A5022260018- Sperm and Testicular Function
- Acute Myeloid Leukemia Research
- Genetic and phenotypic traits in livestock
- Epigenetics and DNA Methylation
- Reproductive Biology and Fertility
- Livestock and Poultry Management
- Immune Cell Function and Interaction
- Chronic Myeloid Leukemia Treatments
- Animal Nutrition and Physiology
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Evolution and Paleontology Studies
- Bat Biology and Ecology Studies
- Comparative Animal Anatomy Studies
- Animal Ecology and Behavior Studies
- TGF-β signaling in diseases
- Chronic Lymphocytic Leukemia Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Cancer, Hypoxia, and Metabolism
- Animal Genetics and Reproduction
- Mitochondrial Function and Pathology
- Physiological and biochemical adaptations
- Effects and risks of endocrine disrupting chemicals
- Erythrocyte Function and Pathophysiology
- Wildlife Ecology and Conservation
- Parasitic Diseases Research and Treatment
Kanazawa Medical University
2020-2025
Shimane University
2025
Tokyo University of Pharmacy and Life Sciences
2017-2024
Tokyo University of Agriculture
1996-2024
Ritsumeikan University
2024
Life Science Institute
2024
Onomichi General Hospital
2022
Cincinnati Children's Hospital Medical Center
2013-2020
The University of Tokyo
2003-2020
Kyoto University Hospital
2010-2020
In the mouse, definitive endoderm is derived from epiblast during gastrulation, and, at early organogenesis stage, forms primitive gut tube, which gives rise to digestive tract, liver, pancreas and associated visceral organs. The transcription factors, Sox17 (a Sry-related HMG box factor) its upstream Mixer (homeobox Casanova novel Sox factor), have been shown function as determinants in Xenopus zebrafish, respectively. However, whether mammalian orthologues of these genes are also involved...
The different mRNA isoforms of the mouse Sox17 gene were isolated from adult testis cDNAs. One form (referred to as Sox17) encodes an Sry-related protein 419 amino acids containing a single high mobility group box near NH2-terminus, while other t-Sox17) shows unique isoform with partial deletion HMG region. Analysis genomic DNA revealed that these two produced at least by alternative splicing exon corresponding 5' untranslated region and NH2-terminal 102 acids. RNA analyses in began...
Different H-2 congenic strains of mice were immunized with a P. falciparum sporozoite vaccine currently being tested in humans, or different segments the molecule. Specific IgG production lymph node cell proliferation response to antigens was then determined. Only four seven (representing three eight possible class II restriction molecules) responded vaccine. Of those molecules, only one, I-Ab, associated malaria-encoded T epitope [contained within NP(NANP)3NA], while other two molecules (E...
Abstract Sickle cell disease (SCD) is a heritable disorder caused by β-globin gene mutations. Induction of fetal γ-globin an established therapeutic strategy. Recently, epigenetic modulators, including G9a inhibitors, have been proposed as agents. However, the molecular mechanisms whereby these small molecules reactivate remain unclear. Here we report development highly selective and non-genotoxic inhibitor, RK-701. RK-701 treatment induces globin expression both in human erythroid cells...
Inflammatory stimuli cause a state of emergency myelopoiesis leading to neutrophil-like monocyte expansion. However, their function, the committed precursors, or growth factors remain elusive. In this study we find that Ym1+Ly6Chi monocytes, an immunoregulatory entity arise from progenitors neutrophil 1 (proNeu1). Granulocyte-colony stimulating factor (G-CSF) favors production monocytes through previously unknown CD81+CX3CR1lo precursors. GFI1 promotes differentiation proNeu2 proNeu1 at cost...
There are two types of the activin-binding protein follistatin (FS), FS-288 and FS-315. These result from alternative splicing mRNA. exhibits high affinity for cell-surface heparan sulfate proteoglycans, whereas FS-315 shows low affinity. To understand physiological role cell-associated FS, we investigated binding activin to FS its behavior on cell surface using primary cultured rat pituitary cells. Affinity cross-linking experiments using125I-activin A demonstrated that bound cells via as...
Bisphenol‐A Affects Spermatogenesis in the Adult Rat Even at a Low Dose: Motoharu Sakaue, et al. Environmental Health Sciences Division, National Institute for Studies— (BPA), xenobiotic estrogenic compound widely used as plastics monomer, has been suspected to have so‐called low dose effect on reproductive system when administered transplacentally. In present study, we investigated possible low‐dose effects of BPA spermatogenesis adult rats. Male rats (13 weeks old; W13) were administrated...
Summary In this study, we analysed the mitochondrial DNA D‐loop region of Japanese native chickens to clarify their phylogenetic relationships, possible maternal origin and routes introduction into Japan. Seven haplogroups (Types A–G) were identified. Types A–C observed in Jidori, Shokoku related breeds. However, Type C was absent Shokoku, which introduced from China, while most Indonesian included haplogroup. D–G Shamo E had a close genetic relationship with Chinese chickens. Our results...
Granulopoiesis is tightly regulated to meet host demands during both "steady-state" and "emergency" situations, such as infections. The transcription factor CCAAT/enhancer binding protein β (C/EBPβ) plays critical roles in emergency granulopoiesis, but the precise developmental stages which C/EBPβ required are unknown. In this study, a novel flow cytometric method was developed that successfully dissected mouse bone marrow cells undergoing granulopoiesis into five distinct subpopulations...
In the prespermatogenesis period, male germ cells (gonocytes) begin to reproliferate and move basement membrane of seminiferous tubule. Although these two events—reproliferation relocation—are important for establishment spermatogenesis, they have not been greatly analyzed both in a mechanical an endocrine or paracrine aspect. this study, relationship between reproliferation relocation gonocytes was examined, using thymidine analog bromodeoxyuridine (BrdU) labeling method transmission...
We have previously demonstrated that melatonin protects against ischemia/reperfusion-induced oxidative damage to mitochondria in the fetal rat brain. The purpose of present study was evaluate effects maternally administered on placental and growth restriction rats. utero-ovarian arteries were occluded bilaterally for 30 min rats day 16 pregnancy induce ischemia. Reperfusion achieved by releasing occlusion restoring circulation. Melatonin solution (20 microg/mL) or vehicle alone orally during...
To identify target cells of prolactin (PRL) in the male gonad, expression receptor (PRL-R) mRNA adult rat testes was investigated by situ hybridization using a digoxigenin-labeled cRNA probe. We also PRL binding to testicular vitro. Signals for PRL-R were detected not only interstitial but spermatogenic cells. Although reaction positive all phases spermatogonia and spermatocytes, it disappeared early round spermatids. No signals elongated spermatids or spermatozoa. The signal intensity...
The pathogenesis of non-alcoholic fatty liver disease (NAFLD) is incompletely understood. Kupffer cells (KCs), phagocytic liver-resident macrophages, provide a protective barrier against egress endotoxin from the portal to systemic circulation. It not known if KC function impaired in NAFLD. Super-paramagnetic iron oxide (SPIO) magnetic resonance imaging comparative technology dependent on function.To evaluate uptake function, patients and experimental animals with NAFLD, using SPIO.Abdominal...
Myelodysplastic syndromes (MDS) are heterogeneous hematopoietic disorders that incurable with conventional therapy. Their incidence is increasing global population aging. Although many genetic, epigenetic, splicing, and metabolic aberrations have been identified in patients MDS, their clinical features quite similar. Here, we show hypoxia-independent activation of hypoxia-inducible factor 1α (HIF1A) signaling both necessary sufficient to induce dysplastic cytopenic MDS phenotypes. The HIF1A...
ABSTRACT Aim/Introduction Senescence is a key driver of age‐related kidney dysfunction, including diabetic disease. Oxidative stress activates cellular senescence, induces abnormal glycolysis, and associated with pyruvate kinase muscle isoform 2 (PKM2) dysfunction; however, the mechanisms linking PK activation to senescence have not been elucidated. We hypothesized that PKM2 by TEPP‐46 could suppress oxidative stress‐induced renal tubular cell injury senescence. Materials Methods To...
We first detected the mRNA expression of follistatin and three subunits inhibin/activin in rat pancreatic islets by reverse transcription-polymerase chain reaction (RT-PCR). Immunohistochemistry using anti-follistatin serum (against residues 123-134) revealed that was localized only insulin-producing B cells. Although beta A subunit detectable islets, immunostainable cell types were completely different with two antisera, i.e. anti-beta (1-10)-Tyr stained cells, while (87-99)...
We have used immunohistochemistry to localize follistatin/activin-binding protein in adult male and female rats. A polyclonal antibody directed against a follistatin peptide (residues 123-134) was as specific immunologic probe. Intense immunoreactivity evident spermatogenic cells of seminiferous tubules the testis. The predominant staining nuclei spermatocytes spermatids, but no immune reaction observed spermatogonia or spermatozoa. Moderate detected Leydig cells. Sertoli were...
Mucinous tubular and spindle cell carcinoma (MTSCC) of the kidney generally shows low nuclear grade. MTSCC with high grade is relatively rare. In this article, we report two cases Fuhrman 3. One case occurred in a 57-year-old Japanese female second 49-year-old Caucasian female. Histologically, tumors were composed neoplastic cells cuboidal or columnar morphology, The myxoid stroma was also observed. This positive for Alcian blue stain. Immunohistochemically, both AMACR, but negative CD10 RCC...
Multiple myeloma (MM) is a malignant neoplasm of plasma cells. Although new molecular targeting agents against MM have been developed based on the better understanding underlying pathogenesis, still remains an incurable disease. We previously demonstrated that β-catenin, downstream effector in Wnt pathway, potential target using RNA interference vivo experimental mouse model. In this study, we screened library more than 100 000 small-molecule chemical compounds for novel Wnt/β-catenin...
SET domain containing 2 (Setd2), encoding a histone methyltransferase, is associated with many hematopoietic diseases when mutated. By generating novel exon 6 conditional knockout mouse model, we describe an essential role of Setd2 in maintaining the adult stem cells. Loss results leukopenia, anemia, and increased platelets accompanied by hypocellularity, erythroid dysplasia, mild fibrosis bone marrow. mice show significantly decreased progenitor cells except for progenitors. fail to...