This paper presents an inkjet printing method for the fabrication of entire microfluidic multianalyte chemical sensing devices made from suitable quantitative analysis, requiring only a single apparatus. An device is used three-dimensional hydrophilic patterns (550-μm-wide flow channels) and areas (1.5 mm × 1.5 squares) on filter paper, by etching, thereby locally dissolving hydrophobic poly(styrene) layer obtained soaking in 1 wt % solution toluene. In second step, same to print "chemical...

Rapid, precise, and reproducible deposition of a broad variety functional materials, including analytical assay reagents biomolecules, has made inkjet printing an effective tool for the fabrication microanalytical devices. A ubiquitous office device as simple standard desktop printer with its multiple ink cartridges can be used this purpose. This Review discusses combination technology paper substrate microfluidic paper-based devices (μPADs), which have developed into fast-growing new field...

The newly synthesized Keio Fluors, which are based on boron-dipyrromethene (BDP), have excellent and useful optical properties: vivid colors sharp emission in the visible−near-infrared region (583−738 nm), high quantum yields (Φ: 0.56−0.98), extinction coefficients (185000−288000 M-1 cm-1), good photostabilities. These properties superior to many of existing fluorescent dyes such as rhodamines, cyanines, or other BDP-based dyes.

A new series of high-performance fluorophores named Keio Fluors (KFL), which are based on borondipyrromethene (BODIPY), reported. The KFL dyes cover a wide spectral range from the yellow (547 nm) to near-infrared (NIR, 738 region, and their emission wavelength could be easily subtly controlled simple molecular modifications only, without losing optical properties. This "tailor-made" synthetic strategy for tuning enabled creation fourteen with well-controlled colors (yellow, orange, red,...

A new nanoparticulate inorganic−organic hybrid-type positive contrast agent (CA), PGP/dextran-K01, was synthesized based on a GdPO4 inorganic core as relaxation-time-shortening moiety and dextran-coating, which generates monodispersibility in water, high effect by retaining large number of water molecules proximity the toxicity reduction in-vivo studies. This PGP/dextran-K01 nanoparticle has r1 r2 values significantly low r2/r1 value, 1.1, is unprecedented lowest value among existing CAs...

Intracellular signal transduction relies on spatial and temporal transmitter dynamics. To clarify the correlations of these molecules, multicolor-imaging has been widely used. However, in case applying multiple indicators a cell, spectral overlap prevents accurate quantitative analysis. Moreover, invasive (toxic) effect, localization, metabolism, as well photobleaching complicate situation. Here, we show that single-molecular multifluorescent probes can overcome problems. While intracellular...

This paper describes a new method for the fabrication of microfluidic paper-based analytical devices (μPADs) by inkjet printing alone. Microfluidic structures are patterned within less than 5 min on surface untreated filter hydrophobic UV curable acrylate composition made non-volatile and not readily flammable compounds. After ink penetration into curing 60 s, barriers formed. channels as narrow 272 ± 19 μm achieved. Printed patterns retain their aqueous liquid guiding functionality at least...

This work reports on fully integrated "sample-in-signal-out" microfluidic paper-based analytical devices (μPADs) relying bioluminescence resonance energy transfer (BRET) switches for analyte recognition and colorimetric signal generation. The use BRET-based antibody sensing proteins into vertically assembled layers of functionalized paper, their design enables sample volume-independent reagent-free operation, including on-device blood plasma separation. User operation is limited to the...

A fully inkjet-printed disposable and low cost paper-based device for potentiometric Na+- or K+-ion sensing has been developed. printed ionophore-based all-solid-state ion selective electrode on a graphene/poly(3,4-ethylenedioxythiophene) polystyrenesulfonate (G/PEDOT:PSS) nanocomposite solid contact all-solid state reference consisting of pseudosilver/silver chloride coated by lipophilic salt-incorporating poly(vinyl chloride) membrane overprinted with potassium have combined...

This paper describes a colorimetric sensor array for the discrimination of volatile amines. Analyte is achieved by combining two functional elements: (1) "chemical class-selective" single chromogenic sensing dye with selectivity amines in general, encapsulated into (2) polymer nanoparticles different polarities. The resulting has ability to distinguish one closely related amine from another, relying on polarity-based approach. In order achieve reproducible, cost efficient, and flexible...

"Distance-based" detection motifs on microfluidic paper-based analytical devices (μPADs) allow quantitative analysis without using signal readout instruments in a similar manner to classical analogue thermometers. To realize cost-effective and calibration-free distance-based assay of lactoferrin human tear fluid μPAD not relying antibodies or enzymes, we investigated the fluidic mobilities target protein Tb(3+) cations used as fluorescent reagent surface-modified cellulosic filter papers....

An inkjet-printed microfluidic paper-based analytical device (μPAD) for the detection of lactoferrin has been developed. The analyte concentration dependent fluorescence emission, caused by sensitization pre-deposited terbium (Tb3+) upon complexation with on paper device, is captured using a digital camera. dynamic response range (0.5–3 mg mL−1) and limit (0.30 μPAD are suitable analysis normal human tears eye disorders. Finally, concentrations in tear samples were analyzed μPADs assay...

Although the determination of calcium ions (Ca2+) is high importance to monitor water hardness, currently available devices for on-site analysis suffer from a lack user-friendliness and sensitivity. This work demonstrates fully inkjet-printed low-cost microfluidic paper-based analytical (μPADs) simple naked-eye colorimetric in drinking tap samples. The quantification Ca2+ relies on visual readout length colour-changed detection channel modified with ionophore-doped ion-selective optode...

Foodborne illnesses caused by the ingestion of contaminated foods or beverages are a serious concern due to millions reported cases per year. It is essential develop sensitive and rapid detection methods foodborne pathogens ensure food safety for producers consumers. Unfortunately, current techniques still suffer from time-consuming operations need highly skilled personnel. Here, we introduce dual colorimetric/electrochemical approach Salmonella enterica serovar typhimurium (S. typhimurium)...

The role of intracellular magnesium ions is high interest in the fields pharmacology and cellular biology. To accomplish dynamic three-dimensional imaging Mg2+, there a strong desire for development optimized Mg2+ fluorescent probes. In this paper we describe design, synthesis, application three novel probes KMG-101, -103, -104. compounds series feature charged beta-diketone as binding site specific fluorescein residue fluorophore that can be excited with an Ar+ laser such widely used...

The fluorescent acridinyl indicators 4-(9-acridinyl)-N-(5-hexenyl)-N-methylaniline (KD-F0011), 6-(9-acridinyl)-1,2,2,3-tetramethyl-2,3-dihydro- 1H-perimidine (KD-F0021), and 6-(9-acridinyl)-2-(3-butenyl)-1,2,3-trimethyl-2,3-dihydro-1H-perimidine (KD-F0022) were designed, synthesized, applied for highly sensitive optical determination of low-level water in organic solvents. All these dyes found useful as fluorescence the detection below 1% (v/v) different solvent media with a low limit 0.002%...

We report a novel near-infrared fluorescent calcium probe (KFCA), which has good optical properties such as intense NIR fluorescence emission (670 nm, QY: 0.24), excellent ON/OFF ratio (120-fold), and wavelength-compatibility with visible-light-emissive fluorophores (Fluo-4, DsRed2), is applicable for real-time dual-colour intracellular Ca2+ imaging.

This work demonstrates the fabrication of microfluidic paper-based analytical devices (µPADs) suitable for analysis sub-microliter sample volumes. The wax-printing approach widely used patterning paper substrates has been adapted to obtain high-resolution structures patterned in filter paper. achieved by replacing hot plate heating method conventionally melt printed wax features into simple lamination. technique, combination with consideration device geometry and influence cellulose fiber...

Although the magnesium ion (Mg(2+)) is one of most abundant divalent cations in cells and known to play critical roles many physiological processes, its mobilization underlying mechanisms are still unknown. Here, we describe a novel fluorescent Mg(2+) probe, "KMG-104-AsH", composed highly selective "KMG-104", fluorescence-recoverable "FlAsH", bound specifically tetracysteine peptide tag (TCtag), which can be genetically incorporated into any protein. This probe was developed for molecular...

This paper describes a paper-based analytical device allowing the direct semiquantitative interpretation of result chemical assay in form "text". The combined use classical colorimetric indicator system and an additional inert colorant enables versatile text-displaying detection mechanism on device. For proof-of-concept, urinary protein has been selected as model target. whole developed based printing techniques including wax printing, inkjet 3D printing. results user tests performed with...

This paper introduces the concept of "Drawing-PADs" (Drawing paper-based microfluidic analytical devices) allowing to intuitively evaluate urinary albumin (Alb) index, a clinically important parameter used for early detection renal deficiencies related diabetes, among others. To enable regular monitoring Alb simple examination method suitable self-diagnosis is highly desirable. The Drawing-PADs rely on simultaneous naked eye and creatinine (Cre) single device according distance-based PAD...