A5062685178- Glycosylation and Glycoproteins Research
- Muscle Physiology and Disorders
- Carbohydrate Chemistry and Synthesis
- Ubiquitin and proteasome pathways
- Galectins and Cancer Biology
- Monoclonal and Polyclonal Antibodies Research
- Proteoglycans and glycosaminoglycans research
- Genetic Neurodegenerative Diseases
- Lysosomal Storage Disorders Research
- Alzheimer's disease research and treatments
- Parathyroid Disorders and Treatments
- Protease and Inhibitor Mechanisms
- Bone and Dental Protein Studies
- Photosynthetic Processes and Mechanisms
- Cardiomyopathy and Myosin Studies
- Enzyme Structure and Function
- Endoplasmic Reticulum Stress and Disease
- Adipose Tissue and Metabolism
- Cellular transport and secretion
- Pancreatic function and diabetes
- Lipid Membrane Structure and Behavior
- Mitochondrial Function and Pathology
- Diabetes and associated disorders
- Cancer, Hypoxia, and Metabolism
- Calpain Protease Function and Regulation
Tokyo Metropolitan Institute of Gerontology
2015-2025
Tokyo Metropolitan Geriatric Hospital
2013-2023
Nihon University
1992-2022
Asanogawa General Hospital
2016-2019
Kyoto University
1989-2016
Kanazawa University
2004-2013
Tottori University
2013
Kogakuin University
2009
RIKEN
2008
Tokyo Metropolitan University
2008
alpha-Dystroglycan is a heavily glycosylated protein, which localized on the Schwann cell membrane as well sarcolemma, and links transmembrane protein beta-dystroglycan to laminin in extracellular matrix. We have shown previously that sialidase treatment, but not N-glycanase of bovine peripheral nerve alpha-dystroglycan greatly reduces its binding activity laminin, suggesting sialic acid O-glycosidically-linked oligosaccharides may be essential for this binding. In report, we analyzed...
The demographic structure of a country changes dramatically with increasing trends toward general population aging and declining birth rates. In Japan, the percentage elderly (aged ≥65 years) reached 25% in 2013; it is expected to exceed 30% 2025 reach 39.9% 2060. national total has been decreasing steadily since its peak 2008, fall order 80 million Of population, those aged ≥75 years accounted for 12.3% as 2013, this 26.9% As changes, disease therefore medical care demand changes. To...
Saliva contains a large number of proteins that participate in the protection oral tissue. Exosomes are small vesicles (30—100 nm diameter) with an endosome-derived limiting membrane secreted by diverse range cell types. We have recently demonstrated exosomes present human whole saliva. In this study, we found saliva contained at least two types (exosome I and exosome II) different size protein composition. Proteomic analysis revealed both Alix, Tsg101 Hsp70, all exosomal markers,...
Glycosylation is an essential post-translational modification that underlies many biological processes and diseases. α-dystroglycan (α-DG) a receptor for matrix synaptic proteins causes muscular dystrophy lissencephaly upon its abnormal glycosylation (α-dystroglycanopathies). Here we identify the glycan unit ribitol 5-phosphate (Rbo5P), phosphoric ester of pentose alcohol, in α-DG. Rbo5P forms tandem repeat functions as scaffold formation ligand-binding moiety. We show enzyme activities...
Defects in O -mannosylation of α-dystroglycan are thought to cause certain types congenital muscular dystrophies with neuronal migration disorders. Among these dystrophies, Walker–Warburg syndrome is caused by mutations the gene encoding putative protein -mannosyltransferase 1 (POMT1), which homologous yeast -mannosyltransferases. However, there no evidence that POMT1 has enzymatic activity. In this study, we first developed a method detect activity mammalian cells. Then, using method,...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTDiversity of oligosaccharide structures linked to asparagines the scrapie prion proteinTamao Endo, Darlene Groth, Stanley B. Prusiner, and Akira KobataCite this: Biochemistry 1989, 28, 21, 8380–8388Publication Date (Print):October 17, 1989Publication History Published online1 May 2002Published inissue 17 October 1989https://pubs.acs.org/doi/10.1021/bi00447a017https://doi.org/10.1021/bi00447a017research-articleACS PublicationsRequest reuse...
Type 1 fimbriae with mannose-specific lectins are widely distributed among members of the family Enterobacteriaceae and confer ability to attach a range host cells, including colonic epithelial cells. The mucosal surfaces protected by secretory immunoglobulin A (IgA), which agglutinates microorganisms prevents their attachment This action has been attributed specificity antigen-combining site immunoglobulins for bacterial viral surface components. Here, we report novel mechanism...
We used a liposome lysis assay to measure antibodies against panel of glycolipids. Antibodies one or more compounds were detected in 34 46 patients with multiple sclerosis, 19 31 systemic lupus erythematosus (SLE), and the majority cranial trauma cerebrovascular accidents. ganglioside GM1 asialo found most commonly, they frequently present same sera. The specificity was tested four sera that contained both anti-GM1 cross-reacted GM1, but converse not true. Among whose glycolipids,...
Abstract The β‐site amyloid precursor protein cleaving enzyme‐1 ( BACE 1), an essential protease for the generation of amyloid‐β (Aβ) peptide, is a major drug target Alzheimer's disease AD ). However, there concern that inhibiting 1 could also affect several physiological functions. Here, we show modified with bisecting N‐acetylglucosamine (Glc NA c), sugar modification highly expressed in brain, and demonstrate patients have higher levels Glc c on 1. Analysis knockout mice lacking...
Background As Japan's population continues to age, it is estimated that the number of people aged ≥75 years will exceed 20 million by 2025. Furthermore, over past 10 years, we have not reduced difference between life expectancy and healthy expectancy. Therefore, extension development a society are most urgent issues. In terms medical care, changing times inevitably led changes in disease structures demands; therefore, delivery system has had be changed meet these demands. dementia rapidly...
ABSTRACT Sexual transmission of HIV-1 requires virus adsorption to a target cell, typically CD4 + T lymphocyte residing in the lamina propria, beneath epithelium. To escape mucosal clearance system and reach its cells, has evolved strategies circumvent deleterious host factors. Galectin-1, soluble lectin found underlayers epithelium, increases infectivity by accelerating binding susceptible cells. By comparison, galectin-3, family member expressed epithelial cells part system, does not...
Producing many mature oocytes is of great importance for assisted reproductive technologies. In mice, superovulation by consecutive injections equine chorionic gonadotropin (eCG) and human (hCG) has been the gold standard oocyte collection. However, yield this regimen can fluctuate according to stage estrous cycle, strain, age. Therefore, our objective was develop a high-yield protocol collect higher numbers from adult female mice different strains ages. First, we aimed synchronize cycle...
Significance Congenital muscular dystrophy (CMD) is caused by hypoglycosylation of α-dystroglycan (α-DG). In some CMD patients, mutations in the gene encoding protein O -linked mannose β1,2- N -acetylglucosaminyltransferase 1, POMGnT1 , are responsible for such hypoglycosylation. Many CMD-related genes thought to be involved glycosylation core M3, a specific -mannose–type structure α-DG. Although has long been known associated with CMD, its role M3 remains unclear. Our results reveal that...
We have now produced mouse-human chimeric IgG1 in wild-type Chinese hamster ovary (CHO) cell lines Pro-5 as well the glycosylation mutants Lec 2, 8, and 1. Analysis of attached carbohydrates shows those present on IgG1-Lec 1 were mannose terminated. Carbohydrate IgG1-Lec8 was uniformly biantennary terminating N-acetylglucosamine. The profiles 2 IgG1-Pro-5 heterogeneous. Only sialylated with sialic acid only a small percentage carbohydrate structures. When vivo fate antibodies labeled...
Cyclic electron transport around PSI through the NAD(P)H dehydrogenase complex (NDH) in tobacco leaf disks, measured as an increase dark level of Chl fluorescence after onset darkness, was inhibited by antimycin A, inhibitor ferredoxin quinone reductase (FQR), suggesting that A inhibits not only FQR-mediated cyclic flow but also NDH-dependent flow. This amytal, mitochondrial NDH and nigericin. The reduction plastoquinone detected when NADPH were added to suspension osmotically ruptured...