A5101592326- Glycosylation and Glycoproteins Research
- Spine and Intervertebral Disc Pathology
- Carbohydrate Chemistry and Synthesis
- Immune Cell Function and Interaction
- Pineapple and bromelain studies
- T-cell and B-cell Immunology
- Musculoskeletal pain and rehabilitation
- Cellular transport and secretion
- Spinal Fractures and Fixation Techniques
- Porphyrin and Phthalocyanine Chemistry
- Immunotherapy and Immune Responses
- Banana Cultivation and Research
- Acute Myeloid Leukemia Research
- Anesthesia and Pain Management
- Satellite Communication Systems
- Analytical Chemistry and Chromatography
- Galectins and Cancer Biology
- Cervical and Thoracic Myelopathy
- Inflammasome and immune disorders
- Acute Lymphoblastic Leukemia research
- Plant tissue culture and regeneration
- Monoclonal and Polyclonal Antibodies Research
- Chemical Synthesis and Analysis
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Phenothiazines and Benzothiazines Synthesis and Activities
Tokushima University
2014-2025
Okinawa Prefectural Agricultural Research Center
2005-2024
Toranomon Hospital
2022-2024
Shizuoka University
2024
Nagoya University
1986-2023
Geological Survey of Japan
2021
RIKEN Center for Brain Science
2008-2020
RIKEN
2008-2020
Okayama Red Cross General Hospital
2020
Red Cross Hospital
2020
Two forms of erythropoietin, EPO-bi and EPO-tetra, with different biological activities were isolated from the culture medium a recombinant Chinese hamster ovary cell line, B8-300, into which human erythropoietin gene had been introduced. EPO-bi, an unusual form, showed only one-seventh in vivo activity 3 times higher vitro previously described EPO (standard EPO). In contrast, EPO-tetra both comparable to those standard EPO. same amino acid composition immunoreactivity. However, structural...
Abstract Expression of sialyl Lewisa is known to be increased in cancers the digestive organs. The determinant serves as a ligand for E-selectin and mediates hematogenous metastasis cancers. In contrast, disialyl Lewisa, which has an extra sialic acid attached at C6-position penultimate GlcNAc expressed preferentially on nonmalignant colonic epithelial cells, its expression decreases significantly malignant transformation. Introduction gene α2→6 sialyl-transferase responsible synthesis colon...
Human erythropoietin contains three Asn-type and one mucin-type sugar chains. That the branching structure of outer portion chains is correlated to its biological activity in vivo has been reported recently (Takeuchi, M., Inoue, N., Strickland, T. W., Kubota, Wada, Shimizu, R., Hoshi, S., Kozutsumi, H., Takasaki, Kobata, A. (1989) Proc. Natl. Acad. Sci. U. S. 86, 7819-7822). In this study, effect trimming on vitro hormone was examined by using several glycosidases. produced recombinant...
A yeast mutant capable of producing Man<sub>5</sub>GlcNAc<sub>2</sub> human compatible sugar chains on glycoproteins was constructed. An expression vector for α-1,2-mannosidase with the "HDEL" endoplasmic reticulum retention/retrieval tag designed and expressed in <i>Saccharomyces cerevisiae</i>. <i>in vitro</i>α-1,2-mannosidase assay Western blot analysis showed that it successfully localized reticulum. triple lacking three glycosyltransferase activities then transformed an vector. The...
Stomatal development in plants is regulated by defensin-like secretory epidermal patterning factor (EPF) peptide hormones. Only one of these, stomagen, a positive regulator, whereas EPF1, EPF2, and possibly others are negative regulators. Here we explore the structure–function relationships EPFs, integrating NMR semi-in vitro stomagen experiments. We show that composed loop scaffold containing three disulphide bonds. A mutant EPF2 positively regulates stomatal density on Arabidopsis...
An efficient synthesis of the antiviral and antioxidative chromene (1) was achieved. A small amount 1 could be derived from plastoquinones 2 3, major constituents brown alga, Sargassum micracanthum. By following synthetic scheme involving its application, many kinds analogs can synthesized for evaluation their biological activity mechanistic study. The total 1, started geranyl acetate protected 2-bromo-6-methylhydroquinone, executed with Sharpless asymmetric dihydroxylation introduction...
Abstract STAT5 molecules are key components of the IL-2 signaling pathway, deficiency which often results in autoimmune pathology due to a reduced number CD4+CD25+ naturally occurring regulatory T (Treg) cells. One consequences IL-2-STAT5 axis is up-regulation FOXP3, master control gene for Treg However, roles other subsets have not yet been elucidated. We recently demonstrated that enhanced IL-10 production through activation. This occurred two types human cells: novel type umbilical cord...
ABSTRACT Background The development of novel therapies for sarcoma is urgently required because most sarcomas are refractory to adjuvant therapy and the treatment options limited. Although antitumor effects blue light (BL) have been reported several malignant tumors, its impact on various remains unknown. In this study, we examined BL kinds bone soft tissue sarcomas. Methods We used human osteosarcoma, undifferentiated pleomorphic sarcoma, liposarcoma, myxofibrosarcoma cell lines, as well a...
Abstract IL-18 is a multifunctional cytokine playing various regulatory roles in the immune system including induced production. As part of our ongoing studies on molecular mechanisms IL-18-induced IFN-γ production, we have examined transcriptional regulation gene by human myelomonocytic cell line, KG-1. On basis DNA/protein binding, determined an IL-18-inducible NF-κB binding site located at −786 to −776 region (designated KBBsite). Transient transfection promoter-reporter constructs...
The consensus primary amino acid sequence for mucin-type O-glycosylation sites has not been identified. To determine the shortest motif required high level O-glycosylation, we prepared more than 100 synthetic peptides and assayed in vitro O-GalNAc transfer to serine or threonine these using a bovine colostrum UDP-N-acetylgalactosamine:polypeptideN-acetylgalactosaminyl transferase (O-GalNAcT). We chose PDAASAAP from human erythropoietin (hEPO) further systematic substitutions because it...
Abstract A significant number of CD4 + CD8 T cells were detected in intestinal intraepithelial lymphocytes (IEL) various strains rats including Wistar, WKA, BN, LEW and F344. The site the population IEL increased with age all we examined. Most bearing expressed no CD5 antigen young rats, while some aged CD45RB − phenotype. In germ‐free Wistar age‐associated increase was not evident, indicating that stimulation by microflora important for expansion IEL. Aged athymic F344 nude contained...
MicroRNAs (miRNAs) play important roles in regulating post-transcriptional gene repression a variety of immunological processes. In particular, much attention has been focused on their regulatory T (Treg) cells which are crucial for maintaining peripheral tolerance and controlling cell responses. Recently, we established novel type human Treg line, termed HOZOT, multifunctional exhibiting CD4+CD8+ phenotype. this study, performed miRNA profiling to identify signature miRNAs therein...
Tryptanthrin, a bioactive ingredient of Polygonum tinctorium Lour., is member the Indigo plant family and has potent cytocidal effects on various human leukemia cells in vitro. At low concentrations, tryptanthrin enhanced expression cell differentiation (CD) markers monocytic (U-937) promyelocytic (HL-60) indicative to monocytes/macrophages. Furthermore, nitroblue tetrazolium (NBT) reductive alpha-naphthyl butyrate esterase (NBE) activities were markedly increased after treatment....
Recent studies have shown that O-mannosyl glycans are present in several mammalian glycoproteins. Although knowledge on the functional roles of these is accumulating, their biosynthetic pathways poorly understood. Here we report identification and initial characterization a novel enzyme capable forming GlcNAc beta 1-2Man linkage, namely UDP-N-acetylglucosamine: O-linked mannose beta-1,2-N-acetylglucosaminyltransferase microsome fraction newborn rat brains. The transfers to beta-linked...
We report the identification, molecular cloning, and characterization of an endo-beta-N-acetylglucosaminidase from nematode Caenorhabditis elegans. A search C. elegans genome database revealed existence a gene exhibiting 34% identity to Mucor hiemalis (a fungus) (Endo-M). Actually, extract contained activity. The putative cDNA for (Endo-CE) was amplified by polymerase chain reaction Uni-ZAP XR library, cloned, sequenced. recombinant Endo-CE expressed in Escherichia coli exhibited substrate...