A5011178432- Advanced Fluorescence Microscopy Techniques
- Advanced biosensing and bioanalysis techniques
- Allergic Rhinitis and Sensitization
- Epigenetics and DNA Methylation
- RNA and protein synthesis mechanisms
- Advanced Biosensing Techniques and Applications
- Indoor Air Quality and Microbial Exposure
- Glioma Diagnosis and Treatment
- Lichen and fungal ecology
- Monoclonal and Polyclonal Antibodies Research
- Quantum Dots Synthesis And Properties
- Photosynthetic Processes and Mechanisms
- Additive Manufacturing and 3D Printing Technologies
- Cancer-related molecular mechanisms research
- Cancer, Hypoxia, and Metabolism
- Marine Toxins and Detection Methods
- Gene expression and cancer classification
- Molecular Biology Techniques and Applications
- Hedgehog Signaling Pathway Studies
- Atmospheric chemistry and aerosols
- Click Chemistry and Applications
- Viral Infectious Diseases and Gene Expression in Insects
- Genomics and Chromatin Dynamics
- Synthetic Organic Chemistry Methods
- Pancreatic function and diabetes
Saitama University
2015-2025
Nagoya University
2012-2025
Japanese Red Cross Nagoya Daiichi Hospital
2022-2024
Nara National Research Institute for Cultural Properties
2022-2024
Keio University
2008-2024
University of Tsukuba
2016-2024
University of Glasgow
2023
Association of Research Libraries
2023
University of Hong Kong
2023
Florida Institute of Technology
2023
The first chordates appear in the fossil record at time of Cambrian explosion, nearly 550 million years ago. modern ascidian tadpole represents a plausible approximation to these ancestral chordates. To illuminate origins chordate and vertebrates, we generated draft protein-coding portion genome most studied ascidian, Ciona intestinalis . contains ∼16,000 genes, similar number other invertebrates, but only half that found vertebrates. Vertebrate gene families are typically simplified form ,...
Retrograde menstruation is a widely accepted cause of endometriosis. However, not all women who experience retrograde develop endometriosis, and the mechanisms underlying these observations are yet understood. Here, we demonstrated pathogenic role Fusobacterium in formation ovarian In cohort women, 64% patients with endometriosis but <10% controls were found to have infiltration endometrium. Immunohistochemical biochemical analyses revealed that activated transforming growth factor–β...
Intestinal glucose uptake is mainly performed by the sodium-dependent transporter, SGLT1. The transport activity of SGLT1 was markedly inhibited green tea polyphenols, this inhibitory being most pronounced in polyphenols having galloyl residues such as epicatechin gallate (ECg) and epigallocatechin (EGCg). Experiments using brush-border membrane vesicles obtained from rabbit small intestine demonstrated that ECg a competitive manner, although itself not transported via present results...
DNA is methylated at the dinucleotide CpG in genomes of a wide range plants and animals. Among animals, variable patterns genomic methylation have been described, ranging from undetectable levels (e.g., Caenorhabditis elegans ) to high global vertebrates. The most frequent pattern invertebrate however, mosaic methylation, comprising domains interspersed with unmethylated domains. To understand origin patterns, we examined distribution Ciona intestinalis genome. Bisulfite sequencing...
Fluorescent acceptors have been immobilized on nanoparticulate quantum dots (QDs), which serve in turn as their FRET donors. The broad excitation and narrow emission bands of QDs mark them having excellent potential donors for and, principle, differently colored could be excited simultaneously. present work describes the preparation operation FRET-based QD bioprobes individually able to detect actions protease, deoxyribonuclease, DNA polymerase, or changes pH. In addition, two such...
In this study, we report a green, one-step synthesis of fluorescent carbon quantum dots (PET-FCQDs) derived from polyethylene terephthalate (PET) waste using an environmentally friendly pyrolytic method. The PET-FCQDs were systematically characterized techniques such as UV-Vis spectroscopy, fluorescence ATR-FTIR, TGA, and microscope, confirming their nanoscale size (2–50 nm), rich functional groups thermal stability. Thermal stability dynamics evaluated by the Coats–Redfern method showed...
Intestinal glucose uptake is mainly performed by its specific transporters, such as SGLT 1, GLUT 2 and 5 expressed in the intestinal epithelial cells. By using human Caco-2 cells we observed that was markedly inhibited tea extracts. While several substances green seem to be involved this inhibition, catechins play major role epicatechin gallate (ECg) showed highest inhibitory activity. Since our did not express enough amount of most abundant transporter, effect ECg on 1 evaluated brush...
A set of 3423 expressed sequence tags derived from the Ciona intestinalis tailbud embryos was categorized into 1213 independent clusters. When compared with DNA Data Bank Japan database, 502 clusters them showed significant matches to reported proteins distinct function, whereas 184 lacked sufficient information be (including undefined function) and 527 had no similarities known proteins. Sequence similarity analyses in relation biosynthetic as well structure message population at this...
Abstract Chemoresistance is a major cause of high mortality and poor survival in patients with ovarian cancer (OVCA). Understanding the mechanisms chemoresistance urgently required to develop effective therapeutic approaches OVCA. Here, we show that expression long noncoding RNA, taurine upregulated gene 1 (TUG1), markedly samples from OVCA who developed resistance primary platinum‐based therapy. Depletion TUG1 increased sensitivity cisplatin cell lines, SKOV3 KURAMOCHI. Combination therapy...
To better understand the mechanism of plastid differentiation from chloroplast to chromoplast, we examined proteome and changes over four distinct developmental stages ‘Micro-Tom’ fruit. Additionally, discover more about relationship between fruit color differentiation, also analyzed compared results with those two other varieties, ‘Black’ ‘White Beauty’. We confirmed that proteins related photosynthesis remain through orange maturity stage ‘Micro-Tom’, learned thylakoids no longer exist at...
Random mutagenesis for the hyperthermophilic archaeon Thermococcus kodakarensis was established by insertion of an artificial transposon designed to allow easy identification transposon-inserted locus. The phenotypic screening applied isolation thermosensitive mutants T. kodakarensis, which resulted in 16 showing defective growth at supraoptimal temperature 93°C. high occurrence suggested that thermotolerance hyperthermophiles achieved a combination diverse gene functions. sites two-thirds...
Abstract Caspase‐3 is a well‐described protease with many roles that impact the fate of cell. During apoptosis, caspase‐3 acts as an executioner caspase important proteolytic functions lead to final stages programmed cell death. Owing this key role, exploited intracellularly target control apoptosis for therapeutic outcomes. Yet activation during challenged by other and (e.g., paracrine signaling). This brief report presents way track levels using flow cytometer measures excited state...
Background: Immune checkpoint inhibitors have marked antitumor effect. However, monotherapy benefits only a limited population of patients, and further improvement their effects is required. Here the therapeutic effect immune response during anti-PD-L1 plus cisplatin combination therapy were investigated in mouse model. Materials Methods: E.G7-OVA, expressing ovalbumin (OVA) gene as model tumor antigen, was subcutaneously inoculated into syngeneic mice treated with with/without cisplatin....
Abstract Overcoming drug resistance is one of the biggest challenges in cancer chemotherapy. In this study, we examine whether targeting long noncoding RNA taurine upregulated gene 1 (TUG1) could be an effective therapeutic approach to overcome pancreatic ductal adenocarcinoma (PDAC). TUG1 was expressed at significantly higher levels across 197 PDAC tissues compared with normal tissues. Overall survival patients who had undergone 5-FU–based chemotherapy shorter high group than low group....