A5073896584- Barrier Structure and Function Studies
- Connexins and lens biology
- Caveolin-1 and cellular processes
- Wnt/β-catenin signaling in development and cancer
- S100 Proteins and Annexins
- Hippo pathway signaling and YAP/TAZ
- Cellular Mechanics and Interactions
- Nanopore and Nanochannel Transport Studies
- Sphingolipid Metabolism and Signaling
- Gut microbiota and health
- RNA regulation and disease
- RNA Research and Splicing
- Cell Adhesion Molecules Research
- Cancer-related molecular mechanisms research
- Extracellular vesicles in disease
- Lipid Membrane Structure and Behavior
- Cancer Cells and Metastasis
Kyushu University
2015-2024
The epithelial cell sheet functions as a barrier to prevent invasion of pathogens. It is necessary eliminate intercellular gaps not only at bicellular junctions, but also tricellular contacts, where three cells meet, maintain function. To that end, tight junctions between adjacent must associate closely possible, particularly contacts. Tricellulin an integral component (tTJs), the molecular mechanism its contribution function remains unclear. In this study, we revealed tricellulin...
Tight junctions (TJs) are essential cell adhesion structures that act as a barrier to separate the internal milieu from external environment in multicellular organisms. Although their major constituents have been identified, it is unknown how formation of TJs regulated. TJ depends on preceding adherens (AJs) epithelial cells; however, underlying mechanism remains be elucidated. In this study, loss AJs α-catenin-knockout (KO) EpH4 cells altered lipid composition plasma membrane (PM) and led...
Tight junctions (TJs) are cell-adhesion structures responsible for the epithelial barrier. We reported that accumulation of cholesterol at apical is required TJ formation [K. Shigetomi, Y. Ono, T. Inai, J. Ikenouchi, Cell Biol. 217 , 2373–2381 (2018)]. However, it unclear how accumulates and informs formation—and whether enrichment precedes or follows assembly claudins in first place. Here, we established an cell line (claudin-null cells) lacks TJs by knocking out claudins. Despite lack TJs,...
Epithelial junctions comprise two subdomains, the apical junctional complex (AJC) and adjacent lateral membrane contacts (LCs), that span majority of junction. The AJC is lined with circumferential actin cables, whereas LCs are associated less-organized filaments whose roles elusive. We found DAAM1, a formin family regulator, accumulated at LCs, its depletion caused dispersion these sites while hardly affecting cables. DAAM1 loss enhanced motility LC-forming membranes, leading to their...
Abstract Epithelial cells define the boundary between outside and inside of our body by constructing diffusion barrier. Tight junctions (TJs) epithelial function as barriers against invasion harmful microorganisms into human free water or ions from body. Therefore, formation TJs has to be strictly controlled in cells. However, molecular mechanisms governing this regulation are largely unknown. In study, we identified Ca 2+ /calmodulin-dependent protein kinase II (CaMKII) a regulator barrier...
Constriction of the apical plasma membrane is a hallmark epithelial cells that underlies cell shape changes in tissue morphogenesis and maintenance integrity homeostasis. Contractile force exerted by cortical actomyosin network anchored to junctional complexes (AJC). In this study, we present evidence MAGI proteins, structural components AJC whose function remained unclear, regulate constriction through Par polarity proteins. We reveal MAGIs are required uniformly distribute Partitioning...
Imaging the distribution of sphingomyelin (SM) in membranes is an important issue lipid-raft research. Recently we developed novel fluorescent SM analogs that exhibit partition and dynamic behaviors similar to native SM, succeeded visualizing lateral domain-segregation between SM-rich liquid-ordered (Lo) SM-poor liquid-disordered (Ld) domains. However, because contrast these two domains depends directly on their ratio for SMs, domain-separation becomes indeterminate when difference not great...
Abstract Lipids have the ability to self-organize but significance of this phenomenon in assembly membrane structures is unknown. We previously reported that cholesterol enrichment directs formation tight junctions (TJs), adhesion responsible for epithelial barrier (Shigetomi et al., 2018). However, it unclear how accumulates and informs TJ formation. Cholesterol typically vicinity apical cell Surprisingly, distribution unaltered an line lacks TJs, generated by suppressing expression...