A5087136130- Helicobacter pylori-related gastroenterology studies
- Cellular Mechanics and Interactions
- Gastrointestinal Tumor Research and Treatment
- Cellular transport and secretion
- Cell Adhesion Molecules Research
- Gastrointestinal disorders and treatments
- Wnt/β-catenin signaling in development and cancer
- Galectins and Cancer Biology
- Microtubule and mitosis dynamics
- Neuroendocrine Tumor Research Advances
- Organ and Tissue Transplantation Research
- Gastric Cancer Management and Outcomes
- Atmospheric chemistry and aerosols
- Lymphoma Diagnosis and Treatment
- Organ Transplantation Techniques and Outcomes
- Tumors and Oncological Cases
- Barrier Structure and Function Studies
- Eosinophilic Esophagitis
- Protein Kinase Regulation and GTPase Signaling
- Renal Transplantation Outcomes and Treatments
- Mycobacterium research and diagnosis
- Gastroesophageal reflux and treatments
- S100 Proteins and Annexins
- Hippo pathway signaling and YAP/TAZ
- Metastasis and carcinoma case studies
Kyushu University
2008-2024
Nagoya University
2006-2016
Shinshu University
1987-1998
Ikeda Municipal Hospital
1991
Abstract. In this study an integrated simulation of the global distribution and radiative forcing soil dust aerosols at Last Glacial Maximum (LGM) is performed with aerosol climate model, SPRINTARS. It compared another for present condition. The total emission flux LGM simulated to be about 2.4 times as large that in climate, deposition general agreement estimations from ice core marine sediment samplings though it appears underestimated over Antarctic. calculated direct forcings close zero...
The epithelial cell sheet functions as a barrier to prevent invasion of pathogens. It is necessary eliminate intercellular gaps not only at bicellular junctions, but also tricellular contacts, where three cells meet, maintain function. To that end, tight junctions between adjacent must associate closely possible, particularly contacts. Tricellulin an integral component (tTJs), the molecular mechanism its contribution function remains unclear. In this study, we revealed tricellulin...
Polarised cell migration is required for various behaviours and functions. Actin microtubules are coupled structurally distributed asymmetrically along the front-rear axis of migrating cells. CLIP-associating proteins (CLASPs) accumulate near ends at front cells to control microtubule dynamics cytoskeletal coupling. Regional inhibition GSK-3beta responsible this asymmetric distribution CLASPs. However, it not known how regulates activity CLASPs linkage between actin microtubules. Here we...
Cadherin trafficking controls tissue morphogenesis and cell polarity. The endocytic adaptor Numb participates in apicobasal polarity by acting on intercellular adhesions epithelial cells. However, it remains largely unknown how cadherin-based adhesion. Here, we found that directly interacted with p120 catenin (p120), which is known to interact E-cadherin prevent its internalization. accumulated at adhesion sites the apical membrane Depletion of impaired internalization, whereas depletion...
Background The relationship between bile acid reflux into the stomach and risk of atrophic gastritis intestinal metaplasia is still not well understood. Towards obtaining a better understanding, concentrations acids were measured. Patients Methods This study was carried out with participation 14 facilities in J apan, 2283 samples collected. subjects equal to or higher than limit detection divided four groups size (group A : 0–25%, group B 26–50%, C 51–75%, D 76–100%). Thus, including control...
Tight junctions (TJs) are cell-adhesion structures responsible for the epithelial barrier. We reported that accumulation of cholesterol at apical is required TJ formation [K. Shigetomi, Y. Ono, T. Inai, J. Ikenouchi, Cell Biol. 217 , 2373–2381 (2018)]. However, it unclear how accumulates and informs formation—and whether enrichment precedes or follows assembly claudins in first place. Here, we established an cell line (claudin-null cells) lacks TJs by knocking out claudins. Despite lack TJs,...
Migrating cells acquire front-rear polarity with a leading edge and trailing tail for directional movement. The Rac exchange factor Tiam1 participates in polarized cell migration the PAR complex of PAR3, PAR6, atypical protein kinase C. However, it remains largely unknown how is regulated contributes to establishment migrating cells. We show here that interacts directly talin, which binds activates integrins mediate their signaling. accumulated at adhesions manner dependent on talin complex....
Microtubules (MTs) play critical roles in various cellular events, including cell migration. End-binding proteins (EBs) accumulate at the ends of growing MTs and regulate MT end dynamics by recruiting other plus end–tracking (+TIPs). However, how EBs contribute to through +TIPs remains elusive. We focused on tau-tubulin kinase 2 (TTBK2) as an EB1/3-binding confirmed that TTBK2 acted a +TIP. identified MT-depolymerizing kinesin KIF2A novel substrate TTBK2. phosphorylated S135 intact cells...
Gastric biopsy materials of 4074 consecutive Japanese patients undergoing esophagogastroduodenoscopy were reviewed, along with those 15 Helicobacter heilmannii infection (11, chronic gastritis; four, mucosa‐associated lymphoid tissue (MALT) lymphoma). In four H. infection, the examined by transmission electronmicroscopy. Urea breath test (three patients) and antibody (five performed in infection. two MALT lymphoma, was eradicated. The prevalence 0.1% series. gastritis, gastric mucosa...
Adherens junctions (AJs) control epithelial cell behavior, such as collective movement and morphological changes, during development in disease. However, the molecular mechanism of AJ remodeling remains incompletely understood. Here, we report that conformational activation α-catenin is key event dynamic regulation remodeling. activates RhoA to increase actomyosin contractility at cell-cell junctions. This leads stabilization activated α-catenin, part through recruitment actin-binding...
Abstract The cytoplasm in mammalian cells is considered homogeneous. In this study, we report that the cytoplasmic fluidity regulated blebbing cells; of rapidly expanding membrane blebs more disordered than retracting blebs. increase bleb caused by a sharp rise calcium concentration. STIM-Orai1 pathway regulates rapid and restricted Conversely, activated ERM protein binds to Orai1 inhibit store-operated entry blebs, which results decreased calcium, reassembly actin cortex.
The organization of the Golgi apparatus is essential for cell polarization and its maintenance. polarity regulator PAR complex (PAR3, PAR6, aPKC) plays critical roles in several processes polarization. However, how participates regulating remains largely unknown. Here we demonstrate functional cross-talk with CLASP2, which a microtubule plus-end-tracking protein involved organizing ribbon. CLASP2 directly interacted PAR3 was phosphorylated by aPKC. In epithelial cells, knockdown either or...
Tiam1 is one of the most extensively analyzed activators small GTPase Rac. However, fundamental aspects its regulation are poorly understood. Here we demonstrate that functionally suppressed by internal interactions and PAR complex participates in full activation. The N-terminal region binds to protein-binding catalytic domains inhibit localization Atypical PKCs phosphorylate relieve intramolecular interactions, subsequent stabilization interaction with PAR3 allows it exert localized...
The microtubule (MT) cytoskeleton is essential for cellular morphogenesis, cell migration, and division. MT organization primarily mediated by a variety of MT-associated proteins. Among these proteins, plus-end-tracking proteins (+TIPs) are evolutionarily conserved factors that selectively accumulate at growing plus ends. Cytoplasmic linker protein (CLIP)-170 +TIP associates with diverse to determine the behavior ends their linkage intracellular structures, including mitotic chromosomes....
Constriction of the apical plasma membrane is a hallmark epithelial cells that underlies cell shape changes in tissue morphogenesis and maintenance integrity homeostasis. Contractile force exerted by cortical actomyosin network anchored to junctional complexes (AJC). In this study, we present evidence MAGI proteins, structural components AJC whose function remained unclear, regulate constriction through Par polarity proteins. We reveal MAGIs are required uniformly distribute Partitioning...
Using an array of histochemical and immunohistochemical techniques, we have characterized the various types mucins in normal, metaplastic neoplastic stomach, pancreas biliary ducts, normal esophagus, colon lung, tumors ovary. Gastric phenotypes were variously expressed cells these organs. Their expression was usually associated with organoid differentiation simulating gastric pyloric mucosa.In intramucosal carcinoma tissues, often proliferated forming structures resembling non-neoplastic...