A5035494664- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Carbohydrate Chemistry and Synthesis
- Cytomegalovirus and herpesvirus research
- Pharmacological Effects of Natural Compounds
- Immunotherapy and Immune Responses
- Hormonal Regulation and Hypertension
- Natural product bioactivities and synthesis
- Mycobacterium research and diagnosis
- Probiotics and Fermented Foods
- Microbial Natural Products and Biosynthesis
- Glycosylation and Glycoproteins Research
- Esophageal Cancer Research and Treatment
- Exercise and Physiological Responses
- Gut microbiota and health
- Eicosanoids and Hypertension Pharmacology
- Advanced Glycation End Products research
- CAR-T cell therapy research
- Fungal Biology and Applications
- Heat shock proteins research
- NF-κB Signaling Pathways
- Leprosy Research and Treatment
- Pneumocystis jirovecii pneumonia detection and treatment
- Electrolyte and hormonal disorders
Kitasato University
2006-2023
Kanazawa Hospital
1995-2022
Kyushu University
1990-2000
Osaka City University
1999-2000
Ontario Institute for Cancer Research
1996-1999
University of Toronto
1996-1999
Amgen (Canada)
1996-1999
Lunenfeld-Tanenbaum Research Institute
1997
Mount Sinai Hospital
1997
University of Würzburg
1997
Platelet/endothelial cell adhesion molecule-1 (PECAM-1; CD31), a member of the Ig superfamily, is expressed strongly at endothelial cell-cell junctions, on platelets, and most leukocytes. CD31 has been postulated to play role in vasculogenesis angiogenesis, implicated as key mediator transendothelial migration To further define physiologic CD31, we used targeted gene disruption embryonic stem cells generate CD31-deficient mice. mice (CD31KO) are viable born expected Mendelian frequency,...
The interleukin-2 receptor β chain (IL-2Rβ) is expressed on a variety of hematopoietic cell types, including natural killer (NK) cells and nonconventional T lymphocyte subsets such as intestinal intraepithelial lymphocytes (IEL). However, the importance IL-2Rβ-mediated signaling in growth development these has yet to be clearly established. We have investigated IEL NK mice deficient for IL-2Rβ describe here striking defects cells. IL-2Rβ−/− exhibited an abnormal population, characterized by...
Flavonoids have beneficial activities which modulate oxidative stress, allergy, tumor growth and viral infection, stimulate apoptosis of cells. In addition to these activities, dietary flavonoids are able regulate acute chronic inflammatory responses. Here we describe new aspects regulatory mechanisms by suppress production necrosis factor-alpha (TNF-alpha) macrophages, microglial cells mast stimulated with lipopolysaccharide (LPS) others via toll-like receptors (TLRs), TNF-alpha-mediated...
Euglena gracilis Z is a micro-algae that used as food or nutritional supplement. Paramylon, the carbohydrate storage substance of has β-1, 3-glucan structure. and paramylon are reported to affect immune system. In this study, we investigated protective effects against influenza virus infection in mice. were administered mice 2% dietary mixture ad libitum. At 2 weeks after initiation administration, infected intranasally with A/PR/8/34 (H1N1). Survival rate was monitored 10 days infection....
The dual specific kinase SAPK/ERK1 (SEK1; mitogen-activated protein 4/Jun NH2 terminal [ JNK] kinase) is a direct activator of stress-activated kinases ([SAPKs]/JNKs) in response to CD28 costimulation, CD40 signaling, or activation the germinal center kinase. Here we show that SEK1−/− recombination-activating gene (RAG)2−/− chimeric mice have partial block B cell maturation. However, peripheral cells displayed normal responses IL-4, IgM, and cross-linking. T showed decreased proliferation...
The leukocyte integrin LFA-1 plays an important role in trafficking and the immune response. Using LFA-1-deficient mice, we demonstrate that regulates of lymphocytes to peripheral lymph nodes, and, a lesser degree, mesenteric nodes acute inflammatory sites. LFA-1, either because its initial adhesion and/ or passage leukocytes across endothelial cells, vital T lymphocyte neutrophil transendothelial migration. Neutrophils activated from mice were unable cross cell monolayers response chemokine...
Abstract A significant number of CD4 + CD8 T cells were detected in intestinal intraepithelial lymphocytes (IEL) various strains rats including Wistar, WKA, BN, LEW and F344. The site the population IEL increased with age all we examined. Most bearing expressed no CD5 antigen young rats, while some aged CD45RB − phenotype. In germ‐free Wistar age‐associated increase was not evident, indicating that stimulation by microflora important for expansion IEL. Aged athymic F344 nude contained...
The immunopharmacological activities of chemically synthesized lipid A-subunit analogs, 4-O-phosphono-D-glucosamine derivatives carrying different N- and 3-O-linked acyl groups, were investigated. None the synthetic compounds tested exhibited any detectable pyrogenicity at a dose 10 micrograms/kg. Weaker lethal toxicity in galactosamine-sensitized mice was detected 1 microgram per mouse for all except GLA-58. Among (RS) stereoisomers 3-O-tetradecanoyl (C14) group with N-linked acyloxyacyl...
To determine the molecular mechanisms of T cell stimulation by staphylococcal enterotoxin A (SEA), we examined expression receptor (TcR) V beta on cells from four strains mice stimulated in vitro with SEA, using flow cytometric analysis for number bearing 3, 6, 8, 11 and RNA blotting amount transcripts 1, 5 12. The blasts 1 or 12 were increased proliferating response to SEA C57BL/6 mice. In AKR/J mice, which contain few 11- 12-bearing due a tolerance self-MHC class II IE-antigens, 3...
Cytomegalovirus (CMV) causes severe opportunistic infection in immunocompromised hosts. The importance of conventional alphabeta T cells protection against CMV has been well documented. However, the role second T-cell population (which express gammadelta receptor) is not known. In present study, we analysed function and protective a murine cytomegalovirus (MCMV) model. After intraperitoneal with MCMV, number increased liver peritoneal cavity from day 3, reached peak on 5. showed an activated...
Journal Article Failure of T cell receptor Vβ negative selection in murine intestinal intra-epithelial lymphocytes Get access Shinji Murosakai, Murosakai Search for other works by this author on: Oxford Academic PubMed Google Scholar Yasunobu Yoshikai, Yoshikai 1Laboratory Germfree Life, Research Institute Disease Mechanism and Control, Nagoya University School Medicine65 Tsurumai-Cho, Showa-ku, 466, Japan Correspondence to: Y. Atsunori Ishida, Ishida Takao Nakamura, Nakamura Goro Matsuzaki,...
T‐cell receptor (TCR) α β + CD4 − CD8 (double‐negative; DN) T cells appear in the peritoneal cavity at an early stage of intraperitoneal (i.p.) infection with intracellular pathogen Listeria monocytogenes . In present report, we analysed developmental pathway and functions TCR DN using L. system. The appeared after i.p. adult‐thymectomized lethally irradiated bone marrow chimeras p56 lck ‐deficient mice. results demonstrated that can develop extrathymically a ‐independent...
Abstract We previously reported that glycopeptidolipid (GPL) isolated from Mycobacterium avium serovar 4 inhibited phagosome‐lysosome (P‐L) fusion when macrophages phagocytosed heat‐killed Staphylococcus aureus (SA). In the present study we analyzed underlying inhibitory mechanism of GPL coated on SA. Elimination oligosaccharide abrogated its activity. did not inhibit P‐L opsonized SA via complement receptors. The activity was competitively reduced by presence α‐methyl‐ D ‐mannoside and...
Biological activities of lipopolysaccharides (LPS) extracted from Bordetella pertussis, B. parapertussis and bronchiseptica were compared with those Escherichia coli LPS. The LPS preparations pertussis showed biological comparable to E. in terms lethal toxicity galactosamine-sensitized mice, pyrogenicity rabbits, mitogenicity C3H/He spleen cell cultures, macrophage activation, induction tumour necrosis factor. All the parapertussis, except mitogenicity, lower than gave greatest mitogenic...
Quercetin (QUER) and luteolin (LUTE) are dietary flavonoids capable of regulating the production cytokines, such as tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6). However, their mechanisms action not fully understood. In lipopolysaccharide-triggered (LPS)-triggered signaling via Toll-like receptor 4 (TLR4), QUER LUTE suppresses only degradation inhibitor κB (IκB), with resultant activation nuclear factor-κB (NF-κB), but also phosphorylation p38 Akt in bone marrow-derived macrophages...
Euglena gracilis Z is a microorganism classified as microalga and used food or nutritional supplement. Paramylon, the carbohydrate storage substance of E. Z, reported to affect immunological system. This study evaluated symptom-relieving effects paramylon in rheumatoid arthritis collagen-induced mouse model. The efficacy both substances was assessed based on clinical signs, well cytokine (interleukin [IL]-17, IL-6, interferon [IFN]-γ) levels lymphoid tissues. Additionally, knee joints were...
When hot-water extracts of mushroom Agaricus brazei MURRILL fruiting bodies (Agaricus extracts) were administered orally into Meth A-bearing mice, tumor growth was significantly inhibited compared with controls given water orally. Treatment for five successive days increased natural killer activity spleen cells in naïve BALB/c mice. In enhanced the induction antigen-specific cytotoxic T lymphocytes and interferon γ production, indicating that potentiate innate adaptive immunity.