A5080770847- Virus-based gene therapy research
- Polyomavirus and related diseases
- Asthma and respiratory diseases
- Immune Cell Function and Interaction
- Bacteriophages and microbial interactions
- Viral Infectious Diseases and Gene Expression in Insects
- Heat shock proteins research
- T-cell and B-cell Immunology
- Antifungal resistance and susceptibility
- RNA Interference and Gene Delivery
- Cytomegalovirus and herpesvirus research
- Monoclonal and Polyclonal Antibodies Research
- Immunotherapy and Immune Responses
- Plant Virus Research Studies
- Epigenetics and DNA Methylation
- Pediatric health and respiratory diseases
- Immune Response and Inflammation
- Pluripotent Stem Cells Research
- DNA Repair Mechanisms
- Microtubule and mitosis dynamics
- Molecular Biology Techniques and Applications
- Genomics, phytochemicals, and oxidative stress
- Mast cells and histamine
- CRISPR and Genetic Engineering
- Fungal Infections and Studies
Nihon University
2014-2024
Kurashiki Central Hospital
2023-2024
The University of Tokyo
1989-2024
Mie University
2024
Nihon Pharmaceutical University
2018-2023
Kyushu University
1991-2001
Tokai University
1994
Tottori University
1973-1978
Shimane Prefectural Institute of Public Health and Environmental Sciences
1974-1977
Salk Institute for Biological Studies
1972-1973
Abstract Cell lines were established from cultures derived Fischer rat embryos according to the transfer schedule described by Todaro and Green (1963) for mouse 3T3 cells where cell crowding serum exhaustion kept a minimum. growth rate did not decline greatly during course of successive 3‐day transfers. Like cells, possess very low saturation densities under standard culture conditions. A clonal line with relatively high plating efficiency was obtained one lines, 3Y1. In these cloned...
The transforming ability in 10% serum medium of the temperature-sensitive mutants simian virus 40 complementation group III (ts640 type mutants) was greatly reduced when infected rat 3Y1 cells were incubated at restrictive temperature degrees or first for 3 days and then shifted to permissive 33 degrees. Transformation did occur efficiently after incubation an initial 5 followed by a shift When growth properties transformed examined degrees, several aspects state rendered...
The profile of PC945, a novel triazole antifungal designed for administration via inhalation, was assessed in range vitro and vivo studies. PC945 characterized as potent, tightly binding inhibitor Aspergillus fumigatus sterol 14α-demethylase (CYP51A CYP51B) activity (50% inhibitory concentrations [IC50s], 0.23 μM 0.22 μM, respectively) with characteristic type II azole spectra. Against 96 clinically isolated A. strains, the MIC values ranged from 0.032 to >8 μg/ml, while those voriconazole...
Abstract Invasive pulmonary Aspergillosis is a leading cause of morbidity and mortality in immunosuppressed patients treatment outcomes using oral antifungal triazoles remain suboptimal. Here we show that combining topical PC945, novel inhaled triazole, with systemic known demonstrated synergistic effects against Aspergillus fumigatus (AF) an vitro human alveolus bilayer model the lungs neutropenic immunocompromised mice. Combination apical PC945 either basolateral posaconazole or...
The nucleotide sequence around the origin of replication in DNA mouse polyoma virus was determined by 32P labeling 3' terminus Hap II-5/Alu I-1 fragment, with use polymerase. result coincided our previous report on labeling, polynucleotide kinase, 5' II-5/Hha which corresponds to large part present I-1. A symmetrical (A+T)-rich region containing a five-A stretch (or five-T stretch) flanked two small regions 2-fold rotational axis symmetry. On comparison near that corresponding simian 40 DNA,...
Cytomegalovirus (CMV) causes severe opportunistic infection in immunocompromised hosts. The importance of conventional alphabeta T cells protection against CMV has been well documented. However, the role second T-cell population (which express gammadelta receptor) is not known. In present study, we analysed function and protective a murine cytomegalovirus (MCMV) model. After intraperitoneal with MCMV, number increased liver peritoneal cavity from day 3, reached peak on 5. showed an activated...
Abstract Rat 3Y1 cells arrested at early S by hydroxyurea traversed the remainder of and G2 completed mitosis after removal drug, irrespective absence serum from culture medium. When were deprived for a period between hydroxyurea, delayed entry into in presence serium second generation time length approximately equal to that deprivation. mitotic cells, which had been continously exposed next 24 hours then reexposed serum, more than (more deprivation). On other hand, already first less...
Background Anti-oxidant capacity is crucial defence against environmental or endogenous oxidative stress. Nuclear factor erythroid 2-related 2 (Nrf2) a redox-sensitive transcription that plays key defensive role and cytotoxic stress cellular senescence. However, Nrf2 signalling impaired in several aging-related diseases, such as chronic pulmonary obstructive disease (COPD), cancer, neurodegenerative diseases. Thus, novel therapeutics enhance are an attractive approach to treat these...
ABSTRACT The antifungal effects of the novel triazole PC1244, designed for topical or inhaled administration, against Aspergillus fumigatus were tested in a range vitro and vivo studies. PC1244 demonstrated potent activities clinical A. isolates ( n = 96) with MIC 0.016 to 0.25 μg/ml, whereas voriconazole was 0.5 μg/ml. strong tight-binding inhibitor recombinant CYP51A CYP51B (sterol 14α-demethylase) enzymes strongly inhibited ergosterol synthesis 50% inhibitory concentration 8 nM. effective...
The oral administration of a kampo herbal medicine, Hochu-ekki-to (TJ-41: Bu-Zhong-Yi-Qi-Tang) using water-supplying bottle resulted in slight but significant inhibition Meth A growth. TJ-41 with gastric gavage significantly enhanced the specific antitumor activity against at rechallenge on day 9. In tumor-neutralizing assay, tumor draining LN cells administered mice showed an A. cytolytic anti-Meth T lymphocyte was not detected spleen bearing and mice. natural killer (NK) naive could...
PC945 is a novel triazole optimized for lung delivery, and the objective of this study to determine effects intranasally dosed on Aspergillus fumigatus infection associated biomarkers in immunocompromised mice. PC945, posaconazole, or voriconazole was administered once daily days 0 3 (early intervention) 1 (late postinfection temporarily neutropenic A/J mice infected with A. fumigatus, bronchoalveolar lavage fluid (BALF) serum were collected day 3. The extended prophylaxis treatment (daily...
The effect of dietary conjugated linoleic acid (CLA) on hepatic lipid parameters in Sprague-Dawley rats was examined. When were fed a diet containing CLA at 0 (control), 1, or 2% the weight amount food given for 3 weeks, liver exhibited slight increase CLA-fed groups, although difference not significant. Lipid accumulation hepatocytes also demonstrated by electron microscopic observation. In addition, thiobarbituric reactive substances levels significantly higher 2 wt % group than other two...
Abstract It has been recognized that macrophages play an important role in controlling virus infection experimental animal models. To evaluate the of acute murine cytomegalovirus infection, spleen and liver were eliminated by intravenous injection liposomes containing a cytolytic agent, dichloromethylene diphosphonate. The depletion led to significant increase titer lungs both susceptible BALB/c resistant C57BL/6 mice during first three days after infection. In spleen, macrophage‐depleted...
At 4 wk after intraperitoneal inoculation of murine cytomegalovirus (MCMV) in adult BALB/c mice, MCMV remained detectable only the salivary glands. When T cells these mice were activated by a single injection anti-CD3 epsilon monoclonal antibody, died interstitial pneumonitis at 24-48 h injection, accompanied elevation serum levels TNF-alpha and IFN-gamma. However, undetectable lungs during period. Simultaneous cyclosporin A reduced such effects anti-CD3. In conclusion, although presence...
Cytomegalovirus (CMV) causes severe clinical manifestations in immunocompromised hosts; however, it remains unclear whether the virus itself is a cause of immunosuppression or involved as an opportunistic bystander pathogen. This study was performed to elucidate effect CMV infection on host's immune system. The double-positive thymocytes BALB/c mice inoculated with sublethal dose murine (MCMV) were extensively depleted by 10-micrograms amount anti-CD3 monoclonal antibody, while such unable...
ABSTRACT Expression of actin was examined in a cultured rat embryonic cell line 3Y1 and transformed lines that originated from 3Y1. An α-actin addition to cytoplasmic α- γ-actins detected by two-dimensional gel electrophoresis. This α-Actin hardly at all the transformants induced Rous sarcoma virus, v-H-ras oncogene or adenovirus type 12, while retained N-methyl-N′-nitro-N-nitrosoguanidine SV40, which are relatively low malignancy. Western Northern blot analyses established this smooth...