A5073599741- Tea Polyphenols and Effects
- Metabolomics and Mass Spectrometry Studies
- Microstructure and Mechanical Properties of Steels
- Phytochemicals and Antioxidant Activities
- Mass Spectrometry Techniques and Applications
- Mast cells and histamine
- Analytical Chemistry and Chromatography
- Helicobacter pylori-related gastroenterology studies
- Monoclonal and Polyclonal Antibodies Research
- Metal Alloys Wear and Properties
- Metallurgy and Material Forming
- Immune Cell Function and Interaction
- Tannin, Tannase and Anticancer Activities
- Gastrointestinal disorders and treatments
- Inflammatory mediators and NSAID effects
- High Temperature Alloys and Creep
- Gastroesophageal reflux and treatments
- Gastric Cancer Management and Outcomes
- Adipose Tissue and Metabolism
- Gastrointestinal Tumor Research and Treatment
- Cell Adhesion Molecules Research
- Immune Response and Inflammation
- Atherosclerosis and Cardiovascular Diseases
- Microstructure and mechanical properties
- Hydrogen embrittlement and corrosion behaviors in metals
Kyushu University
2015-2024
Okayama Central Hospital
2019
Fukuoka University
2015-2017
Sakakibara Hospital
2011-2016
Osaka Prefecture University
2014-2016
Kyushu University Hospital
2016
Okayama Prefecture
2014
Institute of Navigation
2010-2014
Kawasaki Medical School
2000-2013
Kawasaki Hospital
1986-2013
Abstract Epigallocatechin-3-gallate (EGCG), a major active polyphenol of green tea, has been shown to downregulate inflammatory responses in macrophages; however, the underlying mechanism not understood. Recently, we identified 67-kDa laminin receptor (67LR) as cell-surface EGCG that mediates anticancer action at physiologically relevant concentrations (0.1–1 μM). In this study, show molecular basis for downregulation TLR4 signal transduction by 1 μM macrophages. Anti-67LR Ab treatment or...
A sensitive and simultaneous analytical technique for visualizing multiple endogenous molecules is now strongly required in biological science. Here, we show the applicability of a matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) system getting chemically diverse metabolite profiles on single-mammalian cell. This ultrahighly MALDI-MS enabled spatially resolved detection broad range metabolites including nucleotides, cofactors, phosphorylated sugars, amino acids,...
BACKGROUND--The mechanism of ulceration in Crohn9s disease remains unknown. AIMS--To clarify the role follicle associated epithelium (FAE) colonic lymphoid nodules formation ulcers disease. METHODS--After identification and aphthoid lesions by magnifying colonoscopy, 76 biopsy specimens were obtained from 10 patients with three hyperplasia. This study correlated colonoscopic, electron microscopic, immunohistochemical findings specimens. RESULTS--In disease, scanning microscopy surrounded a...
Green tea has various health promotion effects. Although there are numerous cultivars, little is known about the differences in their nutraceutical properties. Metabolic profiling techniques can provide information on relationship between metabolome and factors such as phenotype or quality. Here, we performed metabolomic analyses to explore health-promoting attributes (bioactivity) of diverse Japanese green cultivars.We investigated ability leaf extracts from 43 cultivars inhibit...
Background We previously identified the 67-kDa laminin receptor (67LR) as cell-surface conferring major green tea polyphenol (–)-epigallocatechin-3-O-gallate (EGCG) responsiveness to cancer cells. However, underlying mechanism for interaction between EGCG and 67LR remains unclear. In this study, we investigated possible role of EGCG-67LR responsible its bioactivities. Methodology/Principal Findings synthesized various peptides deduced from extracellular domain corresponding 102-295 region...
Background The relationship between bile acid reflux into the stomach and risk of atrophic gastritis intestinal metaplasia is still not well understood. Towards obtaining a better understanding, concentrations acids were measured. Patients Methods This study was carried out with participation 14 facilities in J apan, 2283 samples collected. subjects equal to or higher than limit detection divided four groups size (group A : 0–25%, group B 26–50%, C 51–75%, D 76–100%). Thus, including control...
We previously found that the O-methylated derivative of (−)-epigallocatechin-3-O-gallate (EGCg), (−)-epigallocatechin-3-O-(3-O-methyl)-gallate (EGCG' '3Me), has potent antiallergic activity. The high-affinity IgE receptor, FcεRI, is at high levels on basophils and mast cells plays a key role in series acute chronic human allergic reactions. To understand mechanism action for EGCG' '3Me, effect '3Me cell surface expression FcεRI basophilic KU812 was examined. Flow cytometric analysis showed...
In the present study, a high-throughput and nontargeted metabolomic technique using matrix-assisted laser desorption ionization-mass spectrometry (MALDI-MS) was developed for rapid analysis of cellular metabolites. Either detection limit or linearity between concentrations several standards peak intensities examined, indicating lower than 10 fmol/well with high at low concentrations. To verify validity this method, metabolites from human acute lymphoblastic leukemia Jurkat cells were...
Spatiotemporal information about biomolecules is indispensable for precise pathological analysis, but it remains largely unclear. Here we show a novel analytical platform combing mass spectrometry imaging (MSI) with its complementary technique, liquid chromatography-mass (LC-MS), to elucidate more comprehensive metabolic behaviors, spatiotemporal information, in tissues. Analysis of rat transient middle cerebral artery occlusion (MCAO) brain tissue after ischemia-reperfusion was performed...
Understanding the spatial distribution of bioactive small molecules is indispensable for elucidating their biological or pharmaceutical roles. Mass spectrometry imaging (MSI) enables determination ionizable present in tissue sections whole-body single heterogeneous organ samples by direct ionization and detection. This emerging technique now widely used situ label-free molecular endogenous exogenous molecules. MSI allows simultaneous visualization many types including a parent molecule its...
Oxidative dysfunction in the metabolism has long been implicated diverse biological disorders. Although a substantial number of metabolic enzymes are targeted for inactivation by oxidative stress, identifying those targets remains difficult due to lack comprehensive observations acting through stress response. We herein developed metabolomics strategy using integrative liquid chromatography‐mass spectrometry ( LC ‐ MS ) and observing rapid metabolomic changes response hydrogen peroxide (H 2...
Information on spatiotemporal metabolic behavior is indispensable for a precise understanding of physiological changes and responses, including those ripening processes wounding stress, in fruit, but such information still limited. Here, we visualized the spatial distribution metabolites within tissue sections tomato (Solanum lycopersicum L.) fruit using matrix-assisted laser desorption/ionization-mass spectrometry imaging (MALDI-MSI) technique combined with matrix...
Abstract Green tea, a widely consumed beverage in Asia, contains green tea catechins effective against obesity, especially epigallocatechin-3- O -gallate (EGCG), but must be an impractically huge amount daily to elicit its biological effect. Meanwhile, citrus polyphenols have various physiological effects that could enhance EGCG functionality. Here we investigated the antiobesity effect of combination and α-glucosyl hesperidin, polyphenol, at doses not been previously reported exert by...
The major green tea catechin, (−)‐epigallocatechin‐3‐ O ‐gallate (EGCG), has a suppressive effect on the expression of high‐affinity IgE receptor FcϵRI, which is key molecule in IgE‐mediated allergic reactions. Here we show that EGCG binds to cell surface and highly associates with plasma membrane microdomains, lipid rafts, human basophilic KU812 cells. disruption these rafts caused reduction amount raft‐associated FcϵRI‐suppressive EGCG. We also found an ability inhibit phosphorylation...
Abstract IL-1β has been shown to play a pivotal role in the development of inflammatory disorders. We recently found that natural triterpene, ursolic acid (UA), enhanced MIF release from nonstimulated macrophages. In this study, we examined effects UA on production several cytokines resident murine peritoneal macrophages (pMφ). increased protein IL-1β, IL-6, and MIF, but not TNF-α, dose- time-dependent manners. This triterpene also strikingly induced activation p38 MAPK ERK1/2 together with...