetes and associated disorders
- Cytokine Signaling Pathways and Interactions
- Pharmacological Effects and Toxicity Studies
- Metabolism, Diabetes, and Cancer
- Vascular Malformations Diagnosis and Treatment
- SARS-CoV-2 and COVID-19 Research
- Vascular Malformations and Hemangiomas
University of Utah
2024
University of Saskatchewan
2017-2023
Medical College of Wisconsin
2012-2022
Regional Medical Research Centre
2021-2022
Indian Council of Medical Research
2022
Retina Associates
2016-2021
Sanjay Gandhi Post Graduate Institute of Medical Sciences
2006-2018
Saskatchewan Health
2017
National Center for Disease Control
2017
Government of India
2017
To identify amacrine cells that are vulnerable to degeneration during the early stages of diabetes.Whole retinas from streptozotocin (STZ)-diabetic rats and Ins2(Akita) mice were fixed in paraformaldehyde. Apoptotic retina quantified using terminal dUTP nick-end labeling (TUNEL) active caspase-3 (CM-1) immunohistochemistry. Immunohistochemical markers for choline acetyltransferase (ChAT) tyrosine hyroxylase (TH) also used quantify populations Ins2Akita mouse retinas.The number TUNEL-positive...
Diabetic retinopathy is characterized by early onset of neuronal cell death. We previously showed that insulin mediates a prosurvival pathway in retinal neurons and normal retina expresses highly active basal receptor/Akt signaling stable throughout feeding fasting. Using the streptozotocin-induced diabetic rat model, we tested hypothesis diabetes diminishes receptor concomitantly with increased diabetes-induced apoptosis. The expression, phosphorylation status, and/or kinase activity...
Outbreaks of unexplained illness frequently remain under-investigated. In India, outbreaks an acute neurological with high mortality among children occur annually in Muzaffarpur, the country's largest litchi cultivation region. 2014, we aimed to investigate cause and risk factors for this illness.In hospital-based surveillance nested age-matched case-control study, did laboratory investigations assess potential infectious non-infectious causes illness. Cases were aged 15 years or younger who...
Non-invasive renal signatures can help in serial monitoring of diabetic patients. We tested whether urinary exosomal (UE) microRNA (miR) analysis could non-invasively predict pathology rats during the course diabetes. Diabetes mellitus (DM) was induced male Wistar by a single intraperitoneal injection streptozotocin (STZ, 50 mg/kg body weight). Non-diabetic control (CTRL) were injected with vehicle. Insulin (INS) treatment (5U/d, s.c.) provided to 50% DM rats. Urine samples collected at...
Upon binding double-stranded DNA (dsDNA), cyclic GMP-AMP synthase (cGAS) is activated and initiates the cGAS-stimulator of IFN genes (STING)-type I interferon pathway. DEAD-box helicase 41 (DDX41) a helicase, mutations in DDX41 cause myelodysplastic syndromes (MDSs) acute myeloid leukemia (AML). Here, we show that DDX41-knockout (KO) cells have reduced type production after virus infection. Unexpectedly, activations cGAS STING are affected KO cells, suggesting functions upstream cGAS. The...
Nearly all renal tubular epithelial cells express insulin receptor. The receptor in the distal tubule appears to modulate BP, but role of proximal is unknown. Here, we selectively knocked out from tubules mice. Western blotting confirmed a two- three-fold reduction cortical homogenate receptor-β among knockout mice compared with wild-type littermates. Young exhibited mildly diabetic phenotype, evidenced by higher fasting plasma glucose levels than Assessments hyperinsulinemic-euglycemic...
The relationship between fat and bone mass at distinct trabecular cortical skeletal compartments in a high-fat diet (HFD) model was studied. For this, C57BL/6 mice were assigned to four groups of eight animals each. Two groups, each males females, received standard chow while the remaining other two HFD for period 10 weeks. Male on heavier gained more weight (15·8 %; P < 0·05) v. those control or when compared with female rats fed HFD. We observed an increased lipid profile both...
Diabetic retinopathy is the leading cause of blindness in working age persons. Targeted studies have uncovered several components pathophysiology disease without unveiling basic mechanisms. This study describes use complementary proteomic and genomic discovery methods that revealed proteins crystallin superfamily are increased dramatically early diabetic retinopathy. Orthogonal confirmed amplitude up-regulation greater than other changes described so far A detailed time course during...
Diabetes pathology derives from the combination of hyperglycemia and hypoinsulinemia or insulin resistance leading to diabetic complications including neuropathy, nephropathy retinopathy. Diabetic retinopathy is characterized by numerous retinal defects affecting vasculature neuro-retina, but relative contributions loss signaling have never been directly compared. In this study we tested hypothesis that increased glycemic normalization would exert differential effects on cell survival...
Venipuncture is the most common painful event for a hospitalized child. We evaluated efficacy of balloon inflation attenuating venipuncture pain in children. Seventy-five pediatric patients aged 6-12 yr, ASA physical status I-II, either sex, undergoing elective surgery were included this prospective and randomized study. Patients randomly divided into 3 equal groups 25 each; Group I (control), II (distraction) pressed rubber ball, III (balloon) inflated balloon. A manual venous occlusion was...
In Brief Purpose: To evaluate outer retinal structural abnormalities in patients with visual deficits after closed-globe blunt ocular trauma. Methods: Nine subjects complaints trauma were examined between 1 month and 6 years Spectral domain optical coherence tomography was used to assess the architecture, whereas adaptive optics scanning light ophthalmoscopy analyze photoreceptor mosaic integrity. Results: Visual ranged from central scotomas decreased acuity. defects included focal foveal...
The K-homology (KH) domain is a nucleic acid-binding present in many proteins. Recently, we found that the DEAD-box helicase DDX43 contains KH its N-terminus; however, function remains unknown. Here, purified recombinant protein and it prefers binding ssDNA ssRNA. Electrophoretic mobility shift assay NMR revealed favors pyrimidines over purines. Mutational analysis showed GXXG loop involved pyrimidine binding. Moreover, an alanine residue adjacent to critical for Systematic evolution of...
Abstract Statins, 3-hydroxy-3-methyl-glutaryl-coenzyme A reductase inhibitors have been shown to improve diabetic nephropathy. However, whether they provide protection via Histone deacetylases (HDAC) inhibition is not clear. We conducted a comparative evaluation of Atorvastatin (AT) versus the non-statin cholesterol-lowering drug, Ezetimibe (EZT) on severity Streptozotocin-treated male Wistar rats were fed cholesterol-supplemented diet and gavaged daily with vehicle, AT or EZT. Control...
Background Few prospective data exist on incidence of post kala-azar dermal leishmaniasis (PKDL) and visceral (VL) relapse after different treatment regimens. Methodology/Principal findings A Phase IV trial included 1761 VL patients treated between 2012–2014 with single dose AmBisome (SDA; N = 891), miltefosine-paromomycin (Milt-PM; n 512), or AmBisome-miltefosine (AmB-Milt; 358). Follow-up for PKDL was scheduled 6, 12 24 months treatment, lasting until 2017. Patients lesions consistent were...
JavaScript is a client side scripting language which used for providing dynamic attributes and features to the HTML webpages .JavaScript was developed supporting browser with feature of asynchronous communication, controlling user interaction web page components.An just static page.JavaScript can make website more interactive userfriendliness, helps easy navigation provides interactivity.Based on there are many frameworks also added new server scripting.JavaScript has emerged so much in...
Background. Matrix metalloproteinases (MMPs), especially basement membrane-degrading MMP-9 and MMP-2, play an important role in immune-mediated tissue destruction of the allograft by allowing influx leucocytes mononuclear cells into graft. The present study was, therefore, undertaken to investigate association functional polymorphisms (836A>G, 1721G>C 2003G>A) MMP-2 (−735C>T) genes with risk rejection renal transplant recipients North India. Methods. Three hundred six categorized 228...
Background In 2010, WHO recommended the use of new short-course treatment regimens in kala-azar elimination efforts for Indian subcontinent. Although phase 3 studies have shown excellent results, there remains a lack evidence on wider population and safety effectiveness these under field conditions. Methods This was an open label, prospective, non-randomized, non-comparative, multi-centric trial conducted within public health facilities two highly endemic districts specialist referral centre...
DDX43 (DEAD-box helicase 43), also known as HAGE (helicase antigen gene), is a member of the DEAD-box protein family. It contains K homology (KH) domain in its N terminus, core C and flexible linker between. expression low or undetectable normal tissue, but overexpressed many tumors; therefore, it considered potential target molecule for cancer therapy. We, along with other groups, have shown that an ATP-dependent RNA DNA helicase, KH required ATPase unwinding activity. Electrophoretic...