A5069620625- Lysosomal Storage Disorders Research
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Sperm and Testicular Function
- Reproductive Biology and Fertility
- Trypanosoma species research and implications
- Cellular transport and secretion
- Monoclonal and Polyclonal Antibodies Research
- Animal Genetics and Reproduction
- Mast cells and histamine
- Cancer Cells and Metastasis
- Biochemical and Molecular Research
- Muscle Physiology and Disorders
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Neurogenesis and neuroplasticity mechanisms
- Immune cells in cancer
- Pluripotent Stem Cells Research
- Studies on Chitinases and Chitosanases
- Cell Adhesion Molecules Research
- Chemokine receptors and signaling
- RNA Interference and Gene Delivery
- Biomedical Research and Pathophysiology
- Urologic and reproductive health conditions
- Photodynamic Therapy Research Studies
- HER2/EGFR in Cancer Research
Tokyo Metropolitan Institute of Medical Science
2005-2023
Meiji Pharmaceutical University
2009-2011
Japan Science and Technology Agency
2005-2007
Stony Brook University
2000
Cornell University
1998-1999
Kettering University
1999
Memorial Sloan Kettering Cancer Center
1998
Osaka University
1982-1995
Osaka Medical and Pharmaceutical University
1993
In the mouse, definitive endoderm is derived from epiblast during gastrulation, and, at early organogenesis stage, forms primitive gut tube, which gives rise to digestive tract, liver, pancreas and associated visceral organs. The transcription factors, Sox17 (a Sry-related HMG box factor) its upstream Mixer (homeobox Casanova novel Sox factor), have been shown function as determinants in Xenopus zebrafish, respectively. However, whether mammalian orthologues of these genes are also involved...
In order to define the role of Kit ligand (KL) growth factor encoded at mouse steel (SI) locus in spermatogenesis, we have examined its production Sertoli cells. As a measure KL bioactivity, ability support proliferation and maintenance mast cells was used co-culture with primary On sertoli derived from +/+ Wv/Wv mice, proliferated were supported for more than 2 weeks, but not W/Wv contrast, SId/SId mice could cell under similar conditions. The supportive effect required close-range...
Hypoglycosylation and reduced laminin-binding activity of α-dystroglycan are common characteristics dystroglycanopathy, which is a group congenital limb-girdle muscular dystrophies. Fukuyama-type dystrophy (FCMD), caused by mutation in the fukutin gene, severe form dystroglycanopathy. A retrotransposal insertion seen almost all cases FCMD. To better understand molecular pathogenesis dystroglycanopathies to explore therapeutic strategies, we generated knock-in mice carrying mouse ortholog....
Membrane growth factors that are processed to produce soluble ligands may function both as and membrane factors. The factor Kit-ligand (KL), the ligand of Kit receptor tyrosine kinase, is encoded at Sl locus, mice carrying mutations have defects in hematopoiesis, gametogenesis, melanogenesis. Two alternatively spliced KL transcripts encode two cell-associated protein products, KL-1 KL-2. KL-2 lacks major proteolytic cleavage site for generation KL, thus representing a more stable form KL. We...
Human beta-hexosaminidase A (HexA) is a heterodimeric glycoprotein composed of alpha- and beta-subunits that degrades GM2 gangliosides in lysosomes. gangliosidosis lysosomal storage disease which an inherited deficiency HexA causes the accumulation gangliosides. In order to prepare large amount for treatment based on enzyme replacement therapy (ERT), recombinant was produced methylotrophic yeast Ogataea minuta instead mammalian cells, are commonly used produce enzymes ERT. The problem...
Summary This study assessed the effect of growth factors on testicular germ cell differentiation in vitro . Testicular fragments experimentally prepared cryptorchid testes adult mice were cultured for 9 days serum‐free media containing various concentrations IGF‐I, TGF‐α, FGF, and PDGF. Their histology was then examined under a light microscope. Each type mitotic seminiferous tubules counted per 1000 Sertoli cells. IGF‐I at concentration 10 ng/ml induced maximal A spermatogonia. TGF‐α...
Testicular cells composed mostly of germ and immature Sertoli from neonatal mice 2 5 days old were cultured to investigate germ-cell proliferation mediated by the c-kit receptor. The addition antibody block interaction receptor with its ligand inhibited spermatogonia 5-day-old in a dose-dependent manner, but not that 2-day-old mice. anti-c-kit ACK2 monoclonal also mutant Sld/Sld Wo/Wo results indicate c-kit-positive type A testes require steel factor (kit ligand) for their proliferation,...
Recent studies have revealed an association between post-translational modification of α-dystroglycan (α-DG) and certain congenital muscular dystrophies known as secondary α-dystroglycanopathies (α-DGpathies). Fukuyama-type dystrophy (FCMD) is classified a α-DGpathy because the responsible gene, fukutin, putative glycosyltransferase for α-DG. To investigate pathophysiology α-DGpathies, we profiled gene expression in skeletal muscle from FCMD patients. cDNA microarray analysis quantitative...
Mammalian eukaryotic translation initiation factor 3 (eIF3) is the largest complex of factors. The eIF3 comprised thirteen subunits, which are named eIF3a to m in most multicellular organisms. eIF3e gene locus one frequent integration sites mouse mammary tumor virus (MMTV), induces tumors mice. MMTV-integration events result expression C-terminal-truncated proteins, leading formation. We have shown that formation can be partly caused by activation hypoxia-inducible 2α. To investigate...
The effect of epidermal growth factor (EGF) on testicular germ cell differentiation was investigated. Testicular fragments from surgically prepared cryptorchid testes adult mice were cultured for 9 days in serum-free media containing various concentrations EGF. Histological sections testis examined under a light microscope and each type mitotic the seminiferous tubules counted per 1000 Sertoli cells. EGF at ranging 100 to 200 ng/ml induced A spermatogonia. observed maximal stimulatory...
With the aim of developing more stable Gd(III)-porphyrin complexes, two types ligands 1 and 2 with carboxylic acid anchors were synthesized. Due to N-substituted pyridyl cation attached porphyrin core, these highly water-soluble formed corresponding Gd(III) chelates, Gd-1 Gd-2. was sufficiently in neutral buffer, presumably due preferred conformation carboxylate-terminated connected nitrogen meta position group helping stabilize complexation by center. 1H NMRD (nuclear magnetic relaxation...
We isolated a cDNA encoding novel glucuronyltransferase, designated GlcAT-D, involved in the biosynthesis of HNK-1 carbohydrate epitope from rat embryo by degenerate polymerase chain reaction method. The new sequence revealed an open reading frame coding for protein 324 amino acids with type II transmembrane topology. acid GlcAT-D displayed 50.0% identity to GlcAT-P, which is on glycoproteins. Expression COS-7 cells resulted formation cell surface. enzyme expressed transferred glucuronic...
The effect of white-spotting (W) mutations on differentiation testicular germ cells was investigated by using experimental cryptorchidism and its surgical reversal. All mutant mice used in this study (Wv/+, Wsh/+, Wf/+ Wf/Wf) showed normal fertility well-ordered spermatogenesis, as congenic +/+ mice. In the cryptorchid testis, which contains only type A spermatogonia cells, number proliferative activity were comparable to On other hand, reversal testis mutants resulted impaired regenerative...
<i>Pax6</i> is a transcription factor involved in brain patterning and neurogenesis. Expression of<i>Pax6</i> specifically observed the developing cerebral cortex, where Lewis x epitope that thought to play important roles cell interactions colocalized. Here we examined whether<i>Pax6</i> regulates localization of using<i>Pax6</i> mutant rat embryos. The disappeared activity α1,3-fucosyltransferase, which catalyzed last step biosynthesis, drastically decreased cortex as compared with wild...