The actin cytoskeleton usually lies beneath the plasma membrane. When membrane-associated is transiently disrupted or intracellular pressure increased, membrane detaches from cortex and protrudes. Such protruded regions are called blebs. However, molecular mechanisms underlying blebbing poorly understood. This study revealed that epidermal growth factor receptor kinase substrate 8 (Eps8) ezrin important regulators of rapid reassembly for initiation retraction Live-cell imaging local...

Adherens junctions (AJs) control epithelial cell behavior, such as collective movement and morphological changes, during development in disease. However, the molecular mechanism of AJ remodeling remains incompletely understood. Here, we report that conformational activation α-catenin is key event dynamic regulation remodeling. activates RhoA to increase actomyosin contractility at cell-cell junctions. This leads stabilization activated α-catenin, part through recruitment actin-binding...

A rapid, easy, and robust preparation method for mRNA/cDNA display using a newly designed puromycin-linker DNA is presented. The new linker structurally simple, easy to synthesize, cost-effective use in "in vitro peptide protein selection". An introduction of RNase T1 nuclease site the facilitates recovery displayed by an improvement efficiency ligating mRNAs efficient release from solid-phase (magnetic bead). For application demonstration, affinity selections were successfully performed....

When mechanical stresses, such as tensile, compressive, and frictional are applied to objects by various motions, they accumulated in materials. Conventional mechanoresponsive materials sensors detect one-time stress. However, the stresses not visualized or measured previous works. The present study demonstrated imaging sensing of only but also stresses. Polyurethane (PU) film was combined with 2D layered polydiacetylene (PDA), a stimuli-responsive color-changing polymer. PDA generally...

cDNA display is a powerful in vitro technology used to explore functional peptides and proteins from huge library by selection. In addition expediting the selection cycle using display, easy rapid analysis of selected candidate clones crucial for high-throughput screening proteins. this report, versatile puromycin-linker employing an ultrafast photo-cross-linker, 3-cyanovinylcarbazole nucleoside, introduced. Its utility both protein-protein interaction surface plasmon resonance (SPR) system...

BackgroundThe evaluation of neurological examination in clinical practice still remains qualitative or semi-quantitative, and the results often vary depending on an examiner's skill level are less objective. In this study, we developed a smartphone-based application to investigate quantifying examinations using hand-drawn spirals diagnose patients with tremor artificial intelligence (AI).MethodsThis study included 24 26 essential (ET) cerebellar disease (CD), respectively, 41 age-matched...

Double disulphide-bridged loops of peptide aptamer are indispensable for the amino group recognition.

We explore the advantage of Gaussian mixture model (GMM) for interpretation single particle diffraction patterns from X-ray free electron laser (XFEL) experiments. GMM approximates a biomolecular shape by superposition distributions. As Fourier transformation can be quickly performed, we efficiently simulate XFEL approximated structure models. report that resolution accurately reproduce is proportional to cubic root number Gaussians used in modeling. This behavior attributed correspondence...

The library size is critical for selection in evolutionary molecular engineering (directed evolution). Although cDNA display has become a promising vitro technology by overcoming the instability of mRNA display, it hindered low yields. In this study, we improved yield molecules carefully examining each step preparation process.We found that steric hindrance ribosomes binding to mRNA-protein fusion was interfering with biotin-streptavidin binding. Additionally, reducing buffer exchange...

Constrained peptides are an attractive class as affinity reagents or drug leads owing to their excellent binding properties. Many kinds of these peptides, such cyclic containing disulfide bridges, found in nature designed artificially by directed evolution. However, confirming the properties disulfide-rich can be generally difficult, because oxidative folding problems preparation steps. Therefore, a method for evaluating rapidly and easily is required. Here, we report easy rapid preparing...

Interleukin (IL)-27 is a multifunctional cytokine that belongs to the IL-6/IL-12 family and has potent antitumor activity through various mechanisms. Our novel findings indicate IL-27 directly acts on hematopoietic stem cells promotes their expansion differentiation into myeloid progenitors control infection eradicate tumors.

Layered organic polymers have intercalation capabilities and dynamic properties. In classical chemistry, the interlayer guests are intercalated in host layers via electrostatic interaction. The present work shows layered materials with host-guest interaction hydrogen bond. Polydiacetylene (PDA) exhibits color changes from blue to red application of external stimuli, such as thermal mechanical stresses. Here we report on a PDA containing 4,4'-bipyridyl space hydrogen-bonding guest. Whereas...

The transition metal-free O-arylation reactions of quinoxalin-2-ones with diaryliodonium salts have been achieved, and desired 2-aryloxyquinoxalines were readily obtained in moderate to high yields.This method proved be compatible a series as well set quinoxalin-2-ones.In terms pharmaceutical research process development, are highly important transformation, 1,2 their catalytic non-catalytic methods strenuously investigated. 3,4 Quinoxalin-2-ones tautomerizable amide moiety, O-arylated...

Invited for this month's cover is the group of Prof. Yuya Oaki, Keio University, Japan. The picture shows a layered polydiacetylene containing hydrogen-bonding 4,4'-bipyridyl guests formed through self-organization. In classical intercalation chemistry, interlayer are intercalated in host layers via rigid strong electrostatic interaction. weaker interaction hydrogen bond promotes dynamic motion directing thermoresponsive color changes wide temperature range. More information can be found...

cDNA display is a robust and versatile in vitro protein selection tool developed on the basis of mRNA display. In this tool, covalently linked with its coding via specially-designed puromycin-linker to improve stability molecule. As result stabilization, succeeded extending variety libraries (e.g., modified peptide library) target molecules cell surface molecules). Thus, technology will enable design functional proteins peptides which could not be evolved by previous tools.

To detect abnormal breathing in a patient, it is necessary to take an image of the patient's chest and abdomen analyze their movement. The ultimate goal this study develop system that determines triage level by measuring 3D deformation real time analyzing results. This time, considering there little texture information chest, we focused on Moire analysis effective for measurement such cases. In research, dynamic using sequences necessary. Therefore, sampling method, which often used recent...

Reaction of (2S,3R,5S,6S)-2-methoxy-3,5-dimethyl-tetrahydro-2H-pyran-6-carbaldehyde (α-8) with MeMgCl gave (6′S)-α-9 as a single isomer which was converted into (5S,6R)-epoxide (cis-12) by sequence reactions involving tosylation, ring-opening the reaction HS(CH2)3SH, and treatment NaOMe. When α-8 its β-anomer (β-8) respectively reacted MeTi(Oi-Pr)3 resulting alcohols were subjected to similar transformation above, (5S,6S)-epoxide (trans-12) obtained irrespective anomeric configuration. The...