The Lewis blood group antigens of the human erythrocyte are acquired from plasma and not synthesized in situ . Although assumed previously to be glycoproteins, glycosphingolipids which taken up by membrane lipoproteins or aqueous dispersions.

In 2001, $17.8 billion was spent in the United States on dietary supplements, $4.2 of it for herbs and other botanical remedies.1 The popularity these products has increased over past decade, probably stimulated by sharp increases prices prescription drugs, restricted access to physicians imposed managed care, media reports adverse effects and, most important, enactment 1994 Dietary Supplement Health Education Act (DSHEA). By broadly defining botanicals as “dietary supplements,” DSHEA...

Noroviruses are a major cause of epidemic acute nonbacterial gastroenteritis worldwide. Here we report our discovery that recombinant Norwalk virus virus-like particles (rNV VLPs) agglutinate red blood cells (RBCs). Since histo-blood group antigens expressed on gut mucosa as well RBCs, used rNV VLP hemagglutination (HA) model system for studying NV attachment to in order help identify potential receptor(s). HA is dependent low temperature (4 degrees C) and acidic pH. Of the 13 species RBCs...

We used a liposome lysis assay to measure antibodies against panel of glycolipids. Antibodies one or more compounds were detected in 34 46 patients with multiple sclerosis, 19 31 systemic lupus erythematosus (SLE), and the majority cranial trauma cerebrovascular accidents. ganglioside GM1 asialo found most commonly, they frequently present same sera. The specificity was tested four sera that contained both anti-GM1 cross-reacted GM1, but converse not true. Among whose glycolipids,...

There is conflicting evidence on the efficacy of traditional Chinese acupuncture (TCA), and role placebo effects elicited by acupuncturists' behavior has not been elucidated. We conducted a 3-month randomized clinical trial in patients with knee osteoarthritis to compare TCA sham examine communication styles.Acupuncturists were trained interact 1 2 styles: high or neutral expectations. Patients 3 style groups, waiting list, high, neutral, nested within style, twice week over 6 weeks. Sham...

Journal Article Measurement of Serum Ferritin by Radioimmunoassay: Results in Normal Individuals and Patients With Breast Cancer Get access Donald M. Marcus, Marcus Search for other works this author on: Oxford Academic PubMed Google Scholar Nira Zinberg JNCI: the National Institute, Volume 55, Issue 4, October 1975, Pages 791–795, https://doi.org/10.1093/jnci/55.4.791 Published: 01 1975 history Received: May Accepted: 17 June

Abstract The object of this study is to prepare antisera purified glycosphingolipids for immunocytochemical studies. Rabbits were immunized with ganglioside GM1 complexed either methylated BSA or a glycoprotein isolated from human erythrocytes, and asialo the erythrocyte glycoprotein. IgG IgM antibody fractions separated by chromatography on Sephadex G-200 their properties determined complement fixation. antibodies highly specific exhibited little cross-reactivity other glycolipids, but...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTStructure of the human erythrocyte blood group P1 glycosphingolipidMasaharu Naiki, Jones Fong, Robert Ledeen, and Donald M. MarcusCite this: Biochemistry 1975, 14, 22, 4831–4837Publication Date (Print):November 4, 1975Publication History Published online1 May 2002Published inissue 4 November 1975https://pubs.acs.org/doi/10.1021/bi00693a009https://doi.org/10.1021/bi00693a009research-articleACS PublicationsRequest reuse permissionsArticle...

The Dietary Supplement Health and Education Act of 1994 (DSHEA), which arbitrarily classified herbals other medicinal products as dietary supplements, obscured fundamental differences between two classes products. Authentic supplements to the diet, such multivitamins or calcium, have nutritional value are safe. Herbals used worldwide medicines, they do not supplement may cause severe adverse events, should be regulated medicines. DSHEA also prevented Food Drug Administration (FDA) from...

Abstract This study investigates the influence of exogenous antigenic stimulation on serum immunoglobulin levels and circulating natural antibodies against carbohydrate antigens. Thus, BALB/c mice, raised in a germ‐free environment fed chemically defined, ultrafiltered diet (GF‐CD), were employed. These mice had normal IgM levels, but IgG IgA approximately 5% conventionally reared littermates. The concentrations all four isotypes equally low. variable part heavy chains naturally occurring...

Abstract Objective To identify components of the patient‐doctor relationship associated with trust in physicians. Methods We assessed 102 patients systemic lupus erythematosus (SLE) or rheumatoid arthritis (RA) seen at publicly funded hospitals Houston, Texas. Patients completed a self‐response survey examining patient perceptions medical encounter and their Evaluated physicians' behaviors included: informativeness, sensitivity to concerns, reassurance support, patient‐centeredness,...

Erythrocytes of the rare Pk phenotype lack blood group P antigen, and p erythrocytes both antigens. On basis immunological data we suggested previously that antigens are glycosphingolipids globoside trihexosyl ceramide, respectively, have now confirmed these designations by chemical analysis lacking The contain only traces a marked excess ceramide in comparison with normal erythrocytes. an lactosyl other complex glycolipids. Our analyses also revealed gangliosides approximate chromatographic...

The erythrocyte PK and P blood group antigens have been identified as ceramide trihexoside (CTH), Gal-(alpha, 1 leads to 4)Gal(beta, 4)Glc-Cer, globoside, GalN-Ac(beta, 3)Gal(alpha, respectively, the following structure has proposed for P1 antigen: Gal(alpha, 4)GlcNAc(beta, 3)Gal(beta, 4)Glc-Cer. Although determinants identical terminal disaccharides, CTH did not inhibit anti-P1. glycolipid hydatid cyst glycoprotein inhibited agglutination of P1K erythrocytes by anti-P1 unabsorbed anti-P1PPK...

Purified rabbit antibodies to neutral glycolipids were analyzed for their binding mouse embryonal carcinoma cells (ECC) and preimplantation embryos. Antibodies globotetraosylceramide first bind 2 4-cell embryos reach a peak of staining intensity with morulae, whereas anti-Forssman late morulae then, most intensely, early blastocysts. We compared the monoclonal antibody that show although they react similarly ECC, do not so morulae: The stained weakly unevenly, antiserum produced uniformly...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInteractions of saccharides with concanavalin A. Mechanism binding α- and β-methyl D-glucopyranoside to A as determined by carbon-13 nuclear magnetic resonanceC. F. Brewer, H. Sternlicht, D. M. Marcus, P. GrollmanCite this: Biochemistry 1973, 12, 22, 4448–4457Publication Date (Print):October 1, 1973Publication History Published online1 May 2002Published inissue 1 October...

A method was described for the partial purification of beta galactosidase and glucosaminidase from Clostridium tertium culture supernatants. Treatment erythrocytes with preparations containing both enzymes decreases their ability to react anti-I cold agglutinins, Type XIV antipneumococcal horse serum. Erythrocytes blood group A1 are altered more rapidly extensively than O cells. The enzymatic treatment stroma results in a decrease absorb agglutinins release galactose N-acetylglucosamine as...