A5103008274- Lymphoma Diagnosis and Treatment
- Multiple Myeloma Research and Treatments
- T-cell and Retrovirus Studies
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Immune Cell Function and Interaction
- CAR-T cell therapy research
- Molecular Sensors and Ion Detection
- Hepatitis C virus research
- Protein Degradation and Inhibitors
- HIV/AIDS drug development and treatment
- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- Ubiquitin and proteasome pathways
- Vector-Borne Animal Diseases
- Peptidase Inhibition and Analysis
- Analytical Chemistry and Sensors
- Palliative Care and End-of-Life Issues
- Glycosylation and Glycoproteins Research
- Gastric Cancer Management and Outcomes
- Head and Neck Cancer Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- Crystallization and Solubility Studies
- X-ray Diffraction in Crystallography
- Animal Disease Management and Epidemiology
Community Care
2009-2025
Nagoya City University Hospital
2014-2025
Nagoya City University
2016-2025
Saku Central Hospital
2009-2025
Yokohama Municipal Citizen's Hospital
2014-2024
Nagaoka University of Technology
2024
National Institute of Technology, Toyota College
2024
National Institute for Materials Science
2013-2023
Mizuho (Japan)
1995-2023
Kobe University
2014-2021
Abstract Hodgkin lymphoma (HL) is characterized by the presence of a small number tumor cells in rich background inflammatory cells, but contribution abundant nontumor to HL pathogenesis poorly understood. We showed that migratory CD4+ induced were hyporesponsive T-cell receptor stimulation and suppressed activation/proliferation effector T an autologous setting. further affected lymph nodes surrounded large lymphocytes expressing both CC chemokine 4 (CCR4) FOXP3. These findings indicate...
Intracellular signal transduction relies on spatial and temporal transmitter dynamics. To clarify the correlations of these molecules, multicolor-imaging has been widely used. However, in case applying multiple indicators a cell, spectral overlap prevents accurate quantitative analysis. Moreover, invasive (toxic) effect, localization, metabolism, as well photobleaching complicate situation. Here, we show that single-molecular multifluorescent probes can overcome problems. While intracellular...
Abstract Purpose: Adult T-cell leukemia/lymphoma (ATLL) has a very poor prognosis. We have developed the humanized defucosylated anti–CC chemokine receptor 4 (CCR4) monoclonal antibody KW-0761 as next generation immunotherapeutic agent. The first aim of present study was to evaluate whether antitumor activity would likely be sufficient for therapeutic clinical application against ATLL. second fully elucidate mechanism antibody-dependent cellular cytotoxicity (ADCC) mediated by this antibody....
Controversy exists as to the role of oxygen-derived free radicals in tissue injury and no-reflow phenomenon reperfusion after ischemia. In this study using an experimental rat model, left hepatic lobar ischemia followed by resulted increase serum glutamic pyruvic transaminase at 30 min with concomitant histological evidence hepatocellular necrosis 24 hr. vivo liver microcirculation, initial transient return blood flow, but stasis flow later developed sinusoids. Thus a microcirculation was...
Fluorescent acceptors have been immobilized on nanoparticulate quantum dots (QDs), which serve in turn as their FRET donors. The broad excitation and narrow emission bands of QDs mark them having excellent potential donors for and, principle, differently colored could be excited simultaneously. present work describes the preparation operation FRET-based QD bioprobes individually able to detect actions protease, deoxyribonuclease, DNA polymerase, or changes pH. In addition, two such...
The expression of the neural cell adhesion molecule (NCAM) (CD56, NKH-1) is a rare phenomenon in malignant lymphoma. Recently, several authors, including our group, described clinicopathologic, phenotypic, and genotypic features NCAM-positive tumors as unique subgroup within larger category hematolymphoid malignancies. Ten cases CD56+ angiocentric lymphoma occurring sites other than upper aerodigestive tract were studied for evaluating their characteristics. disease occurred six men four...
Abstract Deletions of the 13q14 chromosome region are associated with B-cell chronic lymphocytic leukemia (B-CLL) and several other types cancer, suggesting presence a tumor suppressor gene. In previous studies minimal deletion (MDR) was mapped to less than 300-kilobase (kb) interval bordered by markers 173a12-82 138G4/1.3R. For identification putative gene, entire MDR (approximately 347 kb) has been sequenced, transcribed regions have identified exon trapping, EST-based full-length...
The IRE1α-XBP1 pathway, a key component of the endoplasmic reticulum (ER) stress response, is considered to be critical regulator for survival multiple myeloma (MM) cells. Therefore, availability small-molecule inhibitors targeting this pathway would offer new chemotherapeutic strategy MM. Here, we screened ER stress-induced XBP1 activation, and identified toyocamycin from culture broth an Actinomycete strain. Toyocamycin was shown suppress thapsigargin-, tunicamycin- 2-deoxyglucose-induced...
Abstract We recently reported expression of the chemokine receptors CXC receptor 3 (CXCR3) and CC 4 (CCR4) in adult T-cell leukemia/lymphoma showed a preferential CCR4 its association with an unfavorable outcome. In present study, we extend our study to other subtypes T- NK-cell lymphoma, clarify whether characteristic pattern is obtained for each defined by WHO classification. CXCR3 were rarely expressed three well-defined subtypes, precursor T-lymphoblastic anaplastic lymphoma...
Adult T-cell leukemia/lymphoma (ATLL) is a peripheral neoplasm with dismal prognosis, and no optimal therapy has been developed. We tested the defucosylated chimeric anti-CC chemokine receptor 4 (CCR4) monoclonal antibody, KM2760, to develop novel immunotherapy for this refractory tumor. In presence of blood mononuclear cells (PBMCs) from healthy adult donors, KM2760 induced CCR4-specific antibody-dependent cellular cytotoxicity (ADCC) against CCR4-positive ATLL cell lines primary tumor...
The role of intracellular magnesium ions is high interest in the fields pharmacology and cellular biology. To accomplish dynamic three-dimensional imaging Mg2+, there a strong desire for development optimized Mg2+ fluorescent probes. In this paper we describe design, synthesis, application three novel probes KMG-101, -103, -104. compounds series feature charged beta-diketone as binding site specific fluorescein residue fluorophore that can be excited with an Ar+ laser such widely used...
There are no suitable small animal models to evaluate human antibody-dependent cellular cytotoxicity (ADCC) in vivo, due species incompatibilities. Thus, the first aim of this study was establish a tumor-bearing mouse model which immune cells can engraft and mediate ADCC, but where endogenous cannot ADCC. The second ADCC mediated these humanized mice by defucosylated anti-CC chemokine receptor 4 (CCR4) monoclonal antibody (mAb) we have developed is now phase I clinical trials. NOD/Shi-scid,...
Treatment with tenofovir is sometimes associated renal dysfunction. Limited information available on this side effect in patients small body weight, although the use of will spread rapidly Asia and Africa, where are likely to be smaller weight.In a single-center cohort, Japanese HIV infection who started tenofovir-containing antiretroviral therapy were retrospectively analyzed. The incidence tenofovir-associated dysfunction, defined as more than 25% decrement estimated glomerular filtration...
Little information is available on the incidence of renal stones with ritonavir-boosted atazanavir (ATV/r) use.In a single-center study, was compared between human immunodeficiency virus (HIV)-infected patients who commenced (ATV/r)-containing antiretroviral (ARV) therapy (the ATV/r group) and those were receiving other protease inhibitors PIs group). The effects estimated by univariate multivariate Cox proportional hazards regression models. Other possible risk factors evaluated analysis,...
Background. Tenofovir is a widely used antiretroviral drug although it can cause kidney tubular dysfunction (KTD). The aim of this study was to determine the association between polymorphisms in genes encoding transporters and KTD Japanese patients treated with tenofovir. Methods. tenofovir-induced 14 single nucleotide (SNPs) ABCC2, ABCC4, ABCC10, SCL22A6, ABCB1 investigated 190 patients. diagnosed by presence at least 3 abnormalities following parameters: fractional resorption phosphate,...
Abstract The nicotinamide adenine dinucleotide (NAD) derivatives NADH and NADPH are critical components of cellular energy metabolism operate as electron carriers. A novel fluorescent ubiquinone‐rhodol derivative (UQ‐Rh) was developed a probe for NAD(P)H. By using the artificial promoter [(η 5 ‐C Me )Ir(phen)(H 2 O)] 2+ , intracellular activation imaging NAD(P)H were successfully demonstrated. In contrast to bioorthogonal chemistry, this “bioparallel chemistry” approach involves interactions...