(–)-Epigallocatechin-3-gallate (EGCG), the principal polyphenol in green tea, has been shown to be a potent chemopreventive agent. Recently, 67-kDa laminin receptor (67LR) identified as cell surface for EGCG that mediates anticancer activity of EGCG. Indeed, expression 67LR confers responsiveness tumor cells; however, molecular basis <i>in vivo</i> is not entirely understood. Here we show (i) using direct genetic screen, eukaryotic translation elongation factor 1A (eEF1A) component...

Fucoidan is an active component of seaweed that has been shown to inhibit proliferation and induce apoptotic cell death in several tumor cells. However, the detailed mechanisms underlying this process have not yet elucidated. In present report, we investigated effect fucoidan on induction apoptosis human breast cancer MCF-7 Our data demonstrated reduced viable number cells a dose- time-dependent manner. contrast, did affect normal mammary epithelial Results from assay induced...

Abstract Epigallocatechin-3-gallate (EGCG), a major active polyphenol of green tea, has been shown to downregulate inflammatory responses in macrophages; however, the underlying mechanism not understood. Recently, we identified 67-kDa laminin receptor (67LR) as cell-surface EGCG that mediates anticancer action at physiologically relevant concentrations (0.1–1 μM). In this study, show molecular basis for downregulation TLR4 signal transduction by 1 μM macrophages. Anti-67LR Ab treatment or...

The 67-kDa laminin receptor (67LR) is a laminin-binding protein overexpressed in various types of cancer, including bile duct carcinoma, colorectal cervical and breast carcinoma. 67LR plays vital role growth metastasis tumor cells resistance to chemotherapy. Here, we show that functions as cancer-specific death receptor. In this cell pathway, cGMP initiated by activating the PKCδ/acid sphingomyelinase (PKCδ/ASM) pathway. Furthermore, upregulation was rate-determining process 67LR-dependent...

A sensitive and simultaneous analytical technique for visualizing multiple endogenous molecules is now strongly required in biological science. Here, we show the applicability of a matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) system getting chemically diverse metabolite profiles on single-mammalian cell. This ultrahighly MALDI-MS enabled spatially resolved detection broad range metabolites including nucleotides, cofactors, phosphorylated sugars, amino acids,...

MicroRNAs (miRNAs) are non-coding RNAs involved in various biological processes by regulating their target genes. Green tea polyphenol (-)-epigallocatechin-3-O-gallate (EGCG) inhibits melanoma tumor growth activating 67-kDa laminin receptor (67LR) signaling. To examine the effect of EGCG on miRNA expression cells, we performed microarray analysis. We showed that up-regulated miRNA-let-7b through 67LR cells. The EGCG-induced up-regulation let-7b led to down-regulation high mobility group A2...

In this study, we investigated the estrogenic activity of environmental estrogens by a competition binding assay using human recombinant receptor (hERβ) and proliferation MCF-7 cells sulforhodamine-B assay. assay, pharmaceuticals had stronger to hERβ than that some phytoestrogens (coumestrol, daidzein, genistein, luteolin, chrysin, flavone, naringenin) or industrial chemicals, but such as coumestrol strong 17α-ethynylestradiol (EE), tamoxifen (Tam), mestranol. diethylstilbestrol, EE, Tam,...

Abstract Tea contains a variety of bioactive compounds. In this study, we show that two O-methylated catechins, (-)-epigallocatechin-3-O-(3-O-methyl) gallate and (-)-epigallocatechin-3-O-(4-O-methyl) gallate, inhibit in vivo mast cell-dependent allergic reactions more potently than their nonmethylated form, (-)-epigallocatechin-3-O-gallate. Consistent with this, these catechins IgE/Ag-induced activation mouse cells: histamine release, leukotriene cytokine production secretion were all...

Benifuuki is a tea cultivar with an antiallergic effect stronger than that of Yabukita tea, the most popular green consumed in Japan. The effective compound (−)-epigallocatechin-3-O-(3-O-methyl)gallate (EGCG3′′Me), O-methylated derivative EGCG. This study examined antihypertensive effects EGCG3′′Me and tea. First, it was determined has significant inhibitory on activity angiotensin I-converting enzyme (ACE). Second, clinical trials showed suppressed high blood pressure to greater extent did...

Green tea has various health promotion effects. Although there are numerous cultivars, little is known about the differences in their nutraceutical properties. Metabolic profiling techniques can provide information on relationship between metabolome and factors such as phenotype or quality. Here, we performed metabolomic analyses to explore health-promoting attributes (bioactivity) of diverse Japanese green cultivars.We investigated ability leaf extracts from 43 cultivars inhibit...

EGCG [(-)-epigallocatechin-3-O-gallate], the major polyphenol of green tea, has cancer chemopreventive and chemotherapeutic activities. selectively inhibits cell growth induces apoptosis in cells without adversely affecting normal cells; however, underlying molecular mechanism vivo is unclear. In present study, we show that EGCG-induced apoptotic activity attributed to a lipid-raft clustering mediated through 67LR (67 kDa laminin receptor) significantly elevated MM (multiple myeloma)...

Background We previously identified the 67-kDa laminin receptor (67LR) as cell-surface conferring major green tea polyphenol (–)-epigallocatechin-3-O-gallate (EGCG) responsiveness to cancer cells. However, underlying mechanism for interaction between EGCG and 67LR remains unclear. In this study, we investigated possible role of EGCG-67LR responsible its bioactivities. Methodology/Principal Findings synthesized various peptides deduced from extracellular domain corresponding 102-295 region...

Tea catechins, such as (-)-epigallocatechin-3-O-gallate (EGCG), have been shown to effectively enhance immune activity and prevent cancer, although the underlying mechanism is unclear. Green tea catechins are instead converted catechin metabolites in intestine. Here, we show that these green CD4(+) T cell well natural killer (NK) activity. Our data suggest absence of a 4'-hydroxyl on this phenyl group (B ring) important for effect In particular, 5-(3',5'-dihydroxyphenyl)-γ-valerolactone...

The Ras/Raf/MEK/ERK pathway has been identified as a major, druggable regulator of melanoma. Mutational activation BRAF is the most prevalent genetic alteration in human melanoma, resulting constitutive melanoma hyperproliferation. A selective inhibitor showed remarkable clinical activity patients with mutated BRAF. Unfortunately, acquire resistance to inhibitor, highlighting urgent need for new treatment strategies. Green tea polyphenol (-)-epigallocatechin-3-O-gallate (EGCG) inhibits cell...

We previously found that the O-methylated derivative of (−)-epigallocatechin-3-O-gallate (EGCg), (−)-epigallocatechin-3-O-(3-O-methyl)-gallate (EGCG' '3Me), has potent antiallergic activity. The high-affinity IgE receptor, FcεRI, is at high levels on basophils and mast cells plays a key role in series acute chronic human allergic reactions. To understand mechanism action for EGCG' '3Me, effect '3Me cell surface expression FcεRI basophilic KU812 was examined. Flow cytometric analysis showed...

Royal jelly is known as a functional food containing many useful minerals. In this study, we found an anti-environmental estrogen activity of royal jelly. Bisphenol A (BPA) environmental that stimulates proliferation human breast cancer MCF-7 cells. inhibited the growth-promoting effect BPA on cells, even though it did not affect cells in absence BPA. addition, inhibiting was heat-stable.

In the present study, a high-throughput and nontargeted metabolomic technique using matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) was developed for rapid analysis of cellular metabolites. Either detection limit or linearity between concentrations several standards peak intensities examined, indicating lower than 10 fmol/well with high at low concentrations. To verify validity this method, metabolites from human acute lymphoblastic leukemia Jurkat cells were...