A5061930144- Glycosylation and Glycoproteins Research
- Electron Spin Resonance Studies
- Effects of Radiation Exposure
- Galectins and Cancer Biology
- Carbohydrate Chemistry and Synthesis
- Free Radicals and Antioxidants
- Biochemical effects in animals
- Extracellular vesicles in disease
- Endoplasmic Reticulum Stress and Disease
- RNA regulation and disease
- Redox biology and oxidative stress
- Mitochondrial Function and Pathology
- Radiation Effects and Dosimetry
- Cancer, Hypoxia, and Metabolism
- Ubiquitin and proteasome pathways
- Radiation Therapy and Dosimetry
- Heat shock proteins research
- Electrolyte and hormonal disorders
- Lanthanide and Transition Metal Complexes
- Diabetes and associated disorders
- Autophagy in Disease and Therapy
- Palliative Care and End-of-Life Issues
- Sirtuins and Resveratrol in Medicine
- Cell death mechanisms and regulation
- Ultrasound and Hyperthermia Applications
Tokyo Metropolitan Institute of Gerontology
2016-2025
Proteome Sciences (United Kingdom)
2016
Hyogo University of Health Sciences
2015
University of Health Sciences
2015
Nagoya Institute of Technology
2012
Kirin (Japan)
2009
Shibaura Institute of Technology
2007
Shimadzu (Japan)
2007
Genomics (United Kingdom)
2007
Chiba Hospital
2005
Saliva contains a large number of proteins that participate in the protection oral tissue. Exosomes are small vesicles (30—100 nm diameter) with an endosome-derived limiting membrane secreted by diverse range cell types. We have recently demonstrated exosomes present human whole saliva. In this study, we found saliva contained at least two types (exosome I and exosome II) different size protein composition. Proteomic analysis revealed both Alix, Tsg101 Hsp70, all exosomal markers,...
Treatment with taxanes for castration-resistant prostate cancer often leads to the development of resistance. It has been recently demonstrated that exosomes present in body fluids contain proteins and RNAs cells from which they are derived could serve as a diagnostic marker various diseases. In study, we aimed identify contained be markers progression taxane-resistance cancer. Exosomes were isolated by differential centrifugation culture medium taxane-resistant human PC-3 (PC-3R) their...
Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) has been used for high-throughput glycan profiling analysis. In spite of the biological importance sialic acids on nonreducing ends glycans, it is still difficult to analyze glycans containing acid residues due their instability and presence linkage isomers. this Article, we describe a one-pot purification/derivatization method employing newly developed linkage-specific derivatization MS-based with differentiation...
Growth inhibitory factor (GIF), a brain-specific member of the metallothionein family (MT-III), has been characterized as substance for neurotrophic factors in Alzheimer's disease brains. However, function GIF, other than inhibition factors, remains unknown. We demonstrate here that exogenous GIF prevents neurite extension cortical neurons early period differentiation and death differentiated caused by high oxygen exposure. Down-regulation with antisense S-oligonucleotides promoted neuronal...
SIRT2 is potently and selectively inhibited by <italic>in situ</italic>-generated KPM-2 (<bold>36</bold>)-ADP ribose conjugate.
This study explored the effects of 5 taste solutions (citric acid, sucrose, sodium chloride, caffeine, and glutamate) versus water on power frequency content swallowing submental surface electromyography (sEMG). Healthy subjects were presented with ml each tastants water. Data collected in 3 trials by using sEMG, which was then subjected to spectral analysis. Sour salt increased spectrum-integrated values total components. The low-frequency (below 10 Hz) trial significantly increased,...
Abstract The DJ-1 gene, a causative gene for familial Parkinson’s disease (PD), has been reported to have various functions, including transcriptional regulation, antioxidant response, and chaperone protease functions; however, the molecular mechanism associated with pathogenesis of PD remains elusive. To further explore function in PD, we compared protein expression profiles brain tissues from wild-type DJ-1-deficient mice. Two-dimensional difference gel electrophoresis analysis subsequent...
Alterations of the structure and/or amount glycans present on proteins are associated with many diseases. We previously demonstrated that changes in N-glycans alter Aβ production. In study, we focused relationship between Alzheimer's disease (AD) and O-glycan, another type glycan. The UDP-N-acetylgalactosamine:polypeptide N-acetylgalactosaminyltransferase (GalNAc-T) family functions first step mucin-type O-glycan synthesis. Analysis expression GalNAc-Ts human brain using real-time PCR...
Neuromuscular junction (NMJ) denervation is an early event preceding motor neuron loss in amyotrophic lateral sclerosis (ALS). Progressive of the NMJ leads to irreversible muscle weakness and atrophy. Muscle-specific kinase (MuSK), locally expressed at postsynaptic membrane NMJ, activated by agrin released from nerve terminals essential for maintenance regeneration. Here, we found that progression prior onset SOD1-93A mouse model ALS correlated with increased serum MuSK immunoreactivity...
OBJECTIVES: Ethical issues have recently been raised regarding the appropriate care of patients in persistent vegetative state (PVS) Japan. The purpose our study is to attitudes and beliefs Japanese physicians who experience caring for PVS. DESIGN AND SETTING: A postal questionnaire was sent all 317 representative members Japan Society Apoplexy working at university hospitals or designated teaching by Ministry Health Welfare. asked subjects what they would recommend three hypothetical...
Life-sustaining treatment at the end of life gives rise to many ethical problems in Japan. Recent surveys Japanese physicians suggested that they tend treat terminally ill patients aggressively. We studied why were reluctant withhold or withdraw life-support from and what affected their decisions.A qualitative study design was employed, using a focus group interview with seven physicians, gain an in-depth understanding attitudes rationales Japan regarding medical care life.Analysis revealed...
The objective of the present study is to identify proteins that change in extent modification with O-linked N-acetylglucosamine (O-GlcNAcylation) kidney from diabetic model Goto-Kakizaki (GK) rats, and discuss relation between O-GlcNAcylation pathological condition diabetes. O-GlcNAcylated were identified by two-dimensional gel electrophoresis, immunoblotting peptide mass fingerprinting. level these was examined immunoprecipitation, situ Proximity Ligation Assay (PLA). changed significantly...
Soluble royal jelly protein is a candidate factor responsible for mammiferous cell proliferation. Major 1 (MRJP1), which consists of oligomeric and monomeric forms, an abundant proliferative in jelly. We previously reported that MRJP1 oligomer has biochemical heat resistance. Therefore, the present study, we investigated effects several treatments (56, 65 96°C) on activity oligomer. Heat resistance studies showed molecular forms were slightly maintained until 56℃, but converted to...
Neuronal aging and neurodegenerative diseases are accompanied by proteostasis collapse, while cellular factors that trigger it not identified. Impaired mitochondrial transport in the axon is another feature of diseases. Using Drosophila , we found genetic depletion axonal mitochondria causes dysregulation protein degradation. Axons with showed abnormal accumulation autophagic defects. Lowering neuronal ATP levels blocking glycolysis did reduce autophagy, suggesting defects associated...