A5077765371- Immune Response and Inflammation
- Allergic Rhinitis and Sensitization
- Asthma and respiratory diseases
- Voice and Speech Disorders
- Food Allergy and Anaphylaxis Research
- Carbohydrate Chemistry and Synthesis
- Airway Management and Intubation Techniques
- Lysosomal Storage Disorders Research
- NF-κB Signaling Pathways
- Mast cells and histamine
- Fungal and yeast genetics research
- T-cell and B-cell Immunology
- Epigenetics and DNA Methylation
- Biofuel production and bioconversion
- Glutathione Transferases and Polymorphisms
- Tracheal and airway disorders
- Dermatology and Skin Diseases
- Trypanosoma species research and implications
- Glycosylation and Glycoproteins Research
- Cancer-related Molecular Pathways
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Ubiquitin and proteasome pathways
- Spinal Dysraphism and Malformations
- Endometriosis Research and Treatment
Ehime University
2010-2025
Kanazawa Medical University
2014-2024
Kanazawa Medical University Hospital
2022
National Cancer Center Hospital East
2020
Kaohsiung Chang Gung Memorial Hospital
2015-2017
Chang Gung University
2015-2017
Hiroshima University
2006-2016
Kyoto Prefectural University of Medicine
2016
National Institute of Advanced Industrial Science and Technology
2002-2013
Kyoto University
2008-2012
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is an energy metabolism-related enzyme in the glycolytic pathway. Recently, it has been reported that GAPDH other physiological functions, such as apoptosis, DNA repair and autophagy. Some vitro studies have indicated immunological aspects of function, although there no definite study discussing advantage a therapeutic target. Here, we show anti-inflammatory function by using lipopolysaccharide (LPS)-induced, sepsis-related severe acute lung...
Human beta-hexosaminidase A (HexA) is a heterodimeric glycoprotein composed of alpha- and beta-subunits that degrades GM2 gangliosides in lysosomes. gangliosidosis lysosomal storage disease which an inherited deficiency HexA causes the accumulation gangliosides. In order to prepare large amount for treatment based on enzyme replacement therapy (ERT), recombinant was produced methylotrophic yeast Ogataea minuta instead mammalian cells, are commonly used produce enzymes ERT. The problem...
In four yeast strains, Ogataea minuta, Candida parapolymorpha, Pichia anomala and Zygosaccharomyces rouxii, we identified endo-β-N-acetylglucosaminidase (ENGase) homologous sequences by database searches; in each of the species, a corresponding enzyme activity was also confirmed crude cell extract obtained from strain. The O. minuta ENGase (Endo-Om)-encoding gene directly amplified genomic DNA sequenced. Endo-Om-encoding contained 2319-bp open-reading frame; deduced amino acid sequence...
A mammalian-like sugar moiety was created in glycoprotein by Saccharomyces cerevisiae combination with bacterial α-mannosidase to produce a more economic enzyme replacement therapy for patients Fabry disease. We introduced the human α-galactosidase (α-GalA) gene into an S. mutant that deficient outer chains of N-linked mannan. The recombinant α-GalA contained both neutral (Man8GlcNAc2) and acidic ([Man-P]1–2Man8GlcNAc2) chains. Because efficient incorporation lysosomes cells requires...
Yeast cells producing mammalian-type N-linked oligosaccharide show severe growth defects and the decreased protein productivity because of disruption yeast-specific glycosyltransferases. This in engineered yeast strains is an obstacle to development efficient glycoprotein production yeast. For economic effective synthesis such therapeutic glycoproteins yeast, appropriate highly desirable. We applied a novel mutagenesis technique that utilized proofreading-deficient DNA polymerase δ variant...
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is an energy metabolism-related enzyme, which generates NADH in glycolysis. Our previous study revealed a novel role of exogenous GAPDH the amelioration lipopolysaccharide (LPS)-induced sepsis-related, severe acute lung injury (ALI) mice. Here, we show effect extracellular on physiological functions macrophages, play important onset sepsis and ALI. has no cell viability, while it strongly suppressed adhesion, spreading, phagocytic function...
We tested the effect of oral administration fermented sake lees with lactic acid bacteria (FESLAB) on a murine model allergic rhinitis upon immunization and nasal sensitization ovalbumin (OVA). used Lactobacillus paracasei NPSRIk-4 (isolated from lees), L. brevis NPSRIv-8 (from milk) as starter strains to produce FESLAB. Oral FESLAB resulted in development significantly fewer sneezing symptoms than those seen sham control animals given sterile water. also found that suppressed...
In the course of identifying molecular mechanism that is related to strong cell-cell adhesion in stratified structures squamous epithelium, calmodulin-like protein 5 (CALML5) was identified as a spinous structure-associated by producing monoclonal antibodies with use crude intercellular portion tissue an immunogen and subsequent morphologic screening. By electrophoretic mobility shift assay (EMSA) series mutagenesis studies, two transcription factors, ZNF750 KLF4, binding line CALML5 gene...
Summary We previously demonstrated the immunosuppressive activity of anti‐histone H1 autoantibody induced in experimental and clinical liver allograft tolerance. This study aimed to explore immunological aspects injury by concanavalin A (Con A). To establish a Con A‐hepatitis model, 20 mg/kg was intravenously injected into rats, after which function histopathological analyses were performed. In this transiently sera during natural recovery stage, 3–7 days injection. evaluate therapeutic...
Histones play important roles in both host defenses and inflammation related to microbial infection. A peptide mimotope (SSV) was identified from a novel histone H1 monoclonal antibody (16G9 mAb) that shown inhibit the mixed lymphocyte reaction. In present study, an anti-SSV producing hybridoma established. We investigated effects of SSV mAb mouse acute model induced by intraperitoneal injection lipopolysaccharide (LPS).SSV generated characterized. Mice were treated with or control IgG prior...
While the rapid decrease in estrogen is well known as main cause of postmenopausal osteoporosis women, precise pathogenesis senile elderly regardless gender largely unknown. The age-related epigenetic regulation receptor activator NF-κB (RANK) gene expression was investigated with use a high-passaged mouse osteoclast progenitor cell line, RAW264.7, an
Mineralogically investigated were massive sulfide samples of chimneys and mounds collected during the "SHINKAI 6500" dives (Dive 562 Dive 567) from two basalt-hosted hydrothermal ore deposits, being at a short distance about 1 km, occurring in eastern end Yaeyama Central Graben, Southern Okinawa Trough. In both sample series (#562- #567- series), minerals are dominated by low-iron sphalerite with small amounts chalcopyrite galena. Sphalerite #567-series shows somewhat wide compositional...
Nuclear antigens are known to trigger off innate and adaptive immune responses. Recent studies have found that the complex of nucleic acids core histones derived from damaged cells may regulate allergic However, no fundamental study has been performed concerning role linker histone H1 in mast cell-mediated type I hyperreactivity. In this study, we explored impact on responses both vitro vivo. course a bona-fide experimental allergen sensitization model upon co-injection with alum adjuvant,...
Elevation of glutamate, an excitatory amino acid, during inflammation and injury plays a crucial role in the reception transmission sensory information via ionotropic metabotropic receptors. This study aimed to investigate mechanisms underlying biphasic effects glutamate mGlu5 receptor activation on responses noxious heat.We assessed intraplantar quisqualate, non-selective agonist, heat mechanical pain behaviours mice. In addition, quisqualate intracellular calcium response membrane currents...
Since epigenetic modifications differ from cell to cell, detecting the DNA methylation status of individual cells is requisite. Therefore, it important conduct "morphology-based epigenetics research", in which sequence-specific observed while maintaining tissue architecture. Here we demonstrate a novel histochemical technique that efficiently shows presence single methylated cytosine sequence-dependent manner by applying ICON (interstrand complexation with osmium for nucleic acids) probes....